Optical bio/chemical sensors for vitamin B₁₂ analysis in food and pharmaceuticals: state of the art, challenges, and future outlooks

• Seyed Mohammad Taghi Gharibzahedi and
• Zeynep Altintas

Beilstein J. Nanotechnol. 2025, 16, 2207–2244, doi:10.3762/bjnano.16.153

Rapid synthesis of highly monodisperse AgSbS₂ nanocrystals: unveiling multifaceted activities in cancer therapy, antibacterial strategies, and antioxidant defense

• Funda Ulusu,
• Adem Sarilmaz,
• Yakup Ulusu,
• Faruk Ozel and
• Mahmut Kus

Beilstein J. Nanotechnol. 2025, 16, 2105–2115, doi:10.3762/bjnano.16.145

• inset in Figure 2c. The particle size distribution graphs in this image unequivocally affirmed the successful attainment of the desired homogeneity. Biological applications Antibacterial activity of AgSbS2 NCs Disc diffusion. The antimicrobial activity of AgSbS2 NCs at a concentration of 10 mg/mL

On the road to sustainability – application of metallic nanoparticles obtained by green synthesis in dentistry: a scoping review

• Lorena Pinheiro Vasconcelos Silva,
• Joice Catiane Soares Martins,
• Israel Luís Carvalho Diniz,
• Júlio Abreu Miranda,
• Danilo Rodrigues de Souza,
• Éverton do Nascimento Alencar,
• Moan Jéfter Fernandes Costa and
• Pedro Henrique Sette-de-Souza

Beilstein J. Nanotechnol. 2025, 16, 1851–1862, doi:10.3762/bjnano.16.128

• , including cements, sealants, and mouthwashes [8][45]. CuNPs and nickel oxide nanoparticles, though less frequent, are also gaining attention for their antimicrobial activity, despite some concerns regarding cytotoxicity [46][47]. The presence AuNPs and titanium dioxide nanoparticles highlights an interest

• investigation of antimicrobial activity against these microorganisms indicates the potential dental applications of green-synthesized metallic nanoparticles. To characterize the green-synthesized metallic nanoparticles, the selected studies used mainly Fourier-transform infrared spectroscopy (FTIR, 36.73%; n

• , particularly in dentistry. This scoping review examines the rising focus on these nanoparticles regarding their antimicrobial, regenerative, and therapeutic potential in dental applications. Among the metals studied, silver and zinc oxide nanoparticles dominate because of their broad-spectrum antimicrobial

Synthesis and antibacterial properties of nanosilver-modified cellulose triacetate membranes for seawater desalination

• Lei Wang,
• Shizhe Li,
• Kexin Xu,
• Wenjun Li,
• Ying Li and
• Gang Liu

Beilstein J. Nanotechnol. 2025, 16, 1380–1391, doi:10.3762/bjnano.16.100

• nanomaterials can be engineered to optimize their antimicrobial activity, making them versatile tools in the development of advanced membrane technologies. Quantum effects, such as localized surface plasmon resonance in metallic nanoparticles, can further enhance antimicrobial properties by generating reactive

Enhancing the therapeutical potential of metalloantibiotics using nano-based delivery systems

• Alejandro Llamedo,
• Marina Cano,
• Raquel G. Soengas and
• Francisco J. García-Alonso

Beilstein J. Nanotechnol. 2025, 16, 1350–1366, doi:10.3762/bjnano.16.98

• antimicrobial agents since they usually exhibit superior antimicrobial activity compared to silver(I) alone [100]. The mechanism of action of most silver complexes is based on a slow release of the silver(I) ions, which react with the thiol groups of proteins or with key functional groups of enzymes; the

• cellulose-based dressings. The resulting formulation displayed improved antimicrobial activity and enhanced wound healing properties [105]. Additionally, in vitro assays revealed that encapsulating silver sulfadiazine in SLNs protected fibroblasts and keratinocytes from metal-induced toxicity. Finally, the

• , and the resulting formulation was evaluated regarding its antimicrobial activity against Gram-positive bacteria (e.g., S. aureus and B. subtilis) and Gram-negative strains (e.g., E. coli and P. aeruginosa) [121]. Unfortunately, encapsulation led to reduced immediate efficacy compared to the free Zn

Functional bio-packaging enhanced with nanocellulose from rice straw and cinnamon essential oil Pickering emulsion for fruit preservation

• Tuyen B. Ly,
• Duong D. T. Nguyen,
• Hieu D. Nguyen,
• Yen T. H. Nguyen,
• Bup T. A. Bui,
• Kien A. Le and
• Phung K. Le

Beilstein J. Nanotechnol. 2025, 16, 1234–1245, doi:10.3762/bjnano.16.91

• 600 nm and its thickness as (Equation 4). where Ab600 represents the absorbance at 600 nm and t is the biopackaging thickness [49]. Antimicrobial properties. The antimicrobial activity of the films was evaluated using a time-kill method. Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213

• (CFU) were counted to determine the antimicrobial activity of the films. Inhibition rates were calculated according to where CCcontrol is the cell count of the control tube substance and CCsample is the cell count of the sample after 24 h. Antioxidation properties. The antioxidant capacity of the

Hydrogels and nanogels: effectiveness in dermal applications

• Jéssica da Cruz Ludwig,
• Diana Fortkamp Grigoletto,
• Daniele Fernanda Renzi,
• Wolf-Rainer Abraham,
• Daniel de Paula and
• Najeh Maissar Khalil

Beilstein J. Nanotechnol. 2025, 16, 1216–1233, doi:10.3762/bjnano.16.90

• through the oxidative coupling of catechol groups using a hydrogen peroxide/horseradish peroxidase (H2O2/HRP) catalytic system [41]. The addition of the antibiotic doxycycline provided the hydrogels with antimicrobial activity to treat infected full-thickness defect wounds. Flexible films composed of

• the extracellular matrix make them attractive drug delivery systems. Dermal applications include psoriasis treatment, antimicrobial activity, wound healing, and skin tumor remission. Understanding topical and transdermal drug delivery mechanisms is crucial. Hydrogels and nanogels, proven strategies to

Quantification of lead through rod-shaped silver-doped zinc oxide nanoparticles using an electrochemical approach

• Ravinder Lamba,
• Gaurav Bhanjana,
• Neeraj Dilbaghi,
• Vivek Gupta and
• Sandeep Kumar

Beilstein J. Nanotechnol. 2025, 16, 422–434, doi:10.3762/bjnano.16.33

• , India Department of Physics, Punjab Engineering College (Deemed to be University), Chandigarh, 160012, India 10.3762/bjnano.16.33 Abstract Special features of zinc oxide nanoparticles have drawn a lot of interest due to their wide bandgap, high surface area, photocatalytic activity, antimicrobial

• activity, and semiconductor properties. By doping ZnO nanoparticles with transition metals, we can alter their electrical, optical, and magnetic properties by introducing new electronic states into the band structure. Herein, Ag is added to ZnO nanostructures to improve their optical properties to detect

Fabrication and evaluation of BerNPs regarding the growth and development of Streptococcus mutans

• Tuyen Huu Nguyen,
• Hong Thanh Pham,
• Kieu Kim Thanh Nguyen,
• Loan Hong Ngo,
• Anh Ngoc Tuan Mai,
• Thu Hoang Anh Lam,
• Ngan Thi Kim Phan,
• Dung Tien Pham,
• Duong Thuy Hoang,
• Thuc Dong Nguyen and
• Lien Thi Xuan Truong

Beilstein J. Nanotechnol. 2025, 16, 308–315, doi:10.3762/bjnano.16.23

• -transform infrared spectroscopy. The broth dilution method was used to determine the antimicrobial activity of the BerNPs against Streptococcus mutans (S. mutans). The impact of the BerNPs on the cell surface of S. mutans was evaluated through FE-SEM analysis, focusing on its ability to inhibit biofilm

• is currently little information on its antimicrobial activity against S. mutans. Therefore, it is scientifically and economically important to clarify the mechanism and ability of BerNPs to inhibit the growth of S. mutans. The findings from such studies could serve as a foundation for developing

Enhancing mechanical properties of chitosan/PVA electrospun nanofibers: a comprehensive review

• Nur Areisman Mohd Salleh,
• Amalina Muhammad Afifi,
• Fathiah Mohamed Zuki and
• Hanna Sofia SalehHudin

Beilstein J. Nanotechnol. 2025, 16, 286–307, doi:10.3762/bjnano.16.22

• improved the antimicrobial activity of the nanofibers against a wide range of bacteria [190]. In tissue engineering applications, aligned fibers are particularly effective as they better mimic the inductive environment, such as that of human tendon stem/progenitor cells, compared to random fibers [191

Clays enhanced with niobium: potential in wastewater treatment and reuse as pigment with antibacterial activity

• Silvia Jaerger,
• Patricia Appelt,
• Mario Antônio Alves da Cunha,
• Fabián Ccahuana Ayma,
• Ricardo Schneider,
• Carla Bittencourt and
• Fauze Jacó Anaissi

Beilstein J. Nanotechnol. 2025, 16, 141–154, doi:10.3762/bjnano.16.13

• characterized through colorimetric analysis (CIEL*a*b*) and UV–vis spectroscopy. Antimicrobial activity test The antimicrobial properties of the BEPh, BEOx, A-BEPh, A-BEOx, A-BEPhP, and A-BEOxP samples were investigated against the bacteria Bacillus cereus (ATCC 10876) (Gram-positive) and Proteus mirabilis

Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258

• Prashantkumar Siddappa Chakra,
• Aishwarya Banakar,
• Shriram Narayan Puranik,
• Vishwas Kaveeshwar,
• C. R. Ravikumar and
• Devaraja Gayathri

Beilstein J. Nanotechnol. 2025, 16, 78–89, doi:10.3762/bjnano.16.8

• semiconductors. Also, ZnO NPs exhibit antimicrobial activity, targeted drug delivery, catalytic activity, and antidiabetic, larvicidal, acaricidal and anticancer activity in addition to their usage in different medical devices and pharmaceuticals [11][12][13]. We report the ecologically safe production of ZnO

Natural nanofibers embedded in the seed mucilage envelope: composite hydrogels with specific adhesive and frictional properties

• Agnieszka Kreitschitz and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2024, 15, 1603–1618, doi:10.3762/bjnano.15.126

• friction better fulfil their biological role than mucilaginous seeds with just low friction, which was demonstrated in our experiment with pigeons (see below). Friction after chemical and thermal treatments The antimicrobial activity of seed mucilage was previously described for Linum usitatissimum [105

• as food, agriculture, and medicine [20][114][115]. Diverse other substances and systems with antimicrobial activity (antibiotics, essential oils, phenolics, and metal nanoparticles) can be incorporated into the mucilage hydrogels, which after some additional modifications can be used for the

Nanotechnological approaches in the treatment of schistosomiasis: an overview

• Lucas Carvalho,
• Michelle Sarcinelli and
• Beatriz Patrício

Beilstein J. Nanotechnol. 2024, 15, 13–25, doi:10.3762/bjnano.15.2

• drugs were able to cause alterations in parasite tegument [64]. Another work used carvacrol, a monoterpene present in essential oils derived from plants such as Origanum vulgare. Besides being commonly used as a flavoring agent in food and cosmetics, it shows antimicrobial activity. Xavier et al. [67

• mechanism by which the nanoemulsion could reduce the worm burden is its antimicrobial activity, connecting changes in microbiota with the response to parasites. However, the mechanism of action of carvacrol remains unknown [67]. Repositioned drugs Works utilizing compounds repositioned from other diseases

Quercetin- and caffeic acid-functionalized chitosan-capped colloidal silver nanoparticles: one-pot synthesis, characterization, and anticancer and antibacterial activities

• Akif Hakan Kurt,
• Elif Berna Olutas,
• Fatma Avcioglu,
• Hamza Karakuş,
• Mehmet Ali Sungur,
• Cansu Kara Oztabag and
• Muhammet Yıldırım

Beilstein J. Nanotechnol. 2023, 14, 362–376, doi:10.3762/bjnano.14.31

• as crabs, and shrimps. It is also found in the skeleton of insects and the structure of cell walls of fungi [11][12]. Chitosan, which was defined as an antimicrobial agent for the first time by Allan and Hadwiger, exhibits broad-spectrum antimicrobial activity [13][14]. In the last decade, chitosan

Antimicrobial and mechanical properties of functionalized textile by nanoarchitectured photoinduced Ag@polymer coating

• Jessica Plé,
• Marine Dabert,
• Helene Lecoq,
• Sophie Hellé,
• Lydie Ploux and
• Lavinia Balan

Beilstein J. Nanotechnol. 2023, 14, 95–109, doi:10.3762/bjnano.14.11

• forward. In the present study, the antimicrobial activity of E. coli and C. albicans is only due to the action of silver ions released by AgNPs [20]. Ionic silver first perforates the cell wall, enters the microorganism and eventually induces cell apoptosis. Indeed, once inside the cell, silver interacts

• , the same medium was chosen for both bacteria and fungus. M63G medium has been preferred since it does not reduce silver antimicrobial activity contrary to media containing components such as Cl− or other biomolecules, which interact with ionic silver [20]. In addition, it provides nutrient conditions

• demonstrates lower antimicrobial activity than the liquid diffusion tests. This can be first explained by silver’s limited diffusion through agar, compared to liquid media. In addition, the sample surface contribution is limited to its immediate vicinity in the case of the plate diffusion test, whereas the

In search of cytotoxic selectivity on cancer cells with biogenically synthesized Ag/AgCl nanoparticles

• Mitzi J. Ramírez-Hernández,
• Mario Valera-Zaragoza,
• Omar Viñas-Bravo,
• Ariana A. Huerta-Heredia,
• Miguel A. Peña-Rico,
• Erick A. Juarez-Arellano,
• David Paniagua-Vega,
• Eduardo Ramírez-Vargas and
• Saúl Sánchez-Valdes

Beilstein J. Nanotechnol. 2022, 13, 1505–1519, doi:10.3762/bjnano.13.124

• and anticancer properties of AgNPs synthesized from pineapple peel. The authors reported a favorable antimicrobial activity at low concentrations of AgNPs. Das et al. [17] found that AgNPs synthesized in the same way have high antidiabetic potential and high cytotoxicity against HepG2 cancer cells in

Hydroxyapatite–bioglass nanocomposites: Structural, mechanical, and biological aspects

• Olga Shikimaka,
• Mihaela Bivol,
• Bogdan A. Sava,
• Marius Dumitru,
• Christu Tardei,
• Beatrice G. Sbarcea,
• Daria Grabco,
• Constantin Pyrtsac,
• Daria Topal,
• Andrian Prisacaru,
• Vitalie Cobzac and
• Viorel Nacu

Beilstein J. Nanotechnol. 2022, 13, 1490–1504, doi:10.3762/bjnano.13.123

• addition of different dopants to the glass can enhance the osteogenesis (Mg, Sr, Fe, and Zr) or can yield particular properties such as angiogenesis (Cu and Co) and antimicrobial activity (Ag, Zn, Ce, Ga, and Cu). Also, dopants can change the glass solubility [13][15][16][17][18][19]. The presence of the

Supramolecular assembly of pentamidine and polymeric cyclodextrin bimetallic core–shell nanoarchitectures

• Alexandru-Milentie Hada,
• Nina Burduja,
• Marco Abbate,
• Claudio Stagno,
• Guy Caljon,
• Louis Maes,
• Nicola Micale,
• Massimiliano Cordaro,
• Angela Scala,
• Antonino Mazzaglia and
• Anna Piperno

Beilstein J. Nanotechnol. 2022, 13, 1361–1369, doi:10.3762/bjnano.13.112

• ) with pentamidine (Pent), an antimicrobial agent used against leishmaniasis [16], to investigate the antimicrobial activity of novel nanosystems (i.e., nanoGSP; Figure 1). Leishmaniasis is an infectious disease caused by protozoan parasites transmitted to humans and animals by the bite of tiny (2–3 mm

Biomimetic chitosan with biocomposite nanomaterials for bone tissue repair and regeneration

• Se-Kwon Kim,
• Sesha Subramanian Murugan,
• Pandurang Appana Dalavi,
• Sebanti Gupta,
• Sukumaran Anil,
• Gi Hun Seong and
• Jayachandran Venkatesan

Beilstein J. Nanotechnol. 2022, 13, 1051–1067, doi:10.3762/bjnano.13.92

• mechanical strength and cell adhesion. Importantly, the developed biocomposites have excellent antimicrobial activity [111]. AgNP-loaded fibrillar collagen–chitosan matrix was used for further biomineralisation using a simulated body fluid (SBF) solution. The developed composites show better mineralisation

• and also significant antimicrobial activity [112]. The hydroxyapatite was coated onto titanium by using an induction plasma-spray coating approach. The hydroxyapatite was then buffed with silver and silica. In the SBF solution, the release of acemannan from the coating material was also evaluated

• surface area, antimicrobial activity, mechanical strength, osteoconductive and osteoinductive properties. In this particular study, the scaffolds of chitosan/polyvinyl alcohol/graphene oxide/hydroxyapatite/gold films were used in an orthopaedic application. The mechanical strength of the developed

Bioselectivity of silk protein-based materials and their bio-inspired applications

• Hendrik Bargel,
• Vanessa T. Trossmann,
• Christoph Sommer and
• Thomas Scheibel

Beilstein J. Nanotechnol. 2022, 13, 902–921, doi:10.3762/bjnano.13.81

• through direct antimicrobial activity, (2) inhibiting biofilm-specific signalling pathways, and (3) modulating the innate immune responses resulting in the inhibition of potentially harmful inflammation reactions [85][86][88]. In order to highlight the multifaceted nature of AMPs, the term host defense

• spider silk proteins from Nephila clavipes with the AMPs human neutrophil defensin 2 and 4 (HNP-2, HNP-4) and hepcidin, showed antimicrobial

Ciprofloxacin-loaded dissolving polymeric microneedles as a potential therapeutic for the treatment of S. aureus skin infections

• Sharif Abdelghany,
• Walhan Alshaer,
• Yazan Al Thaher,
• Maram Al Fawares,
• Amal G. Al-Bakri,
• Saja Zuriekat and
• Randa SH. Mansour

Beilstein J. Nanotechnol. 2022, 13, 517–527, doi:10.3762/bjnano.13.43

• on agarose gel as an in vitro model of human skin showed that the formula was able to fully perforate the agarose gel. Moreover, this formula showed significantly greater antimicrobial activity (p < 0.0001) compared to a free gel of ciprofloxacin against Staphylococcus aureus in an agarose gel-based

• amount of ciprofloxacin was placed on top of the skin, followed by the stainless steel weight for consistency. Antimicrobial activity: in vitro infection study S. aureus (ATCC-29213) inoculum equivalent to 0.5 MacFarland (ca. 1 × 108 CFU/mL) turbidity was prepared from an overnight culture. A sterile

• frequently wetted with PBS to prevent the skin from drying out. At each predefined time point, CIP_MN1 was removed, and the skin was sliced using a cryostatic microtome. In vitro model for the antimicrobial activity of CIP_MN1. Preincubated inoculum of S. aureus is streaked over an agar plate. An artificial

Engineered titania nanomaterials in advanced clinical applications

• Padmavati Sahare,
• Paulina Govea Alvarez,
• Juan Manual Sanchez Yanez,
• Gabriel Luna-Bárcenas,
• Samik Chakraborty,
• Sujay Paul and
• Miriam Estevez

Beilstein J. Nanotechnol. 2022, 13, 201–218, doi:10.3762/bjnano.13.15

• photocatalytic reaction, as a result of which anatase has been found to have the highest antimicrobial activity among all crystal structures of TiO2 [79]. The mechanisms of titania-induced biocidal activity are mostly by an oxidative attack on the outer/inner cell membrane of the microorganism, as well as

• standard glass surface [82]. Another recent study stated that hollow, calcined TiO2 nanospheres (CSTiO2), synthesized by the combination of electrospinning and atomic layer deposition, have high antimicrobial activity against multidrug-resistant bacteria such as S. aureus strains compared to commercial

• , and it is considered a potent candidate for modifying TiO2 by photodeposition or radiolytic reduction. The prepared material exhibited antibacterial and antifungal properties under UV, visible and solar irradiation, and even in darkness [85]. Intriguingly, an enhanced antimicrobial activity of TiO2

A comprehensive review on electrospun nanohybrid membranes for wastewater treatment

• Senuri Kumarage,
• Imalka Munaweera and
• Nilwala Kottegoda

Beilstein J. Nanotechnol. 2022, 13, 137–159, doi:10.3762/bjnano.13.10

Sputtering onto liquids: a critical review

• Anastasiya Sergievskaya,
• Adrien Chauvin and
• Stephanos Konstantinidis

Beilstein J. Nanotechnol. 2022, 13, 10–53, doi:10.3762/bjnano.13.2