Beilstein J. Org. Chem.2020,16, 2607–2622, doi:10.3762/bjoc.16.212
substrates of the phosphorolysis of uridine, thymidine, and 1-(β-ᴅ-arabinofuranosyl)uracil (Ara-U) catalyzed by recombinant E. coli uridine (UP) and thymidine (TP) phosphorylases. α-ᴅ-Pentofuranose-1-phosphates (PF-1Pis) obtained by phosphorolysis were used in the enzymatic synthesis of nucleosides. It was
were tested in the synthesis of nelarabine, kinetin riboside, and cladribine with good to excellent yields (52–93%).
Keywords: anion exchange resins; N6-benzyladenosine; cladribine; enzymatic glycosylation; kinetin riboside; nelarabine; α-ᴅ-pentofuranose-1-phosphates; phosphorolysis of nucleosides
][3][4][5][6][7][8][9][10][11][12][13][14][15]). In the late forties and early fifties of the last century, α-ᴅ-pentofuranose-1-phosphates (PF-1Pis) were isolated from the phosphorolysis of natural nucleosides catalyzed by nucleoside phosphorylases and their structures and enzymatic reactions with
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Graphical Abstract
Scheme 1:
General scheme of the suggested synthesis of nucleosides employing the enzymatic phosphorolysis of ...