Nanolesions induced by heavy ions in human tissues: Experimental and theoretical studies

Marcus Bleicher, Lucas Burigo, Marco Durante, Maren Herrlitz, Michael Krämer, Igor Mishustin, Iris Müller, Francesco Natale, Igor Pshenichnov, Stefan Schramm, Gisela Taucher-Scholz and Cathrin Wälzlein

Beilstein J. Nanotechnol. 2012, 3, 556–563. https://doi.org/10.3762/bjnano.3.64

Supporting Information

Supporting Information File 1: The animation in Supporting Information File 1 shows a real time observation of the recruitment of GFP-XRCC1 to two charged particle tracks traversing the nucleus of a living MEF cell during high energy (1 GeV/n) uranium irradiation. From these 3-D image stacks, movies were generated by making maximum projections of the fluorescence intensity using Image J (http://rsb.info.nih.gov/ij/). Red color indicates Cherry-tagged HP1α (marking chromocenters), green color GFP-XRCC1. Total imaging time: 9.5 min. Shot noise (due to neutron scattering) indicates the irradiation time points. Please note the fast GFP-XRCC1 recruitment along tracks, disappearance of euchromatic foci (green) and the prolonged retention of heterochromatic GFP-XRCC1 (yellow, overlapping HP1α) in the left radiation track.
Format: AVI	Size: 384.4 KB	Download
Supporting Information File 2: Supporting Information File 2 is a high resolution animation showing real time GFP-XRCC1 recruitment to the high energy uranium ion track traversing a single MEF chromocenter (red, marked by Cherry-HP1α). Note the billowing motion of the damaged domain (XRCC1, green; appears yellow due to HP1α overlap in heterochromatin) and a drift toward the chromocenter periphery.
Format: AVI	Size: 504.2 KB	Download

Cite the Following Article

Nanolesions induced by heavy ions in human tissues: Experimental and theoretical studies

Beilstein J. Nanotechnol. 2012, 3, 556–563. https://doi.org/10.3762/bjnano.3.64

How to Cite

Bleicher, M.; Burigo, L.; Durante, M.; Herrlitz, M.; Krämer, M.; Mishustin, I.; Müller, I.; Natale, F.; Pshenichnov, I.; Schramm, S.; Taucher-Scholz, G.; Wälzlein, C. Beilstein J. Nanotechnol. 2012, 3, 556–563. doi:10.3762/bjnano.3.64

Download Citation

Citation data can be downloaded as file using the "Download" button or used for copy/paste from the text window below.
Citation data in RIS format can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Zotero.

File format:

RIS

BibTeX

Include:

Citation for the article above

Citation and complete reference list for the article above

Download

TY  - JOUR
A1  - Bleicher, Marcus
A1  - Burigo, Lucas
A1  - Durante, Marco
A1  - Herrlitz, Maren
A1  - Krämer, Michael
A1  - Mishustin, Igor
A1  - Müller, Iris
A1  - Natale, Francesco
A1  - Pshenichnov, Igor
A1  - Schramm, Stefan
A1  - Taucher-Scholz, Gisela
A1  - Wälzlein, Cathrin
T1  - Nanolesions induced by heavy ions in human tissues: Experimental and theoretical studies
JF  - Beilstein Journal of Nanotechnology
PY  - 2012///
VL  - 3
SP  - 556
EP  - 563
SN  - 2190-4286
DO  - 10.3762/bjnano.3.64
PB  - Beilstein-Institut
JA  - Beilstein J. Nanotechnol.
UR  - https://doi.org/10.3762/bjnano.3.64
KW  - DNA repair
KW  - heavy ions
KW  - microdosimetry
KW  - Monte Carlo simulations
KW  - nanolesions
KW  - radiation-induced nanostructures
N2  - The biological effects of energetic heavy ions are attracting increasing interest for their applications in cancer therapy and protection against space radiation. The cascade of events leading to cell death or late effects starts from stochastic energy deposition on the nanometer scale and the corresponding lesions in biological molecules, primarily DNA. We have developed experimental techniques to visualize DNA nanolesions induced by heavy ions. Nanolesions appear in cells as “streaks” which can be visualized by using different DNA repair markers. We have studied the kinetics of repair of these “streaks” also with respect to the chromatin conformation. Initial steps in the modeling of the energy deposition patterns at the micrometer and nanometer scale were made with MCHIT and TRAX models, respectively.
ER  -

Citations to This Article

Up to 20 of the most recent references are displayed here.

Scholarly Works

Di Natale, F.; Vivo, M.; Falco, G.; Angrisano, T. Deciphering DNA methylation signatures of pancreatic cancer and pancreatitis. Clinical epigenetics 2019, 11, 1–12. doi:10.1186/s13148-019-0728-8
Fontana, R.; Ranieri, M.; La Mantia, G.; Vivo, M. Dual Role of the Alternative Reading Frame ARF Protein in Cancer. Biomolecules 2019, 9, 87–108. doi:10.3390/biom9030087
de Vera, P.; Surdutovich, E.; Abril, I.; Garcia-Molina, R.; Solov’yov, A. V. Analytical model of ionization and energy deposition by proton beams in subcellular compartments. The European Physical Journal D 2014, 68, 96. doi:10.1140/epjd/e2014-50041-7
Falk, M.; Hausmann, M.; Lukášová, E.; Biswas, A.; Hildenbrand, G.; Davídková, M.; Krasavin, E.; Kleibl, Z.; Falková, I.; Ježková, L.; Štefančíková, L.; Sevcik, J.; Hofer, M.; Bačíková, A.; Matula, P.; Boreyko, A.; Vachelová, J.; Michaelidesová, A.; Kozubek, S. Determining Omics spatiotemporal dimensions using exciting new nanoscopy techniques to assess complex cell responses to DNA damage: part A--radiomics. Critical reviews in eukaryotic gene expression 2014, 24, 205–223. doi:10.1615/critreveukaryotgeneexpr.2014010313
Burigo, L.; Pshenichnov, I.; Mishustin, I.; Bleicher, M. Microdosimetry of radiation field from a therapeutic 12C beam in water: A study with Geant4 toolkit. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 2013, 310, 37–53. doi:10.1016/j.nimb.2013.05.021
Falk, M.; Lukášová, E.; Štefančíková, L.; Baranova, E.; Falková, I.; Ježková, L.; Davídková, M.; Bačíková, A.; Vachelová, J.; Michaelidesová, A.; Kozubek, S. Heterochromatinization associated with cell differentiation as a model to study DNA double strand break induction and repair in the context of higher-order chromatin structure. Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine 2013, 83, 177–185. doi:10.1016/j.apradiso.2013.01.029

Explore citations to this article at

Back To Article

Other Beilstein-Institut Open Science Activities

aromatic	the word “aromatic”
aromatic aldehyde	the word “aromatic” OR “aldehyde”
+aromatic +aldehyde	both words “aromatic” AND “aldehyde”
+aromatic -aldehyde	the word “aromatic” but NOT “aldehyde”
“aromatic aldehyde”	the exact phrase “aromatic aldehyde”
benz*	words which begin with “benz”, such as “benzene” or “benzyl”
benz*yl	words that begin with “benz” and end with “yl”, such as “benzyl” or “benzoyl”
benzyl~	words that are close to the word “benzyl”, such as “benzoyl” (i.e., fuzzy search)