Synthetic incorporation of Nile Blue into DNA using 2′-deoxyriboside substitutes: Representative comparison of (R)- and (S)-aminopropanediol as an acyclic linker

• Daniel Lachmann,
• Sina Berndl,
• Otto S. Wolfbeis and
• Hans-Achim Wagenknecht

Beilstein J. Org. Chem. 2010, 6, No. 13, doi:10.3762/bjoc.6.13

• this chromophore to DNA. We interpret the similarity of the absorption properties together with the results from the thermal dehybridization studies, as discussed above, as a result of the intercalation of the Nile Blue dye in duplex DNA. To further explore the optical properties we recorded steady

• chains and the triazolyl group represents the most critical issue for intercalation. From both molecular models it became obvious that this tether is long and flexible enough allowing the Nile Blue chromophore to intercalate in a nearly perfectly stacked position between the adjacent base pairs. This