Beilstein J. Org. Chem.2015,11, 469–472, doi:10.3762/bjoc.11.52
synthesis; nucleic acids; nucleoside-5’-diphosphate; phosphorylation; Introduction
Nucleoside-5'-phosphates are key to many mechanistic studies and chemical biology applications [1][2][3]. Synthetic approaches towards nucleoside-5'-phosphates are well-established [4], however, the methods tend to be
problems [4][19]. In addition, excess pyrophosphate is usually employed in order to drive the kinetics of the displacement process, however, this excess material tends to co-elute with nucleoside-5’-diphosphate products during anion exchange chromatographic purifications.
We have recently reported the