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3 article(s) from Müller, Jens

Incorporation of a metal-mediated base pair into an ATP aptamer – using silver(I) ions to modulate aptamer function

Marius H. Heddinga and
Jens Müller

Beilstein J. Org. Chem. 2020, 16, 2870–2879, doi:10.3762/bjoc.16.236

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Graphical Abstract

Figure 1: Chemical structure of the Im–Ag(I)–Im base pair [30].

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Figure 2: The aptamer derivatives used in this study. The imidazole deoxyribonucleotide is marked in green as...

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Figure 3: Temperature-dependent normalized UV absorbance at 260 nm for oligonucleotides a) 1af, b) 1bf, c) 1cf...

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Figure 4: The two systems devised to investigate the envisaged functionality of the modified structure-switch...

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Figure 5: System A: the aptamers 1af, 1bf, 1cf, and 1df with the complementary sequence 1q (X = imidazole, f ...

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Figure 6: Normalized fluorescence intensity at 526 nm (λ_exc = 490 nm) of a) 1af, b) 1bf, c) 1cf, and d) 1df p...

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Figure 7: System B: aptamers 1a, 1b, 1c, and 1d with the complementary oligonucleotides 2f and 2q (X = imidaz...

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Figure 8: Normalized fluorescence intensity at 526 nm (λ_exc = 490 nm) of the oligonucleotide 2f before and af...

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Figure 9: Elution of the aptamer derivatives from the ATP-agarose gel in the absence of Ag(I): 1af (red), 1bf...

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Figure 10: Elution of the aptamers from the ATP-agarose gel in the presence of 1 equiv of Ag(I): 1af (red), 1bf...

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Full Research Paper

Published 25 Nov 2020

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Metal-mediated base pairs in parallel-stranded DNA

Jens Müller

Beilstein J. Org. Chem. 2017, 13, 2671–2681, doi:10.3762/bjoc.13.265

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Figure 1: Structure of a B-DNA duplex comprising a central C–Ag(I)–C base pair. This figure was created based...

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Scheme 1: Hydrogen-bonding patterns of various reversed Watson–Crick base pairs. a) A:T; b) G:C (steric clash...

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Scheme 2: Various hydrogen-bonding patterns. a) T:A:T and CH⁺:G:C base triples indicating how T:A and CH⁺:G H...

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Scheme 3: Representation of a β-dA:α-dT base pair.

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Scheme 4: Representation of mononuclear metal-mediated base pairs involving canonical pyrimidine nucleobases....

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Scheme 5: Proposed base pairing patterns of FP–Ag(I)–FP, involving a) the Watson–Crick edge (antiparallel-str...

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Scheme 6: Reaction pathway towards a parallel-stranded DNA duplex bearing a G–Pt(II)–G base pair. Adapted fro...

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Scheme 7: Possible base pairing patterns of dinuclear Ag(I)-mediated base pairs. Base pairs within an antipar...

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Scheme 8: Base pairing patterns of the dinuclear Ag(I)-mediated homo base pair of 1,N⁶-ethenoadenine (εA) wit...

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Scheme 9: Additional metal-mediated base pairs involving 1,N⁶-ethenoadenine (εA). Base pairs within an antipa...

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Review

Published 13 Dec 2017

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Second generation silver(I)-mediated imidazole base pairs

Susanne Hensel,
Nicole Megger,
Kristina Schweizer and
Jens Müller

Beilstein J. Org. Chem. 2014, 10, 2139–2144, doi:10.3762/bjoc.10.221

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Scheme 1: Schematic representation of an imidazole–Ag(I)–imidazole base pair (top) and a thyminate–Hg(II)–thy...

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Figure 1: Different views of a DNA duplex comprising three neighbouring imidazole–Ag(I)–imidazole base pairs....

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Scheme 2: Schematic representation of silver(I)-mediated imidazole homo base pairs involving 2-methylimidazol...

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Scheme 3: Synthesis of methylimidazole-based nucleosides and their corresponding phosphoramidites required fo...

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Scheme 4: Oligonucleotide sequences under investigation (X = 2-methylimidazole 2a or 4-methylimidazole 2b).

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Figure 2: Melting curves based on normalized UV absorbance at 260 nm of a) duplex I with X = 4-methylimidazol...

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Figure 3: CD spectra of a) duplex I with X = 4-methylimidazole and b) duplex II with X = 2-methylimidazole in...

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Published 09 Sep 2014

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