Self-assembly and adhesive properties of Pollicipes pollicipes barnacle cement protein cp19k: influence of pH and ionic strength

• Shrutika Sawant,
• Anne Marie Power and
• J. Gerard Wall

Beilstein J. Nanotechnol. 2025, 16, 1863–1872, doi:10.3762/bjnano.16.129

• of 12 nm. Spectra were plotted after subtracting the fluorescence values of the respective buffers. Negative controls (bovine serum albumin (BSA; lyophilised powder, Merck) and hen egg white lysozyme (Fluka)) and a positive control (hen egg white lysozyme denatured at 60 °C for 48 h in 0.1 M HCl-KCl

Exploring the potential of polymers: advancements in oral nanocarrier technology

• Rousilândia de Araujo Silva,
• Igor Eduardo Silva Arruda,
• Luise Lopes Chaves,
• Mônica Felts de La Roca Soares and
• Jose Lamartine Soares Sobrinho

Beilstein J. Nanotechnol. 2025, 16, 1751–1793, doi:10.3762/bjnano.16.122

Venom-loaded cationic-functionalized poly(lactic acid) nanoparticles for serum production against Tityus serrulatus scorpion

• Philippe de Castro Mesquita,
• Karla Samara Rocha Soares,
• Manoela Torres-Rêgo,
• Emanuell dos Santos-Silva,
• Mariana Farias Alves-Silva,
• Alianda Maira Cornélio,
• Matheus de Freitas Fernandes-Pedrosa and
• Arnóbio Antônio da Silva-Júnior

Beilstein J. Nanotechnol. 2025, 16, 1633–1643, doi:10.3762/bjnano.16.115

• % and 1.0% (w/w) with T. serrulatus venom was possible to evidence that all proteins of the venom were incorporated within the polymeric matrix, since the protein bands were not evidenced in the gel. The PLA nanoparticles and bovine serum albumin (BSA) were used as controls (Figure 1). This assay

• *** or ###p < 0.001, ** or ##p < 0.01, or * or #p < 0.05 were considered statistically significant. Electrophoretic profile of Tityus serrulatus venom (Tsv), bovine serum albumin (BSA), PLA nanoparticles (NPs), T. serrulatus venom protein-loaded PLA nanoparticles at 0.5% (NPs + Tsv 0.5%), and T

Better together: biomimetic nanomedicines for high performance tumor therapy

• Imran Shair Mohammad,
• Gizem Kursunluoglu,
• Anup Kumar Patel,
• Hafiz Muhammad Ishaq,
• Cansu Umran Tunc,
• Dilek Kanarya,
• Mubashar Rehman,
• Omer Aydin and
• Yin Lifang

Beilstein J. Nanotechnol. 2025, 16, 1246–1276, doi:10.3762/bjnano.16.92

• structure of protein, and reducible disulfide groups [78][79][80]. 1.3.2 Bovine serum albumin. Bovine serum albumin (BSA) is widely used in biomedical applications such as supplemental growth media and protein standards. BSA was used as a template for the synthesis of organic–inorganic hybrid nanoparticles

Serum heat inactivation diminishes ApoE-mediated uptake of D-Lin-MC3-DMA lipid nanoparticles

• Demian van Straten,
• Luuk van de Schepop,
• Rowan Frunt,
• Pieter Vader and
• Raymond M. Schiffelers

Beilstein J. Nanotechnol. 2025, 16, 740–748, doi:10.3762/bjnano.16.57

• by which FCS heat inactivation could affect cellular interactions with LNPs were studied in detail. Materials and Methods Buffers, reagents, materials Phosphate buffered saline (PBS), bovine serum albumin (BSA), thioflavin T (ThT), cholesterol and distearoylphosphatidylcholine (DSPC) were purchased

• probed with ThT, a dye that increases in fluorescence after it binds to aggregated or misfolded protein [32] (Figure 2A). Bovine serum albumin was used as a control protein as it is a major component of serum and known to be relatively heat resistant. No increase in fluorescence was seen between ApoE3

Functionalized gold nanoflowers on carbon screen-printed electrodes: an electrochemical platform for biosensing hemagglutinin protein of influenza A H1N1 virus

• Carlos Enrique Torres-Méndez,
• Sharmilee Nandi,
• Klara Martinovic,
• Patrizia Kühne,
• Yifan Liu,
• Sam Taylor,
• Maria Lysandrou,
• Maria Ines Berrojo Romeyro Mascarenhas,
• Viktoria Langwallner,
• Javier Enrique Sebastián Alonso,
• Ivana Jovanovic,
• Maike Lüftner,
• Georgia-Vasiliki Gkountana,
• David Bern,
• Abdul-Raouf Atif,
• Ehsan Manouchehri Doulabi,
• Gemma Mestres and
• Masood Kamali-Moghaddam

Beilstein J. Nanotechnol. 2025, 16, 540–550, doi:10.3762/bjnano.16.42

• , followed by blocking with bovine serum albumin to minimize nonspecific hydrophobic binding. The electrodes were characterized by cyclic voltammetry and electrochemical impedance spectroscopy experiments in the presence of [Fe(CN)6]3−/4−. Differential pulse voltammetry was used to measure the change in

• , potassium chloride, potassium hexacyanoferrate(II) trihydrate, potassium hexacyanoferrate(III), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxy succinimide (NHS), phosphate-buffered saline pH 7.4 (PBS), and bovine serum albumin (BSA) were all purchased from Sigma-Aldrich

• the section bound to the surface. This leads to proper antibody orientation such that the entire antigen binding site is available for adequate biorecognition [44]. No significant peak-to-peak separation change was observed in the CV analysis after immobilization of mAbs and blocking with bovine serum

A biomimetic approach towards a universal slippery liquid infused surface coating

• Ryan A. Faase,
• Madeleine H. Hummel,
• AnneMarie V. Hasbrook,
• Andrew P. Carpenter and
• Joe E. Baio

Beilstein J. Nanotechnol. 2024, 15, 1376–1389, doi:10.3762/bjnano.15.111

• of 1 × 108 platelets/mL [33][34]. FXIIa assay Wells of interest of a nontreated 96-well polystyrene were blocked with 300 μL 1% bovine serum albumin (BSA) in Milli-Q water for 2 h at 37 °C. After 2 h, the wells were rinsed three times with Milli-Q water. Plates were coated immediately prior to use

Interaction of graphene oxide with tannic acid: computational modeling and toxicity mitigation in C. elegans

• Romana Petry,
• James M. de Almeida,
• Francine Côa,
• Felipe Crasto de Lima,
• Diego Stéfani T. Martinez and
• Adalberto Fazzio

Beilstein J. Nanotechnol. 2024, 15, 1297–1311, doi:10.3762/bjnano.15.105

• translocation to other organs or oxidative stress. Similarly, Rive et al. [58] did not detect any detrimental effects in C. elegans exposed to amino-functionalized GO. Moreover, biomolecules interacting with the GO surface also have an effect on its toxicity, Côa et al. [14] observed that a bovine serum albumin

Dual-functionalized architecture enables stable and tumor cell-specific SiO₂NPs in complex biological fluids

• Iris Renata Sousa Ribeiro,
• Raquel Frenedoso da Silva,
• Romênia Ramos Domingues,
• Adriana Franco Paes Leme and
• Mateus Borba Cardoso

Beilstein J. Nanotechnol. 2024, 15, 1238–1252, doi:10.3762/bjnano.15.100

• adsorbed protein, and the supernatant was discarded. The bead+protein set was then incubated with 50 mM (hydroxymethyl)aminomethane buffer (Tris, pH 7.4) + 0.03% bovine serum albumin (BSA) + 0.1% Triton X for 60 min at room temperature and under manual agitation. Then, the supernatant was removed, and the

• cells were fixed with 4% paraformaldehyde for 20 min and permeabilized with 0.1% Triton X-100 and 2% bovine serum albumin for 40 min. F-actin was stained for 30 min with 10 µg/mL phalloidin-FITC (fluorescein isothiocyanate), and the nuclei were counterstained with 2 µg/mL of 4’,6-diamino-2-phenylindole

Unveiling the potential of alginate-based nanomaterials in sensing technology and smart delivery applications

• Shakhzodjon Uzokboev,
• Khojimukhammad Akhmadbekov,
• Ra’no Nuritdinova,
• Salah M. Tawfik and
• Yong-Ill Lee

Beilstein J. Nanotechnol. 2024, 15, 1077–1104, doi:10.3762/bjnano.15.88

• authors used theophylline and bovine serum albumin as drug models and alginate–starch polymer as a drug matrix for studying the controlled release. Research confirmed that modification of alginate with starch can improve the encapsulation efficiency of the matrix from 60% to 75%. Moreover, this novel drug

• nanoparticles might be further developed as a tracking device for sweat glucose detection. Another microfluidic fiber-based enzymatic biosensor was developed. Microfibers encapsulated bovine serum albumin (BSA), glucose oxidase (GOx), and horseradish peroxidase (HRP) efficiently (Figure 9). In contrast

Exfoliation of titanium nitride using a non-thermal plasma process

• Priscila Jussiane Zambiazi,
• Dolores Ribeiro Ricci Lazar,
• Larissa Otubo,
• Rodrigo Fernando Brambilla de Souza,
• Almir Oliveira Neto and
• Cecilia Chaves Guedes-Silva

Beilstein J. Nanotechnol. 2024, 15, 631–637, doi:10.3762/bjnano.15.53

• electrostatically driven exfoliation of WO3 powder using bovine serum albumin as an exfoliating agent at pH 4 [8]. Subsequently, in 2018, Balan et al. [9] achieved the synthesis of hematene, a n-vdW 2D material, from natural iron ore hematite (α-Fe2O3) using liquid exfoliation. Unlike hematite, hematene exhibited

Cholesterol nanoarchaeosomes for alendronate targeted delivery as an anti-endothelial dysfunction agent

• Horacio Emanuel Jerez,
• Yamila Roxana Simioni,
• Kajal Ghosal,
• Maria Jose Morilla and
• Eder Lilia Romero

Beilstein J. Nanotechnol. 2024, 15, 517–534, doi:10.3762/bjnano.15.46

• with PBS and fixed with 3.7% formaldehyde in PBS for 30 min. Then, cells were permeabilized with 0.1% Triton X-100 in PBS for 10 min, followed by 30 min incubation with 1% bovine serum albumin (BSA) in PBS. Finally, cells were incubated with CytoPainter Phalloidin-iFluor 488 [87] for 90 min and Hoechst

Classification and application of metal-based nanoantioxidants in medicine and healthcare

• Nguyen Nhat Nam,
• Nguyen Khoi Song Tran,
• Tan Tai Nguyen,
• Nguyen Ngoc Trai,
• Nguyen Phuong Thuy,
• Hoang Dang Khoa Do,
• Nhu Hoa Thi Tran and
• Kieu The Loan Trinh

Beilstein J. Nanotechnol. 2024, 15, 396–415, doi:10.3762/bjnano.15.36

• homeostasis in tumors through a series of cascade reactions but also establish cyclic regeneration of relevant substrates. Another study combined MnO2 nanoparticles with bovine serum albumin (BSA) to obtain GPx activity [47]. The in vitro results showed excellent biocompatibility of the MnO2-BSA nanoparticles

Study of the reusability and stability of nylon nanofibres as an antibody immobilisation surface

• Inés Peraile,
• Matilde Gil-García,
• Laura González-López,
• Nushin A. Dabbagh-Escalante,
• Juan C. Cabria-Ramos and
• Paloma Lorenzo-Lozano

Beilstein J. Nanotechnol. 2024, 15, 83–94, doi:10.3762/bjnano.15.8

• ” developed in this paper, bovine serum albumin (BSA) was chosen as a surrogate for biotoxins. In contrast, in the “Stability study” carried out in this paper, ricin was used as a representative biotoxin instead of a surrogate because the “Stability study” required less handling than the “Nanofibre

Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with Escherichia coli cells

• Eloïse Equy,
• Jordana Hirtzel,
• Sophie Hellé,
• Béatrice Heurtault,
• Eric Mathieu,
• Morgane Rabineau,
• Vincent Ball and
• Lydie Ploux

Beilstein J. Nanotechnol. 2023, 14, 1208–1224, doi:10.3762/bjnano.14.100

• applications, especially because of their biocompatibility. We synthesized and characterized fluorescent PDA NPs of 10–25 nm diameter based on a protein containing a lysine–glutamate diad (bovine serum albumin, BSA) and determined whether they can penetrate and accumulate in bacterial cells to serve as a

• protein and to determine whether they can enter and accumulate in bacterial cells. The investigation has been conducted with NPs made of polydopamine (PDA) and bovine serum albumin (BSA), and Escherichia coli (E. coli) bacteria as a bacterial model. Three different types of fluorescent BSA/PDA NPs have

• . Experimental Synthesis Reagents Dopamine (DA) hydrochloride and bovine serum albumin (BSA) were purchased from Merck (Darmstadt, Germany) and used without purification. Fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RhBITC), hydrochloric acid 37% (wt/v), absolute ethanol and Hellmanex® were

Recognition mechanisms of hemoglobin particles by monocytes – CD163 may just be one

• Jonathan-Gabriel Nimz,
• Pichayut Rerkshanandana,
• Chiraphat Kloypan,
• Ulrich Kalus,
• Saranya Chaiwaree,
• Axel Pruß,
• Radostina Georgieva,
• Yu Xiong and
• Hans Bäumler

Beilstein J. Nanotechnol. 2023, 14, 1028–1040, doi:10.3762/bjnano.14.85

• might not be their Hb content. In their work from 2013, Yan et al. [40] showed how the formation of a protein corona influences particle–cell interactions. Especially bovine serum albumin (BSA) showed an ambivalent effect. On the one hand, the corona, which consisted mainly of BSA, reduced the direct

Carboxylic acids and light interact to affect nanoceria stability and dissolution in acidic aqueous environments

• Matthew L. Hancock,
• Eric A. Grulke and
• Robert A. Yokel

Beilstein J. Nanotechnol. 2023, 14, 762–780, doi:10.3762/bjnano.14.63

• , distribution, and toxicity of nanoceria within biological systems. Cellular uptake studies of nanoceria in lung adenocarcinoma (A549) cells favored particles with a negative zeta potential. However, positively charged particles resulted in greater bovine serum albumin adsorption. This suggests that surface

Gap-directed chemical lift-off lithographic nanoarchitectonics for arbitrary sub-micrometer patterning

• Chang-Ming Wang,
• Hong-Sheng Chan,
• Chia-Li Liao,
• Che-Wei Chang and
• Wei-Ssu Liao

Beilstein J. Nanotechnol. 2023, 14, 34–44, doi:10.3762/bjnano.14.4

• Grove, PA, USA). Bovine serum albumin (BSA) and 10× phosphate buffered saline (PBS, contains 1.37 M NaCl, 0.027 M KCl, 0.10 M Na2HPO4, and 0.018 M KH2PO4) were purchased from Bioman Scientific Co., Ltd. (Taipei, Taiwan). Ethanol (99.5 wt %), acetone (99.5 wt %), and isopropanol (99.9 wt %) were

Design of surface nanostructures for chirality sensing based on quartz crystal microbalance

• Yinglin Ma,
• Xiangyun Xiao and
• Qingmin Ji

Beilstein J. Nanotechnol. 2022, 13, 1201–1219, doi:10.3762/bjnano.13.100

• modified QCM sensors [29]. The sensor layers were constructed by self-assembly of ʟ(ᴅ)-Val monolayers or comb-teeth-type grafted polymer brushes with ʟ(ᴅ)-Val. According to the frequency shifts upon bovine serum albumin (BSA) adsorption, ʟ-Val-modified sensors were shown to have a stronger binding to BSA

Stimuli-responsive polypeptide nanogels for trypsin inhibition

• Petr Šálek,
• Jana Dvořáková,
• Sviatoslav Hladysh,
• Diana Oleshchuk,
• Ewa Pavlova,
• Jan Kučka and
• Vladimír Proks

Beilstein J. Nanotechnol. 2022, 13, 538–548, doi:10.3762/bjnano.13.45

• confirmed by dynamic light scattering and zeta potential measurements. The loading capacity of PHEG-Tyr and Nα-Lys-NG nanogels and their release profiles were first optimized with bovine serum albumin. The nanogels were then used for loading and release of AAT. PHEG-Tyr and Nα-Lys-NG nanogels showed

• acid-labile cholesteryl-modified pullulan nanogel that formed a complex with loaded bovine serum albumin and released it under acidic conditions [17]. Landfester et al. used a bio-orthogonal reaction to fabricate a dextran nanogel with pH-responsive hydrazine linkages allowing for a controlled release

• optimizing the loading and release from PHEG-Tyr and Nα-Lys-NG with bovine serum albumin (BSA), loading and release of AAT were investigated. The results show that AAT has a higher affinity to Nα-Lys-NG and both nanogel depots demonstrate burst release over 6 h followed by continuous release over ca. 160 h

Zinc oxide nanostructures for fluorescence and Raman signal enhancement: a review

• Ioana Marica,
• Fran Nekvapil,
• Maria Ștefan,
• Cosmin Farcău and
• Alexandra Falamaș

Beilstein J. Nanotechnol. 2022, 13, 472–490, doi:10.3762/bjnano.13.40

• were successfully used for the detection of relevant biological and biomedical proteins, such as bovine serum albumin and streptavidin, as well as to study the protein–protein interactions by enhanced fluorescence detection [117]. The ZnO nanoplatforms showed several key advantages, such as enhanced

Effects of drug concentration and PLGA addition on the properties of electrospun ampicillin trihydrate-loaded PLA nanofibers

• Tuğba Eren Böncü and
• Nurten Ozdemir

Beilstein J. Nanotechnol. 2022, 13, 245–254, doi:10.3762/bjnano.13.19

• properties values (p < 0.05). This was similar to our previous studies with linezolid-loaded PLGA and PCL/PLGA nanofibers [16][30]. Chew et al. (2006) also showed that an increase in bovine serum albumin caused an increase in nanofiber diameter and a decrease in mechanical properties of poly(caprolactone-co

A comprehensive review on electrospun nanohybrid membranes for wastewater treatment

• Senuri Kumarage,
• Imalka Munaweera and
• Nilwala Kottegoda

Beilstein J. Nanotechnol. 2022, 13, 137–159, doi:10.3762/bjnano.13.10

• work, a mildly hydrolyzed PAN membrane has been modified by fusing bovine serum albumin (BSA) obtained from laboratory wastes, and it has shown a maximum dye removal capacity of 434.78 mg/g, at pH 12 for TBO. The membrane has the ability to be regenerated by eluting the dye completely with 1 M NaCl or

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

• Rouhollah Khodadust,
• Ozlem Unal and
• Havva Yagci Acar

Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6

• was recorded using a UV–vis spectrophotometer (Shimadzu UV-3600 UV–vis–NIR spectrophotometer). A calibration curve was prepared with bovine serum albumin (BSA) at concentration values of 0, 1, 2, 4, 6, 8, and 10 µg/mL in PBS to enable a correlation between the measured absorbance and protein

An overview of microneedle applications, materials, and fabrication methods

• Zahra Faraji Rad,
• Philip D. Prewett and
• Graham J. Davies

Beilstein J. Nanotechnol. 2021, 12, 1034–1046, doi:10.3762/bjnano.12.77

• incorporated drug. A wide range of molecules including the anthrax vaccine [26], aminolevulinic acid [27], calcein [28], erythropoietin [29], bovine serum albumin [30][31], ovalbumin [32], insulin [33][34], and plasmid DNA [35] have been transdermally delivered using microneedles of various designs, aimed at a

• microneedles of soluble poly(lactic-co-glycolic acid) (PLGA) and PLGA–polyvinylpyrrolidone (PLGA–PVP) layered combinations have been used to provide controlled drug delivery of bovine serum albumin (BSA), rather than instantaneous release [60]. There are only a few published studies demonstrating the