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Search for "proliferation" in Full Text gives 171 result(s) in Beilstein Journal of Nanotechnology.
Silicon microgrooves for contact guidance of human aortic endothelial cells
Beilstein J. Nanotechnol. 2017, 8, 675–681, doi:10.3762/bjnano.8.72
- cell adhesion, morphology and proliferation were assessed, by comparing them to flat silicon substrates, used as control condition. Using human aortic endothelial cells, microscopy images demonstrate that the cellular response is different depending on the silicon surface, when it comes to cell
- adhesion, morphology (alignment, circularity and filopodia presence) and proliferation. Moreover, these structures exerted no cytotoxic effect. Conclusion: The results suggest that topographical patterning influences cell response. Silicon groove substrates can be used in developing medical devices with
- chemistry influence cellular behaviour such as adhesion, migration and proliferation [1][2][3][4][5][6]. It is important to understand and control cell behaviour by topography in order to modulate the functions of the cells. Cells react to topographic stimuli through a process known as mechanotransduction
Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles
Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40
- this protein family in humans are HTRA1 and HTRA2 that are both involved in tumour suppression and in the control of proliferation, migration, and neurodegeneration [31]. The HTRA1 gene (previously termed PRSS11) was initially identified in human fibroblasts [32]. Numerous experimental findings suggest
- a tumour suppressor function for HTRA1. In various cancer types, an epigenetic silencing of HTRA1 has been observed. Moreover, a decreased HTRA1 expression is correlated both with a reduced response to chemotherapeutics and an augmented cell migration. Additionally, a reduced proliferation of tumour
Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina
Beilstein J. Nanotechnol. 2017, 8, 74–81, doi:10.3762/bjnano.8.8
- surface roughness [3][4], the latter of which can play an important role in the adhesion and proliferation of cells [5][6][7]. The self-ordered nano-structured APA, also demonstrated recently as a possible nanolithographic mask [8][9] and for chemical sensors and biosensors [10], after coating with noble
Facile fabrication of luminescent organic dots by thermolysis of citric acid in urea melt, and their use for cell staining and polyelectrolyte microcapsule labelling
Beilstein J. Nanotechnol. 2016, 7, 1905–1917, doi:10.3762/bjnano.7.182
- also known that the MTT assay is typically used for quantifying metabolically active cells, independently of proliferation [63]. Therefore, the highest concentration of sample that caused, in the treated cells, the activation of NADP-H-dependent oxidoreductases, not more than in the intact cells, was
Low temperature co-fired ceramic packaging of CMOS capacitive sensor chip towards cell viability monitoring
Beilstein J. Nanotechnol. 2016, 7, 1871–1877, doi:10.3762/bjnano.7.179
- cell proliferation thus seemed to be normal, the use of the LTCC package for the sensor chip was regarded as promising [23]. Here we have tested version 2 of the LTCC package made from DupontTM 951 LTCC tape instead of the Heraeus HeraLock® Tape HL2000 (no longer produced) used in our earlier version
Nano- and microstructured materials for in vitro studies of the physiology of vascular cells
Beilstein J. Nanotechnol. 2016, 7, 1620–1641, doi:10.3762/bjnano.7.155
- and their biological responses to micro- and nanostructured surfaces are reviewed. Emphasis is given to studies of cell morphology and motility, cell proliferation, the cytoskeleton and cell-matrix adhesions, and signal transduction pathways of vascular cells. We finalize with a short outlook on
- following section, a brief summary of the most common methods for control of the surface biochemistry and of the mechanical properties of the substrate are given. 1.5 Surface (bio)functionalization The surface (bio)chemistry of a material may regulate cell adhesion, survival, proliferation and
- ][173][174][175][176]. Thereby, a multitude of stimulating signals, such as messenger molecules, ECM, pulsatile blood flow and endogenous electrical fields exist in and around the vasculature [177][178][179]. Additionally, in blood vessels of healthy humans, the regulation of SMC proliferation and
Influence of hydrothermal synthesis parameters on the properties of hydroxyapatite nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 1586–1601, doi:10.3762/bjnano.7.153
- proliferation of such malignant cells was inhibited more efficiently by the occurrence of the nanoscale effect than by HAp particle morphology [9]. Another study analyzed the effects of different sized nano-HAp – ranging from 20 to 80 nm – on the proliferation of bone-related cells (bone marrow mesenchyme stem
Viability and proliferation of endothelial cells upon exposure to GaN nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 1330–1337, doi:10.3762/bjnano.7.124
- ) semiconductor nanoparticles. Cellular viability, adhesion, proliferation, and uptake of nanoparticles by endothelial cells were investigated. The effect of free GaN nanoparticles versus the effect of growing endothelial cells on GaN functionalized surfaces was examined. To functionalize surfaces with GaN, GaN
- cells grown on surfaces functionalized with GaN nanoparticles demonstrated excellent adhesion and proliferation, suggesting good biocompatibility of the nanostructured GaN. Keywords: endothelial cells; GaN nanoparticles; proliferation; surface functionalization; Introduction The development of new
- the mechanical load of incorporated nanoparticles, which could result in reduced migration and slowed proliferation process with increasing quantities of incorporated nanoparticles [20]. When nanoparticles were added after the cellular layer reached 50% confluence, we clearly observed how the same
On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 1296–1311, doi:10.3762/bjnano.7.121
- . Similar results were reported by Zhang et al. comparing 80 nm SiNPs with 500 nm SiNPs on HepG2 cells [40]. Those investigated particles affect cell viability and the proliferation potential in a size-ascending-dependent manner. Nevertheless, these data were only focusing on large differences in size and
Reasons and remedies for the agglomeration of multilayered graphene and carbon nanotubes in polymers
Beilstein J. Nanotechnol. 2016, 7, 1174–1196, doi:10.3762/bjnano.7.109
- conductive polymer nanocomposites can be produced. However, all these enhancements of the performance of polymers can only be achieved when the filler is uniformly dispersed and no agglomeration of filler in the polymer matrix takes place. MLG and CNTs have been reported to promote the proliferation of
Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84
- their cellular uptake, the mechanism of internalization, cytotoxicity, viability and proliferation of neural stem cells, and compared them to the commercially available dextran-coated nanomag®-D-spio nanoparticles. Results: Light microscopy of Prussian blue staining revealed a concentration-dependent
- intracellular uptake of iron oxide in neural stem cells. The methyl thiazolyl tetrazolium assay and the calcein acetoxymethyl ester/propidium iodide assay demonstrated that poly(L-lysine)-coated maghemite nanoparticles scored better than nanomag®-D-spio in cell labeling efficiency, viability and proliferation
- follow the stem cells through their migration, distribution, proliferation and differentiation is an essential prerequisite to characterize the biology and behavior of stem cells, to design the therapeutic approaches and minimize possible side effects [8][9][10]. Magnetic nanoparticles are widely used to
Tight junction between endothelial cells: the interaction between nanoparticles and blood vessels
Beilstein J. Nanotechnol. 2016, 7, 675–684, doi:10.3762/bjnano.7.60
- inhibited (79%) by silver nanoparticles (2–6 nm) [80]. Also, AuNPs have the ability to prevent vascular endothelial growth factor (VEGF)- and interleukin-1 beta (IL-1β)-induced proliferation and migration in bovine retinal pigment epithelial cells (BRPEs) through the suppression of the Src kinase pathway
Unraveling the neurotoxicity of titanium dioxide nanoparticles: focusing on molecular mechanisms
Beilstein J. Nanotechnol. 2016, 7, 645–654, doi:10.3762/bjnano.7.57
- expression. These stable alternations are not caused by changes to DNA sequence itself, but instead arise during development and cell proliferation [13][14]. DNA methylation is the one of the most extensively studied epigenetic mechanisms. Whether TiO2 NPs are able to induce neurotoxicity through altering
- nasal administration for nine months, after which the glial cells showed over-proliferation and tissue necrosis was found in hippocampal area. Meanwhile, the expression of genes associated with neurotrophin signaling pathways, such as nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF
- tumor necrosis factor-α (TNF-α) in the mouse hippocampus was promoted. At the same time, histopathological changes were observed in the hippocampus; over-proliferation of glial cells, impaired nuclei, and cellular degeneration were observed, all of which contributed to neuro-inflammation. In addition
Antibacterial activity of silver nanoparticles obtained by pulsed laser ablation in pure water and in chloride solution
Beilstein J. Nanotechnol. 2016, 7, 465–473, doi:10.3762/bjnano.7.40
- about 2 h and increases at 3 h, with no detectable CFUs after 24 h of incubation (Table 3). Hence, according to our data on ns-ablated AgNPs, the minimum time necessary to achieve bactericidal effect was longer than 3 h. Nevertheless, such AgNPs are very quick at inhibiting bacterial proliferation, when
Fabrication of hybrid nanocomposite scaffolds by incorporating ligand-free hydroxyapatite nanoparticles into biodegradable polymer scaffolds and release studies
Beilstein J. Nanotechnol. 2015, 6, 2217–2223, doi:10.3762/bjnano.6.227
- similarity to the mineral constituent of human bones, are bioactive and can be fairly easily bioconjugated [1]. HA NPs can enhance cell proliferation in bone tissue regeneration [2]. Tissue engineering is an interdisciplinary field that combines the principles of life sciences and engineering to improve
- of HA enhanced hydrophilicity and serum protein adsorption, and as a result, this increased pre-osteoblast cell attachment, spreading, and proliferation after four days of culture. Different technical routes have been explored for the synthesis of HA NPs, including mechanochemical synthesis [10
PLGA nanoparticles as a platform for vitamin D-based cancer therapy
Beilstein J. Nanotechnol. 2015, 6, 1306–1318, doi:10.3762/bjnano.6.135
- in the S or/and G2/M phases (Figure 6B,C). Additionally, the observed changes on the cell cycle distribution between control A549 cells and A549 cells treated with free calcitriol for 72 h were not significant (p > 0.05). These results are in agreement with the proliferation studies, where the A549
- pancreatic cell lines, S2-013 and hTERT-HPNE, was reported. The in vitro proliferation assay showed that the encapsulation of calcitriol enhanced its antiproliferative activity. The efficient cell internalization by an endocytosis mechanism of PLGA NPs and their rapid endo-lysosomal escape observed in this
Influence of gold, silver and gold–silver alloy nanoparticles on germ cell function and embryo development
Beilstein J. Nanotechnol. 2015, 6, 651–664, doi:10.3762/bjnano.6.66
- effect was caused by silver ions. However, it supports findings made on spermatogonial stem cells in vitro, which claimed a decrease in cell proliferation after AgNP exposure [40][41]. Observations concerning female reproductive organs are rather rare as most nanoparticle biodistribution studies have
Self-assembled anchor layers/polysaccharide coatings on titanium surfaces: a study of functionalization and stability
Beilstein J. Nanotechnol. 2015, 6, 617–631, doi:10.3762/bjnano.6.63
- molecules) formed by ionic cross-linking [40]. The proposed architecture is envisaged to enhance adhesion, proliferation, differentiation of osteoblasts, and thus ultimately, to achieve a better integration of the titanium implant into the bone tissue. Conclusion In the present contribution, we demonstrated
Novel ZnO:Ag nanocomposites induce significant oxidative stress in human fibroblast malignant melanoma (Ht144) cells
Beilstein J. Nanotechnol. 2015, 6, 570–582, doi:10.3762/bjnano.6.59
- ) assay the cytotoxicity in vitro and the differential effect of different Ag contents in the ZnO nanoparticles affecting cell proliferation was analyzed. The photo-oxidation-mediated cytotoxicity of different NPs was investigated by irradiating the samples with daylight or keeping them in the dark. The
- (565)NP control and Abs(565)blank represent the background optical density and was measured in NPs only and media only samples. HCEC cells were included as a normal control cell line. Mitochondrial function: cell survival and proliferation assay MTT assay was used to investigate mitochondrial function
Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy
Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57
- biocompatibility of these NPs was investigated by standard MTT cell proliferation assay. Studies suggested that the cell viability was maintained at 83% even after a high dose of 500 µg·mL−1 of the nanocomposites. To check the applicability of these nanocomposites as fluorescence imaging agents, Gastric SGC7901
Pulmonary surfactant augments cytotoxicity of silica nanoparticles: Studies on an in vitro air–blood barrier model
Beilstein J. Nanotechnol. 2015, 6, 517–528, doi:10.3762/bjnano.6.54
- ® in the well was 0.04 mg/mL. Cytotoxicity, determination of cell viability: The viability of the cells was determined as described in our previous studies [9][10][11] using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS, Promega, G3582). After nanoparticle incubation, medium was
Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation
Beilstein J. Nanotechnol. 2015, 6, 383–395, doi:10.3762/bjnano.6.38
- nanoparticles. This was very much expected as the proliferation of hHSCs during the growth factor induced differentiation will decrease the amount of nanoparticles per cell division by a factor of 1/2. For the PLLA particles, the remaining amount was significantly higher than the negative control even after 11
- . The hHSCs showed a great loss in particle payload during all three lineage differentiations. This is likely due to the high proliferation rates during the differentiation process. In other studies, this dilution due to proliferation activity of the cells has clearly been shown [28][29]. In the
- the high expression of glycophorin A, which indicates that the erythrocyte differentiation is enhanced with PLLA–Fe particles. This can be explained by the fact that iron in this case is an essential compound for erythrocyte differentiation and, therefore, enhances proliferation in this direction
Comparative evaluation of the impact on endothelial cells induced by different nanoparticle structures and functionalization
Beilstein J. Nanotechnol. 2015, 6, 300–312, doi:10.3762/bjnano.6.28
- used a colorimetric (MTS: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; Aqueous One Solution Cell Proliferation Assay, Promega, Germany) and a luminescence (ATPLite assay, PerkinElmer, Germany) based cytotoxicity assay. In this context, cells were
The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots
Beilstein J. Nanotechnol. 2015, 6, 281–292, doi:10.3762/bjnano.6.26
- proliferation, which was confirmed by fluorescence microscopy (Supporting Information File 1, Figure S1). We observed a small fraction of abnormally large cells (twice as large as the normal size), and cells with two nuclei after exposure to the 50 nM solutions of QDs (Supporting Information File 1, Figure S1
Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants
Beilstein J. Nanotechnol. 2015, 6, 254–262, doi:10.3762/bjnano.6.24
- surface hydrophilicity by forming oxygen-containing groups at the surface and thus to improve cell adhesion and proliferation. The conditions of the plasma modification were properly adjusted in order to induce the desirable chemical surface changes while maintaining surface integrity and morphology. The
- , which is more apparent in the case of the treated samples indicates the growth and proliferation of the cells in their new microenvironment. Cell adhesion and proliferation According to Figure 4c, fibroblasts seemed to be securely attached and spread on the surface, regardless of the surface treatments
- . Interestingly, cells seemed to be attached and spread more on the plasma-treated scaffold surfaces compared to the untreated surfaces, during the first day. A notable enhancement of the spreading is observed in the following days, with higher cell confluency and proliferation in the treated scaffolds compared
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