Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

• Jennifer E. Francis,
• David Mason and
• Raphaël Lévy

Beilstein J. Nanotechnol. 2017, 8, 1238–1249, doi:10.3762/bjnano.8.125

• accessible. We therefore, looked to antigens present in more complex intracellular structures, including the focal adhesion protein talin and nuclear splicer marker SC35. Talin exists in a dynamic equilibrium with both a bound pool (forming focal adhesions) and a cytosolic pool. Using the same primary

• antibody, Alexa Fluor 488 labelled both the cytosolic pool of talin and the bound pool forming focal adhesions, whereas Qdot 625 appeared to only label the cytosolic regions (Figure 4). At the same time, a control sample was prepared, consisting of HeLa cells incubated simultaneously with an Alexa Fluor

• 488-Ab and Qdot 625-Ab, without prior addition of an anti-talin primary antibody (Figure S5 in Supporting Information File 1). Here, it appears that the low signal of Qdot 625, is an indication that that the Qdot 625-Ab actually binds non-specifically to cells. Unlike β-tubulin and talin, SC35 is not

Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii

• Sören B. Gutekunst,
• Carsten Grabosch,
• Alexander Kovalev,
• Stanislav N. Gorb and
• Christine Selhuber-Unkel

Beilstein J. Nanotechnol. 2014, 5, 1393–1398, doi:10.3762/bjnano.5.152

• several molecules, such as talin and vinculin as well as ion channels under discussion to serve as possible candidates involved in sensing the mechanical properties of the cellular microenvironment [14][15][16]. In contrast to mammalian cells, for eukaryotic protists, such as amoebae, there is still only

Model systems for studying cell adhesion and biomimetic actin networks

• Dorothea Brüggemann,
• Johannes P. Frohnmayer and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131

• actin networks and talin into model cells. Keywords: actin network; cell adhesion; giant unilamellar vesicle; integrin; lipid bilayer; synthetic cell; protein reconstitution; talin; Review Introduction Since Hooke first described a biological cell in 1665 tremendous progress has been made in

• mechanisms of integrin-mediated cell adhesion and the interaction of talin with lipid vesicles. 1. The role of integrins in cell adhesion Cellular adhesion is an important mechanism, which enables cells to bind to the extracellular matrix and to surrounding cells. This process is crucial in regulating cell

• environment and the adhesive state of the cell by transmitting chemical signals into the cytoplasm [10][11]. In addition to this outside-in signalling process integrins can undergo conformational changes, which are called inside-out activation. These changes are primarily induced by talin, a major actin