Beilstein J. Nanotechnol.2012,3, 464–474, doi:10.3762/bjnano.3.53
thermal evaporator (Kurt Lesker PVD 75), and subsequently cleaned by ozone plasma ashing (Emitech K-1050X).
Protein monolayer
Five tandem B-domains of staphylococcal protein A were expressed and purified from E. coli. The C-terminus of the terminal protein was modified with cysteine to enable protein
binding to the gold surface. Protein patterns were prepared by dry stamping of the tandem B-domains on to the gold substrate surface, by using a polyurethane (pUA) stamp (15 µm hexagon). The pUA stamp was UV cross-linked on a silicon master with hexagonal pattern features and, before each use, cleaned by
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Figure 1:
Amplitude ratio of the second to the first harmonic, plotted for different applied forces. The surf...
Beilstein J. Nanotechnol.2011,2, 374–383, doi:10.3762/bjnano.2.43
= 0.1 ms (for ease of comparison). Evidently, the curves shift toward longer times with increasing protein concentration, indicating that the effective size of the NPs grows due to protein adsorption. The effect is small, however, so precise data are needed for a quantitative analysis of proteinbinding
diameter of a single α-helix separated by 2 nm, we obtain an overall thickness of 5–6 nm for the protein corona, which closely matches the observed ΔRH (Figure 2c, Table 1). About 65 apoE4 molecules will attach to the NP upon complete formation of the protein corona (Table 1).
Proteinbinding affinity
The
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Figure 1:
Fluorescence intensity correlation curves of NPs dissolved in buffer solutions of (a, b) HSA, (c, d...