An uracil-linked hydroxyflavone probe for the recognition of ATP

• Márton Bojtár,
• Péter Zoltán Janzsó-Berend,
• Dávid Mester,
• Dóra Hessz,
• Mihály Kállay,
• Miklós Kubinyi and
• István Bitter

Beilstein J. Org. Chem. 2018, 14, 747–755, doi:10.3762/bjoc.14.63

• . Conclusions: The first, uracil-containing fluorescent ATP probe based on a hydroxyflavone fluorophore was synthesized and evaluated. A selective complexation with ATP was observed and a ratiometric response in the excitation spectrum. Keywords: ATP sensing; base-pairing; fluorescent probes; 3-hydroxyflavone

• processes of ATP [34]. The ideal ATP probe possessing all the prerequisites to bind ATP should be sensitive to electrostatic fields in solution and in molecular assemblies as well. 3-Hydroxyflavone (HF) fluorophores, especially the highly polarizable 4’-dialkylamino subfamily exhibit strong sensitivity to

• UHF which might be the result of the complementary nucleobase attached to the flavone scaffold. Ratiometric fluorescence measurements received particular attention in the past decade due to their high sensitivity and reliability by their inherent self-calibration nature [62]. Most 3-hydroxyflavone