Flow synthesis of phenylserine using threonine aldolase immobilized on Eupergit support

• Jagdish D. Tibhe,
• Hui Fu,
• Timothy Noël,
• Qi Wang,
• Jan Meuldijk and
• Volker Hessel

Beilstein J. Org. Chem. 2013, 9, 2168–2179, doi:10.3762/bjoc.9.254

• aldolase (TA) from Thermotoga maritima was immobilized on an Eupergit support by both a direct and an indirect method. The incubation time for the direct immobilization method was optimized for the highest amount of enzyme on the support. By introducing the immobilized TA in a packed-bed microreactor, a

• was achieved at 20 minute residence time. Finally, the productivity of the reactor was calculated, extrapolated to parallel run units, and compared with data collected previously. Keywords: Eupergit; flow chemistry; immobilized enzyme; threonine aldolase; Introduction Enzymes are bio-based catalysts

• as low diastereoselectivity and product yield. To overcome this drawback of aldolases, dynamic kinetic asymmetric transformation has been carried out in which a bi-enzymatic process was performed to achieve a high yield of the product by shifting the reaction equilibrium [45]. Eupergit oxirane