/ E-Alerts

Volatile emission and biosynthesis in endophytic fungi colonizing black poplar leaves

Christin Walther, Pamela Baumann, Katrin Luck, Beate Rothe, Peter H. W. Biedermann, Jonathan Gershenzon, Tobias G. Köllner and Sybille B. Unsicker

Beilstein J. Org. Chem. 2021, 17, 1698–1711. https://doi.org/10.3762/bjoc.17.118

Supporting Information

Supporting Information File 1: Sequences of isolated endophytic fungi and identification according to NCBI database, primer used in this study, representative total ion chromatograms of single endophytic volatile blend, mass spectra of unknown volatile organic compounds, and BUSCO analysis of Cladosporium sp. de novo assembly.
Format: PDF	Size: 947.6 KB	Download

Cite the Following Article

Volatile emission and biosynthesis in endophytic fungi colonizing black poplar leaves

Christin Walther, Pamela Baumann, Katrin Luck, Beate Rothe, Peter H. W. Biedermann, Jonathan Gershenzon, Tobias G. Köllner and Sybille B. Unsicker

Beilstein J. Org. Chem. 2021, 17, 1698–1711. https://doi.org/10.3762/bjoc.17.118

How to Cite

Walther, C.; Baumann, P.; Luck, K.; Rothe, B.; Biedermann, P. H. W.; Gershenzon, J.; Köllner, T. G.; Unsicker, S. B. Beilstein J. Org. Chem. 2021, 17, 1698–1711. doi:10.3762/bjoc.17.118

Download Citation

Citation data can be downloaded as file using the "Download" button or used for copy/paste from the text window below.
Citation data in RIS format can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Zotero.

File format:

RIS

BibTeX

Include:

Citation for the article above

Citation and complete reference list for the article above

Download

TY  - JOUR
A1  - Walther, Christin
A1  - Baumann, Pamela
A1  - Luck, Katrin
A1  - Rothe, Beate
A1  - Biedermann, Peter H. W.
A1  - Gershenzon, Jonathan
A1  - Köllner, Tobias G.
A1  - Unsicker, Sybille B.
T1  - Volatile emission and biosynthesis in endophytic fungi colonizing black poplar leaves
JF  - Beilstein Journal of Organic Chemistry
PY  - 2021///
VL  - 17
SP  - 1698
EP  - 1711
SN  - 1860-5397
DO  - 10.3762/bjoc.17.118
PB  - Beilstein-Institut
JA  - Beilstein J. Org. Chem.
UR  - https://doi.org/10.3762/bjoc.17.118
KW  - Ascomycota
KW  - Cladosporium
KW  - Salicaceae
KW  - terpene synthases
KW  - volatile organic compound (VOC)
N2  - Plant volatiles play a major role in plant–insect interactions as defense compounds or attractants for insect herbivores. Recent studies have shown that endophytic fungi are also able to produce volatiles and this raises the question of whether these fungal volatiles influence plant–insect interactions. Here, we qualitatively investigated the volatiles released from 13 endophytic fungal species isolated from leaves of mature black poplar (Populus nigra) trees. The volatile blends of these endophytes grown on agar medium consist of typical fungal compounds, including aliphatic alcohols, ketones and esters, the aromatic alcohol 2-phenylethanol and various sesquiterpenes. Some of the compounds were previously reported as constituents of the poplar volatile blend. For one endophyte, a species of Cladosporium, we isolated and characterized two sesquiterpene synthases that can produce a number of mono- and sesquiterpenes like (E)-β-ocimene and (E)-β-caryophyllene, compounds that are dominant components of the herbivore-induced volatile bouquet of black poplar trees. As several of the fungus-derived volatiles like 2-phenylethanol, 3-methyl-1-butanol and the sesquiterpene (E)-β-caryophyllene, are known to play a role in direct and indirect plant defense, the emission of volatiles from endophytic microbial species should be considered in future studies investigating tree-insect interactions.
ER  -

Presentation Graphic

Picture with graphical abstract, title and authors for social media postings and presentations.
Format: PNG	Size: 12.1 MB	Download

Citations to This Article

Up to 20 of the most recent references are displayed here.

Scholarly Works

Medina-van Berkum, P.; Albracht, C.; Bröcher, M.; Solbach, M. D.; Stein, G.; Bonkowski, M.; Buscot, F.; Heintz-Buschart, A.; Ebeling, A.; Eisenhauer, N.; El-Madany, T. S.; Huang, Y.; Kuebler, K.; Meyer, S. T.; Gershenzon, J.; Unsicker, S. B. Plant diversity shapes plant volatile emission differently at the species and community level. Cold Spring Harbor Laboratory 2025. doi:10.1101/2025.04.30.651392
Walther, C.; Vallet, M.; Reichelt, M.; Giri, P.; Rothe, B.; Negwer, E. J.; van Berkum, P. M.; Gershenzon, J.; Unsicker, S. B. A Fungal Endophyte Alters Poplar Leaf Chemistry, Deters Insect Feeding and Shapes Insect Community Assembly. Ecology letters 2025, 28, e70007. doi:10.1111/ele.70007
Naik, B. S.; Rekha., D.; Deshpande, S. R. Volatile Organic Compounds from Endophytic Fungi and Their Role in Plant Growth and Disease Management. Fungal Endophytes Volume I; Springer Nature Singapore, 2025; pp 241–259. doi:10.1007/978-981-97-7312-1_9
Laupheimer, S.; Ghirardo, A.; Kurzweil, L.; Weber, B.; Stark, T. D.; Dawid, C.; Schnitzler, J.-P.; Hückelhoven, R. Blumeria hordei affects volatile emission of susceptible and resistant barley plants and modifies the defense response of recipient plants. Physiologia plantarum 2024, 176, e14646. doi:10.1111/ppl.14646
Gupta, S.; Saxena, S. Endophytes: Saviour of apples from post-harvest fungal pathogens. Biological Control 2023, 182, 105234. doi:10.1016/j.biocontrol.2023.105234
Lu, S.; Deng, H.; Zhou, C.; Du, Z.; Guo, X.; Cheng, Y.; He, X. Enhancement of β-Caryophyllene Biosynthesis in Saccharomyces cerevisiae via Synergistic Evolution of β-Caryophyllene Synthase and Engineering the Chassis. ACS synthetic biology 2023, 12, 1696–1707. doi:10.1021/acssynbio.3c00024
El Jaddaoui, I.; Rangel, D. E. N.; Bennett, J. W. Fungal volatiles have physiological properties. Fungal biology 2023, 127, 1231–1240. doi:10.1016/j.funbio.2023.03.005

Explore citations to this article at

Back To Article

Other Beilstein-Institut Open Science Activities

aromatic	the word “aromatic”
aromatic aldehyde	the word “aromatic” OR “aldehyde”
+aromatic +aldehyde	both words “aromatic” AND “aldehyde”
+aromatic -aldehyde	the word “aromatic” but NOT “aldehyde”
“aromatic aldehyde”	the exact phrase “aromatic aldehyde”
benz*	words which begin with “benz”, such as “benzene” or “benzyl”
benz*yl	words that begin with “benz” and end with “yl”, such as “benzyl” or “benzoyl”
benzyl~	words that are close to the word “benzyl”, such as “benzoyl” (i.e., fuzzy search)