Clustering and curation of electropherograms: an efficient method for analyzing large cohorts of capillary electrophoresis glycomic profiles for bioprocessing operations

• Ian Walsh,
• Matthew S. F. Choo,
• Sim Lyn Chiin,
• Amelia Mak,
• Shi Jie Tay,
• Pauline M. Rudd,
• Yang Yuansheng,
• Andre Choo,
• Ho Ying Swan and
• Terry Nguyen-Khuong

Beilstein J. Org. Chem. 2020, 16, 2087–2099, doi:10.3762/bjoc.16.176

• ; electropherogram; glycosylation; monoclonal antibodies; peak picking; process development; Introduction Glycosylation is important for the efficacy and function of a majority of the most dominant biologic drugs currently on the global market. In the case of antibody-based biotherapeutics, the absence of

• different parameter settings for each electropherogram (or groups of similar electropherograms) requiring substantial human intervention to check correctness of the automated picked peaks and tuning parameters. This level of human manual data analysis is impractical when dealing with thousands of samples

• /FA2G2S1/A2 and M5 peaks where their relative sizes on the electropherogram compared well with the Riemann sum peak area calculations, i.e, almost equal areas (Figure 2A vs 2D), FA1/FA2G2S1/A2>M5 (Figure 2B vs 2E), and FA1/FA2G2S1/A2

Sequence-specific RNA cleavage by PNA conjugates of the metal-free artificial ribonuclease tris(2-aminobenzimidazole)

• Friederike Danneberg,
• Alice Ghidini,
• Plamena Dogandzhiyski,
• Elisabeth Kalden,
• Roger Strömberg and
• Michael W. Göbel

Beilstein J. Org. Chem. 2015, 11, 493–498, doi:10.3762/bjoc.11.55

• with the 15mer-conjugate 13 and its cognate RNA 16. Instead, the electropherogram showed a shift of several signals including the substrate peak (lane d, compare to hydrolysis ladder of 16 in lane g), making it difficult to assign the signals to individual cleavage fragments. However, the position of

Synthesis of modified cyclic and acyclic dextrins and comparison of their complexation ability

• Kata Tuza,
• László Jicsinszky,
• Tamás Sohajda,
• István Puskás and
• Éva Fenyvesi

Beilstein J. Org. Chem. 2014, 10, 2836–2843, doi:10.3762/bjoc.10.301

• electropherogram, respectively). These preliminary results demonstrate that these derivatives have to be considered not only as possible complexation agents but also as potential chiral selectors in the future. Electropherograms of 2-hydroxypropyl derivatives and five guests are presented below (Figure 2

• , the associate migrates closer to the EOF. As in each electropherogram the mobility of the detected peak is the average of that of the free and complexed species weighted by the molar fraction, the more pronounced this mobility change is, the stronger interaction is suggested. This indicates that in