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Search for "ratiometric" in Full Text gives 11 result(s) in Beilstein Journal of Organic Chemistry.
Selected peptide-based fluorescent probes for biological applications
Beilstein J. Org. Chem. 2020, 16, 2971–2982, doi:10.3762/bjoc.16.247
- for biomolecule detection [27][28]. Being intrinsically modular, peptide scaffolds also allow the attachment of multiple fluorophores simultaneously [29]. It provides the opportunity of developing ratiometric fluorescent sensors from the interaction between multiple fluorophores (Figure 1B
- to perform FRET in vitro and in vivo, particularly in living cells [30]. FRET-based peptide probes are heavily used for ratiometric fluorescence detection of biomolecules. Pyrene-functionalized oligonucleotides and locked nucleic acids (LNAs) are considerably used for targeting nucleic acids [31
- ]. The attachment of pyrenes to the termini of a flexible peptide linker results in a ratiometric fluorescent probe (Figure 1C). Upon interaction with a biomolecule, the distance between the pyrenes changes, subsequently their monomer to excimer and vice versa fluorescence signal changes. However, the
Using multiple self-sorting for switching functions in discrete multicomponent systems
Beilstein J. Org. Chem. 2020, 16, 2831–2853, doi:10.3762/bjoc.16.233
- innovative catch–release system with multiple functions combined the ON/OFF-adjustment of silver(I) catalysis and fluorescence monitoring [59]. Actually, the ratiometric luminescence response allowed the exact monitoring of the catalytic activity. In the initial incomplete self-sorting (state SelfSORT-I
Arylisoquinoline-derived organoboron dyes with a triaryl skeleton show dual fluorescence
Beilstein J. Org. Chem. 2019, 15, 2612–2622, doi:10.3762/bjoc.15.254
- ratiometric response by the build-up of a hypsochromically shifted emission signal. Keywords: anions; dyes; fluorescence; laser-flash photolysis; organoboron; Introduction Boron-containing tri- and tetra-coordinated chromophores have attracted considerable interest due to their often peculiar and highly
- the verification of the effect of aromatic conjugation (naphthyl, anthryl, pyrenyl) and electron-donor strength (naphthyl versus 4-methoxynaphthyl) on the photophysical properties. Beside the observation of interesting dual emission properties for these dyes, some showed a pronounced ratiometric
- LW band is substituted by a strong blue-shifted emission. This leads to a clear ratiometric behavior and a large dynamic response. The blue-shifted emission for the fluoroboronate Lewis adduct is in accordance with the observations made for donor solvents such as tetrahydrofuran (see above). As for
Targeted photoswitchable imaging of intracellular glutathione by a photochromic glycosheet sensor
Beilstein J. Org. Chem. 2019, 15, 2380–2389, doi:10.3762/bjoc.15.230
- state of the cells, e.g., causing false-positive/negative results. Generally, strategies like designing ratiometric [4][5] or near-infrared [6][7][8] fluorescence sensors are applied to overcome this obstacle. Recently, a novel category of photochromic probes with light-controlled dynamic fluorescence
Mono- and bithiophene-substituted diarylethene photoswitches with emissive open or closed forms
Beilstein J. Org. Chem. 2019, 15, 2344–2354, doi:10.3762/bjoc.15.227
- Table 1) is only weakly fluorescent (φFl ≈ 0.01) [4]. In general, diarylethenes with fluorescent open forms are rare, while photoswitchable compounds with highly fluorescent “open” and “closed” forms represent a yet unknown and unique class of ratiometric fluorophores. Recently, we demonstrated that
- nonemissive. This important property will be taken into account in the design of the future photoswitchable compounds with ratiometric fluorescence modulation. Switching kinetics Table 2 contains an overview of the most important parameters related to the switching kinetics. All compounds, except AsOTh2 and
An overview of recent advances in duplex DNA recognition by small molecules
Beilstein J. Org. Chem. 2018, 14, 1051–1086, doi:10.3762/bjoc.14.93
- and its cytotoxicity was attributed to DNA minor groove binding ability [105]. Recently, Schmuck et al. have developed a first prototype of cationic oligopeptide-based molecular beacon (conjugate 43) coupled with a FRET pair, a naphthalene donor and a dansyl acceptor, for ratiometric detection of ds
An uracil-linked hydroxyflavone probe for the recognition of ATP
Beilstein J. Org. Chem. 2018, 14, 747–755, doi:10.3762/bjoc.14.63
- . Conclusions: The first, uracil-containing fluorescent ATP probe based on a hydroxyflavone fluorophore was synthesized and evaluated. A selective complexation with ATP was observed and a ratiometric response in the excitation spectrum. Keywords: ATP sensing; base-pairing; fluorescent probes; 3-hydroxyflavone
- electric fields generated by ions and molecules in solution. This property along the ESIPT process (excited state intramolecular proton transfer) [35] makes them ideal for ratiometric environment-sensitive probes and sensors [36][37][38][39][40][41][42][43]. Among them the 4’-dimethylamino derivative (DMHF
- ratiometric fluorescent nature. First, we examined the possible structure and the supramolecular interactions by quantum chemical calculations of our target compound, UHF (uracil-hydroxyflavone) and ATP (see Figure 1 for structures). The theoretical results indicated the possibility of base-pairing
New synthetic strategies for xanthene-dye-appended cyclodextrins
Beilstein J. Org. Chem. 2016, 12, 537–548, doi:10.3762/bjoc.12.53
- agents for the carboxylic acid were HOBt and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). Fang and co-workers designed a ratiometric sensor for detecting mercury ions in aqueous media, some biological fluids and living cells based on a rhodamine-modified β-CD [16]. The synthetic strategy was a
Design, synthesis and photochemical properties of the first examples of iminosugar clusters based on fluorescent cores
Beilstein J. Org. Chem. 2015, 11, 659–667, doi:10.3762/bjoc.11.74
- adequate tailoring, including fluorescence amplification [50], and ratiometric pH reporter for imaging protein–dye conjugates in living cells [51], or display physiological binding of D-glucose [52]. The pyrene nucleus was also selected as an alternative fluorophore since it may be easily
A simple and efficient dual optical signaling chemodosimeter for toxic Hg(II)
Beilstein J. Org. Chem. 2012, 8, 1352–1357, doi:10.3762/bjoc.8.155
- ratiometric or switch-on responses even for the paramagnetic metal ions [17][18][19]. The first luminescence chemodosimeter for Hg2+ was developed by Czarnik et al. and was based on the Hg2+-mediated desulfurization of anthracene-thioamide chromophore [20][21]. Following this pioneering report, several other
Ratiometric fluorescent probe for enantioselective detection of D-cysteine in aqueous solution
Beilstein J. Org. Chem. 2011, 7, 1508–1515, doi:10.3762/bjoc.7.176
- Xiao-bo Zhou Wing-Hong Chan Albert W. M. Lee Chi-Chung Yeung Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China 10.3762/bjoc.7.176 Abstract A ratiometric fluorescent probe based on a Cd2+–ACAQ complex was designed and demonstrated for the chemo- and
- ; fluorescence; ratiometric; Introduction The rapid, sensitive, and selective sensing of biologically relevant thiols including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) has attracted significant interest in the sensor community (Figure 1) [1]. All three of these thiols are present in living
- documented [22][23][24][25][26][27][28]. As an improved sensing device, a ratiometric fluorescence probe monitors the emission intensities at two different wavelengths, which should provide a built-in correction for instrumental defects and photodegradation of sensing materials. In this context, a few
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