Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy

• Gayathri Kandasamy,
• Elena N. Danilovtseva,
• Vadim V. Annenkov and
• Uma Maheswari Krishnan

Beilstein J. Nanotechnol. 2020, 11, 354–369, doi:10.3762/bjnano.11.26

• the polyplex was found to augment the cytotoxic effects of the chemotherapeutic agent 5-fluorouracil. Microarray analysis of the mRNA isolated from cells treated with free siRNA and the polyplex reveal that the VEGF silencing by the polyplex also altered the expression levels of several other genes

• that have been connected to the proliferation and invasion of lung cancer cells. These results indicate that the PVI complexes can be an effective agent to counter lung cancer. Keywords: anti-VEGF siRNA; gene silencing; lung cancer; microarray; poly(1-vinylimidazole); small interfering RNA (siRNA

• Signaling Technology, USA) were used in the study. Microarray consumables were purchased from Cell Signaling Technology, USA. RNAse-free water was used for preparation of buffers and all solutions. Methods Preparation of the PVI–siRNA polyplex A stock solution of PVI (100 mg/mL) was prepared using RNAse

Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP)

• Seyed Mohammad Mahdi Dadfar,
• Sylwia Sekula-Neuner,
• Vanessa Trouillet,
• Hui-Yu Liu,
• Ravi Kumar,
• Annie K. Powell and
• Michael Hirtz

Beilstein J. Nanotechnol. 2019, 10, 2505–2515, doi:10.3762/bjnano.10.241

• was spotted onto them via microchannel cantilever spotting (µCS). Based on the fluorescence measurements, the optimal microarray design was found and its sensitivity was determined. Keywords: alpha-fetoprotein (AFP); cancer biomarker; click chemistry; fluorescent immunosensor; hepatocellular

• optimal samples (route 5, 37 °C, 20 min) was measured. Here, the optimal microarray was incubated with different concentrations of the fluorescently labeled AFP (ranging from 12.5 to 800 µg/mL). The resulting fluorescence intensity curve and corresponding representative images are presented in Figure 5

• . Here, the fluorescence remaining after washing of the microarray is highly reduced (Figure S2b). Finally, it should be noted that unlabeled AFP can also be detected in this approach. For demonstration, unlabeled AFP was spotted by µCS and stained via the same type of biotinylated antibodies as

Experimental study of an evanescent-field biosensor based on 1D photonic bandgap structures

• Jad Sabek,
• Francisco Javier Díaz-Fernández,
• Luis Torrijos-Morán,
• Zeneida Díaz-Betancor,
• Ángel Maquieira,
• María-José Bañuls,
• Elena Pinilla-Cienfuegos and
• Jaime García-Rupérez

Beilstein J. Nanotechnol. 2019, 10, 967–974, doi:10.3762/bjnano.10.97

• the immobilization density by fluorescence microarray measurements, densities corresponding to a close-packed monolayer of the half-antibodies were obtained with standard deviation of 8% indicating a good reproducibility of the immobilization method [13]. Biosensing experiment Figure 7 shows the

Strategy to discover full-length amyloid-beta peptide ligands using high-efficiency microarray technology

• Clelia Galati,
• Natalia Spinella,
• Lucio Renna,
• Danilo Milardi,
• Francesco Attanasio,
• Michele Francesco Maria Sciacca and
• Corrado Bongiorno

Beilstein J. Nanotechnol. 2017, 8, 2446–2453, doi:10.3762/bjnano.8.243

• features of the used system [27]. An additional advantage is the ease of implementing on it consolidated coating methods for the hosting of biomolecules. In this work, for peptide immobilization in a microarray format, we propose one of the most common functionalization methods (an epoxysilane) although

• high vacuum in the TEM chamber dried them completely and all of the solute species precipitated on the grid carbon layer. After storage in the humidity chamber for one hour, the standard time used for the amyloid incubation in our microarray experiments, no fibrillar structures were observed, in

• standard microarray protocol (described in the Experimental section). After BSA blocking, the slides were scanned in order to check the fluorescence of the amyloid spots after the extensive washing involved in the blocking process. As shown in Figure 2A, bright amyloid spots are observed on the

Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

• Sylwia Sekula-Neuner,
• Monica de Freitas,
• Lea-Marie Tröster,
• Tobias Jochum,
• Pavel A. Levkin,
• Michael Hirtz and
• Harald Fuchs

Beilstein J. Nanotechnol. 2017, 8, 715–722, doi:10.3762/bjnano.8.75

• Rhodamine-PE (dwell times 3 to 0.1 s). The arrow indicates the intensity profile of dot features presented on the superimposed graph. Scale bar equals 50 µm. c) Relationship between spot radius and dwell time for the microarray shown in (b). a) Fluorescence image of the STV-FITC binding Biotin-PE containing

A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

• Maryam Daneshpour,
• Kobra Omidfar and
• Hossein Ghanbarian

Beilstein J. Nanotechnol. 2016, 7, 2023–2036, doi:10.3762/bjnano.7.193

• biomarkers for early cancer diagnosis and prognosis. So far, different strategies have been developed in this regard, such as quantitative reverse transcription PCR (qRT-PCR) [13], locked nucleic acid-based northern blot [14], microarray [15], or flowcytometry [16]. Although there have been advantages to

Unraveling the neurotoxicity of titanium dioxide nanoparticles: focusing on molecular mechanisms

• Bin Song,
• Yanli Zhang,
• Jia Liu,
• Xiaoli Feng,
• Ting Zhou and
• Longquan Shao

Beilstein J. Nanotechnol. 2016, 7, 645–654, doi:10.3762/bjnano.7.57

• . [49] found that intranasal instillation of TiO2 NPs can induce histopathological changes in the mouse brain, in which OS (MDA increases) and inflammatory responses (elevated expressions of TNF-α and IL-1β) were involved. Shimizu et al. [50] analyzed the brains of mouse offspring by cDNA microarray and

• systems, as measured by DNA microarray in neonatal mouse brain [60]. Mice performed poorly in the Y-maze test after a 60 d exposure to TiO2 NPs, and histopathological changes were observed in brain. Meanwhile, the intracellular content of trace elements (Ca, Mg, Na, K, Zn, and Fe) was disturbed. The

Mismatch detection in DNA monolayers by atomic force microscopy and electrochemical impedance spectroscopy

• Maryse D. Nkoua Ngavouka,
• Pietro Capaldo,
• Elena Ambrosetti,
• Giacinto Scoles,
• Loredana Casalis and
• Pietro Parisse

Beilstein J. Nanotechnol. 2016, 7, 220–227, doi:10.3762/bjnano.7.20

• properties of DNA base pairing. Although the understanding of the behaviour of nucleic acids on a solid surface has made huge progress from the seminal work of Southern [1] due to the rapid development of DNA microarray and DNA microarray-based techniques [2][3], there are still open questions and

Application of biclustering of gene expression data and gene set enrichment analysis methods to identify potentially disease causing nanomaterials

• Andrew Williams and
• Sabina Halappanavar

Beilstein J. Nanotechnol. 2015, 6, 2438–2448, doi:10.3762/bjnano.6.252

• enrichment analysis methods to derive essential features of altered lung transcriptome following exposure to NMs that are associated with lung-specific diseases. Several datasets from public microarray repositories describing pulmonary diseases in mouse models following exposure to a variety of substances

• models of lung diseases that were not included in the present study due to lack of publicly available data or failure to meet the criteria set by the present study (time points, mouse strain, microarray platforms used). The analysis is also limited to the gene symbols that were consistently investigated

• across the various microarray platforms from the different studies included in the analyses. Furthermore, the bicluster analysis is conditional to the two-fold change cut-off employed to create the binary matrix for the Bimax algorithm and the choice of the Bimax parameters. Modifying the fold cut-off to

Experiences in supporting the structured collection of cancer nanotechnology data using caNanoLab

• Stephanie A. Morris,
• Sharon Gaheen,
• Michal Lijowski,
• Mervi Heiskanen and
• Juli Klemm

Beilstein J. Nanotechnol. 2015, 6, 1580–1593, doi:10.3762/bjnano.6.161

• need for standards and databases to house the extensive amount of data generated by gene expression and sequencing experiments, yielding such efforts as the development of the minimum information about a microarray experiment (MIAME) [16]. As a result, the MIAME guideline, and others, have been adopted

Anticancer efficacy of a supramolecular complex of a 2-diethylaminoethyl–dextran–MMA graft copolymer and paclitaxel used as an artificial enzyme

• Yasuhiko Onishi,
• Yuki Eshita,
• Rui-Cheng Ji,
• Masayasu Onishi,
• Takashi Kobayashi,
• Masaaki Mizuno,
• Jun Yoshida and
• Naoji Kubota

Beilstein J. Nanotechnol. 2014, 5, 2293–2307, doi:10.3762/bjnano.5.238

• interacted with the TUBA4A gene encoded the tubulin α-4A chain. Two hours after administration, TXNIP became a hub gene, which was found to act as a key gene in the treatment of breast cancer that was not responsive to PTX. Four hours after administration, the mathematical model for the DNA microarray data

Porous polymer coatings as substrates for the formation of high-fidelity micropatterns by quill-like pens

• Michael Hirtz,
• Marcus Lyon,
• Wenqian Feng,
• Andrea E. Holmes,
• Harald Fuchs and
• Pavel A. Levkin

Beilstein J. Nanotechnol. 2013, 4, 377–384, doi:10.3762/bjnano.4.44

• 10.3762/bjnano.4.44 Abstract We explored the potentials of microarray printing using quill-like microcantilevers onto solid supports that are typically used in microspot printing, including paper, polymeric nitrocellulose and nylon membranes. We compared these membranes with a novel porous poly(2

• micrometres. The bromophenol blue arrays on HEMA support were used to detect the presence of bovine serum albumin (BSA). In the presence of BSA, the fluorescence spectrum observed from the bromophenol blue microarray exhibited a significant red shift of the maximum emission wavelength. Our results show that

• platforms for the microarray spotting using SPTs. A pattern of 10 × 10 spots with a 50 µm pitch and dwell time of 0.5 s was written on each of the substrates by using a 10 mM solution of phloxine B in isopropanol mixed with 30 vol % glycerol (87% in water) to prevent drying of the dye solution in the SPT