Beilstein J. Org. Chem.2021,17, 1313–1322, doi:10.3762/bjoc.17.91
spectrometry (AP-SMALDI-HRMS), low-molecular-weight polar compounds were identified by comparative metabolomics in the chemosphere of Ulva. Choline (2-hydroxy-N,N,N-trimethylethan-1-aminium) was only determined in the alga grown under axenic conditions, whereas ectoine (1,4,5,6-tetrahydro-2-methyl-4
-pyrimidinecarboxylic acid) was found in bacterial presence. Ectoine was used as a metabolic marker for localisation studies of Roseovarius sp. within the tripartite community because it was produced exclusively by these bacteria. By combining confocal laser scanning microscopy (cLSM) and AP-SMALDI-HRMS, we proved that
distributions of metabolites and identifying specific symbiotic bacteria.
Keywords: algae; AP-SMALDI; ectoine; holobiont; high-resolution mass spectrometry; mass spectrometry imaging; marine bacteria; Ulva; Introduction
In intertidal zones with high temporal and spatial ecosystem variations, bacteria and
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Graphical Abstract
Figure 1:
Untargeted comparative metabolomics using AP-SMALDI-HRMS highlighted metabolites involved in Ulva–b...
Beilstein J. Org. Chem.2016,12, 969–984, doi:10.3762/bjoc.12.96
osmoprotective strategies of Enhygromyxa salina SWB007 and Plesiocystis pacifica SIR-1 revealed that both closely related strains rely on organic osmolytes. For instance, E. salina SWB007 biosynthesizes the osmolytes betaine, ectoine, and especially hydroxyectoine under high salt concentrations. In contrast, P
osmolytes like betaine and ectoine were detected in the halotolerant bacterium.
Taxonomy, cultivation and secondary metabolite chemistry of marine-derived myxobacteria (Nannocystineae)
The following sections summarize features of three halotolerant and three marine-derived bacterial taxa clustered in the
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Graphical Abstract
Figure 1:
Structures of cystobactamids 507, 919-1 and 919-2.