Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy

• Shanka Walia and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57

• stretching vibration of Si–O–Si. The FITC conjugation with hybrid NPs was studied by using fluorescence spectroscopy. Both FITC and FITC-γ-Fe2O3 NPs exhibited an emission peak at 516 nm. The biocompatibility of these hybrid NPs was observed by incubating rat mesenchymal stem cells (rMSCs) with these hybrid

• found that the NPs emitted green light at 510 nm. The magnetic studies suggested that the nanocomposites exhibited typical property of superparamagnetic iron oxide by shortening the relaxation time T2. The cell uptake experiment of these nanocomposites was performed with human mesenchymal stem cells

Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation

• Ivonne Brüstle,
• Thomas Simmet,
• Gerd Ulrich Nienhaus,
• Katharina Landfester and
• Volker Mailänder

Beilstein J. Nanotechnol. 2015, 6, 383–395, doi:10.3762/bjnano.6.38

• interaction in malignant cell lines. Here, we report on the influence of polymeric nanoparticles on human hematopoietic stem cells (hHSCs) and mesenchymal stem cells (hMSCs). In this study we systematically investigated the influence of polymeric nanoparticles on the cell functionality and differentiation

• ; mesenchymal stem cells; nanoparticles; Introduction Interaction of different stem cell types with nanomaterials has been of interest lately for several reasons. One of the reasons is that nanoparticles (materials with dimensions well below the micrometer range) have been proposed for labeling of primary

• ][8][9]. As these were not studied side-by-side, comparison of the results is not commensurable. Human mesenchymal stem cells (hMSCs) are a promising tool for cell-based therapeutic strategies because they can be isolated and expanded to high numbers in vitro, they possess the ability to self-renew

Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung slices

• Stephanie Hirn,
• Nadine Haberl,
• Kateryna Loza,
• Matthias Epple,
• Wolfgang G. Kreyling,
• Barbara Rothen-Rutishauser,
• Markus Rehberg and
• Fritz Krombach

Beilstein J. Nanotechnol. 2014, 5, 2440–2449, doi:10.3762/bjnano.5.253

• response in PCLS. However, these NPs induced cytotoxicity in human mesenchymal stem cells and peripheral blood mononuclear cells in vitro when incubated at a concentration of 25 µg/mL or higher [29][30]. Additionally, these NPs elicited a cytotoxic and inflammatory response in rat lungs 24 h after

Nanoparticle interactions with live cells: Quantitative fluorescence microscopy of nanoparticle size effects

• Li Shang,
• Karin Nienhaus,
• Xiue Jiang,
• Linxiao Yang,
• Katharina Landfester,
• Volker Mailänder,
• Thomas Simmet and
• G. Ulrich Nienhaus

Beilstein J. Nanotechnol. 2014, 5, 2388–2397, doi:10.3762/bjnano.5.248

• surface functionalizations and investigated their interactions with various human cell lines, in particular HeLa cells and mesenchymal stem cells (MSCs). Of note, these studies were carried out in phosphate buffered saline (PBS), pH 7.4, or serum-free DMEM, so that we could probe interactions between

• NPs by mesenchymal stem cells (MSCs) in PBS solution. Because the initial step of endocytosis, i.e., the encounter of the NP with the cell membrane, is expected to be sensitive to the NP surface functionalization, we have exposed MSCs to two types of well-defined, positively charged PS NPs with

Biopolymer colloids for controlling and templating inorganic synthesis

• Laura C. Preiss,
• Katharina Landfester and
• Rafael Muñoz-Espí

Beilstein J. Nanotechnol. 2014, 5, 2129–2138, doi:10.3762/bjnano.5.222

• phosphate/chitosan composite films were shown to influence the behavior of human mesenchymal stem cells. Lee et al. [79] studied the scaffold–cell interaction by changing the crystallinity and ratio of the calcium phosphate. Alginate is another of the gelling biopolymers used as a scaffold. An alginate

Effect of silver nanoparticles on human mesenchymal stem cell differentiation

• Christina Sengstock,
• Jörg Diendorf,
• Matthias Epple,
• Thomas A. Schildhauer and
• Manfred Köller

Beilstein J. Nanotechnol. 2014, 5, 2058–2069, doi:10.3762/bjnano.5.214

• both ionic and nanoparticulate silver on the differentiation of human mesenchymal stem cells (hMSCs) into adipogenic, osteogenic and chondrogenic lineages and on the secretion of the respective differentiation markers adiponectin, osteocalcin and aggrecan. Results: As shown through laser scanning

• -toxic concentrations. Therefore, more studies are needed to investigate the effects of silver species on cells at low concentrations during long-term treatment. Keywords: differentiation; differentiation marker; mesenchymal stem cells; nanoparticles; silver; Introduction Novel nanomaterials are being

• healing [8]. In our previous studies on the biological effects of Ag-NP (PVP-coated, 80 nm) on human mesenchymal stem cells (hMSCs), we have shown that cell activation could occur at elevated but non-toxic silver concentrations [9][10]. In addition, we have shown that hMSCs are able to ingest Ag-NP

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

• have investigated whether STXM can be applied to investigate the cellular uptake process of silver nanoparticles in human mesenchymal stem cells (hMSC). For this purpose, hMSC were grown on collagen-coated Si3N4-membranes and incubated for 24 h with O2-free aqueous dispersions of silver particles (c

Imaging the intracellular degradation of biodegradable polymer nanoparticles

• Anne-Kathrin Barthel,
• Martin Dass,
• Melanie Dröge,
• Jens-Michael Cramer,
• Daniela Baumann,
• Markus Urban,
• Katharina Landfester,
• Volker Mailänder and
• Ingo Lieberwirth

Beilstein J. Nanotechnol. 2014, 5, 1905–1917, doi:10.3762/bjnano.5.201

• biological environments, intracellular degradation processes have been examined only to a very limited extent. PLLA nanoparticles with an average diameter of approximately 120 nm were decorated with magnetite nanocrystals and introduced into mesenchymal stem cells (MSCs). The release of the magnetite

• ., number of detached magnetite crystals, and the number of nanoparticles in one endosome), we demonstrate the importance of TEM studies for such applications in addition to fluorescence studies (flow cytometry and confocal laser scanning microscopy). Keywords: biodegradation; mesenchymal stem cells; PLLA

• expected for the measured fluorescence intensity when compared to that measured. The majority of measured intensities lie below the expected values. Parallel to each cell experiment, the doubling time of our respective mesenchymal stem cells was determined to be 48 h in the presence of the respective PLLA

Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii

• Sören B. Gutekunst,
• Carsten Grabosch,
• Alexander Kovalev,
• Stanislav N. Gorb and
• Christine Selhuber-Unkel

Beilstein J. Nanotechnol. 2014, 5, 1393–1398, doi:10.3762/bjnano.5.152

• substrate. The increase of adhesion area with decreasing Young`s modulus is opposite to the behavior of human mesenchymal stem cells [33] but is in good agreement with the trend observed in studies on neural stem cell cultures [34]. This result is reasonable, as during the infection process, A. castellanii

Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches

• Fabian Herzog,
• Kateryna Loza,
• Sandor Balog,
• Martin J. D. Clift,
• Matthias Epple,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149

• proliferation and migration (chemotaxis) both decreased, and the release of cytokines was affected. Increased IL-8 and decreased IL-6 and vascular endothelial growth factor (VEGF) levels were detected at high Ag NP concentrations [65]. These studies however, were obtained with human mesenchymal stem cells

Nanodiamond-DGEA peptide conjugates for enhanced delivery of doxorubicin to prostate cancer

• Amanee D Salaam,
• Patrick Hwang,
• Roberus McIntosh,
• Hadiyah N Green,
• Ho-Wook Jun and
• Derrick Dean

Beilstein J. Nanotechnol. 2014, 5, 937–945, doi:10.3762/bjnano.5.107

• tissues, the ND-DGEA conjugates were designed to distinguish between cells that overexpress α2β1 integrin, bone metastatic prostate cancers cells (PC3), and cells that do not, human mesenchymal stem cells (hMSC). Utilizing the ND-DGEA+DOX system, the efficacy of 1 µg/mL and 2 µg/mL DOX doses increased

• ) was used to measure the zeta potential and hydrodynamic size of the ND before and after modification with DGEA and DOX; samples were prepared at a concentration of 200 µg/mL. Cell culture Human bone metastatic prostate cancer cells (PC3) and mesenchymal stem cells (hMSC) were acquired from American

Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats

• Nadine Haberl,
• Stephanie Hirn,
• Alexander Wenk,
• Jörg Diendorf,
• Matthias Epple,
• Blair D. Johnston,
• Fritz Krombach,
• Wolfgang G. Kreyling and
• Carsten Schleh

Beilstein J. Nanotechnol. 2013, 4, 933–940, doi:10.3762/bjnano.4.105

• were not assessed in these studies. Several in vitro studies dealt with the mechanism of cytokine induction after AgNP exposure. Incubation of human mesenchymal stem cells and of peripheral blood monocytes with the same PVP-AgNP that were used in the present study induced a concentration-dependent

• the rate of the dissolution of AgNP depends on the surface functionalization, concentration and temperature [37]. They found an increasing toxicity to human mesenchymal stem cells during the storage of AgNP solutions, explained by the increasing release of silver ions over time. The authors emphasized