Beilstein J. Org. Chem.2019,15, 2644–2654, doi:10.3762/bjoc.15.257
-soluble and selective bioprobe for plasma membrane imaging. The new compound was efficiently synthesized in a two-step procedure with good yields. The photophysical properties were evaluated and the dye proved to have an excellent photostability in several solvents. DFT calculations were found in
agreement with the experimental data and helped to understand the stabilizing intramolecular charge-transfer process from the first excited state. The new fluorescent derivative could be applied as selective bioprobe in several cell lines and displayed plasma-membrane affinity during the imaging experiments
for all tested models.
Keywords: benzothiadiazole; bioprobe; cell imaging; fluorescence; mitochondria; molecular architecture; plasma membrane; Introduction
The selective staining of plasma membranes is of paramount importance to study cellular processes and events associated with this dynamic
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Graphical Abstract
Figure 1:
The 2,1,3-benzothiadiazole (BTD) core and its derivatives that are successfully applied in bioimagi...