Beilstein J. Org. Chem.2022,18, 1278–1288, doi:10.3762/bjoc.18.134
PPK2. They are structurally unrelated and use different catalytic mechanisms. PPK1 enzymes prefer the usage of adenosine 5'-triphosphate (ATP) for polyphosphate (polyP) synthesis while PPK2 enzymes favour the reverse reaction. With the emerging use of PPK enzymes in biosynthesis, a deeper understanding
concentrations of substrate, the different kinetic preferences of PPK1 and PPK2 can be observed. The implications of these results for the application of PPKs in chemical synthesis and as enzymes for ATP regeneration systems are discussed.
Keywords: ATP regeneration; biocatalyst; ePC-SAFT; polyp; PPK
; Introduction
Polyphosphate (polyP, Figure 1) is a linear polymer of up to thousands of phosphate residues connected by phosphate anhydride bonds. It serves as a phosphate storage molecule and plays a crucial role in biofilm formation and stress responses of cells [1]. So far polyP has been detected in every
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Graphical Abstract
Figure 1:
Polyphosphate, a ubiquitous phosphate storage molecule. Reported chain lengths range from three to ...