Beilstein J. Nanotechnol.2014,5, 872–880, doi:10.3762/bjnano.5.99
-CS-Fe3O4@EUG nanospheres diameter sizes range between 20 and 80 nm. These MAPLE-deposited coatings acted as bioactive nanosystems and exhibited a great antimicrobial effect by impairing the adherence and biofilm formation of Staphylococcus aureus (S. aureus) and Pseudomonasaeruginosa (P. aeruginosa
driven by the Axio-Vision 4.6 (Carl Zeiss, Germany) software.
In vitro microbial biofilm development
Staphylococcus aureus ATCC 25923 and Pseudomonasaeruginosa ATCC 27853 strains were purchased from American Type Cell Collection (ATCC, USA). For the biofilm assays, fresh bacteria cultures were obtained
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Figure 1:
TEM images of prepared Fe3O4@EUG nanoparticles.
Beilstein J. Nanotechnol.2013,4, 345–351, doi:10.3762/bjnano.4.40
investigated against Gram-positive Staphylococcus aureus (S. aureus), and Gram-negative Pseudomonasaeruginosa (P. aeruginosa) and Escherichia coli (E. coli) bacteria under visible light.
Results and Discussion
XRD of TiO2 and Ag-doped TiO2
The samples were annealed at 450 °C to achieve crystallization in TiO2
and transfer behaviour of the photoexcited electron–hole pairs in the semiconductors was recorded by photoluminescence. The antimicrobial activity of TiO2 and Ag-doped TiO2 nanoparticles (3% and 7%) was investigated against both gram positive (Staphylococcus aureus) and gram negative (Pseudomonas
aeruginosa, Escherichia coli) bacteria. As a result, the viability of all three microorganisms was reduced to zero at 60 mg/30 mL culture in the case of both (3% and 7% doping) concentrations of Ag-doped TiO2 nanoparticles. Annealed TiO2 showed zero viability at 80 mg/30 mL whereas doped Ag-TiO2 7% showed
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Figure 1:
XRD pattern of (a) TiO2 and (b) 3% and (c) 7% Ag-doped TiO2 nanoparticles annealed at 450 °C.