Supporting Information
Supporting Information features detailed information on the synthesis of magnetic iron oxide nanoparticles, electrode fabrication, and sample preparation. Also, the characterization of MNPs and electrode surfaces by using Fourier-transform infrared spectroscopy (FTIR), field-emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS, Figure S1) is described. The Silhouette coefficients calculated for IDMAP, Sammon’s mapping (SM), principal component analysis (PCA), and least square projection (LSP) multidimensional projection techniques to analyze the PSA concentration of immunosensor before (All) and after (FS) feature selection (Figure S2) are provided as well. The parallel coordinates plot for PSA concentrations from 12.5 to 1111 fg·mL−1 are given in Figure S3, while plots of three multidimensional projection techniques, i.e., PCA, LSP, and SM are shown in Figure S4.
| Supporting Information File 1: Additional procedures and figures. | ||
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