Shape, membrane morphology, and morphodynamic response of metabolically active human mitochondria revealed by scanning ion conductance microscopy

• Eric Lieberwirth,
• Anja Schaeper,
• Regina Lange,
• Ingo Barke,
• Simone Baltrusch and
• Sylvia Speller

Beilstein J. Nanotechnol. 2025, 16, 951–967, doi:10.3762/bjnano.16.73

• in high density within the outer membrane, are observed. This absence can be attributed to membrane fluctuations in living and metabolically active systems, which exhibit amplitudes of several 10 nm. See Supporting Information File 1, Section S5, for more information. These fluctuations obscure the

• response to the ζ-potential. For more information regarding the connection of ζ-potential and membrane fluctuations, refer to Supporting Information File 1, Section S5. Insufficient immobilisation causes height intermittency The observed height intermittency effect originates from the combination of

• membrane fluctuations; S6: SEM measurements of the polystyrene microspheres; S7: Details of the imaging dishes and SICM setup; S8: SEM measurements and other details of the nanopipettes; S9: Further information about the hopping mode at our SICM setup. Supporting Information File 4: Additional experimental

The nanomorphology of cell surfaces of adhered osteoblasts

• Christian Voelkner,
• Mirco Wendt,
• Regina Lange,
• Max Ulbrich,
• Martina Gruening,
• Susanne Staehlke,
• Barbara Nebe,
• Ingo Barke and
• Sylvia Speller

Beilstein J. Nanotechnol. 2021, 12, 242–256, doi:10.3762/bjnano.12.20

• glass and on amine-covered surfaces. At the rim of lamellipodia, characteristic edge heights between 100 and 300 nm are observed. Power spectral densities of membrane fluctuations show frequency-dependent decay exponents with absolute values greater than 2 on living osteoblasts. We discuss the

• capability of apical membrane features and fluctuation dynamics in aiding the assessment of adhesion and migration properties on a single-cell basis. Keywords: cell adhesion; membrane fluctuations; osteoblast; plasma membrane nanomorphology; scanning ion conductance microscopy (SICM); Introduction

• apical plasma membrane surface. Several other morphological and dynamic parameters are evaluated, for example, cell edge heights, membrane surface roughness, and membrane fluctuations, and discussed with respect to cellular functions. Results and Discussion In Figure 1 we show a typical overview SICM