Beilstein J. Nanotechnol.2014,5, 1193–1202, doi:10.3762/bjnano.5.131
membrane by biotin-streptavidin linkages. This anchorage resulted in the formation of a cortex-like actin structure within the GUVs. However, the GUVs in this study were contaminated by agarose, which adhered to the lipid membrane. A very similar approach for growing GUVs with embedded biomolecules was
with an inert membrane (containing PEG-lipids) is homogeneous and displays a depletion zone underneath the membrane. (B) Liposomes, which contain biotinylated lipids, encapsulate networks of biotinylated actin filaments that are coupled to the membrane via biotin–streptavidin bonds. The scale bars
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Figure 1:
Schematic view of active integrin molecules linking the ECM to the actin cytoskeleton. The heads of...
Beilstein J. Nanotechnol.2012,3, 404–414, doi:10.3762/bjnano.3.47
streptavidin, a streptavidin horseradish peroxidase conjugate complex is bound via the linker molecule to the surface. In order to remove the remaining unbound peroxidase complex, the samples were washed six times in PBST buffer for 2 min each after 1 h incubation of the surface with the peroxidase complex
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Figure 1:
Schematic sketch displaying the growth mechanism of the silver EGNP: (a) the substrates are immobil...