Beilstein J. Nanotechnol.2020,11, 354–369, doi:10.3762/bjnano.11.26
procured from HiMedia, USA. Ribogreen reagent was purchased from Invitrogen, USA. All other reagents of analytical grade were purchased from Merck, India. 5-Fluorouracil (5-FU) was procured from Sigma-Aldrich, USA. VEGF antibody (Santa Cruz Biotechnology Ltd., USA), β- actin and other antibodies (Cell
reader (Epoch i2, Biotek, USA). For assessing the effect of VEGF silencing on the cytotoxicity of 5-FU, the cells were initially treated with the polyplex or with free siRNA at a siRNA concentration of 100 nM for 4 h. The medium was then replaced with fresh medium to which 400 μM of 5-FU was added and
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Cell viability measurements
VEGF inhibition can also be used in combination with chemotherapeutic agents to enhance their therapeutic efficacy. The ability of the polyplex to alter the cell viability of A549 cells treated with 5-FU was investigated and the results are represented in Figure 12. The
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Figure 1:
(A) Gel retardation assay for polyplexes formed with different ratios (v/v) of PVI/siRNA. Ethidium ...
Beilstein J. Nanotechnol.2018,9, 2499–2515, doi:10.3762/bjnano.9.233
excellent loading efficiency, biodegradable nature and controlled-release behaviour. Herein, we report a novel system of 5-fluorouracil (5-FU)-loaded calcium phosphate nanoparticles (CaP@5-FU NPs) synthesized via a reverse micelle method. The formation of monodispersed, spherical, crystalline nanoparticles
with an approximate diameter of 160–180 nm was confirmed by different methods. The physicochemical characterization of the synthesized CaP@5-FU NPs was done with transmission electron microscopy (TEM), dynamic light scattering (DLS), field emission scanning electron microscopy (FE-SEM), Fourier
-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The antineoplastic potential of the CaP@5-FU NPs against colorectal and lung cancer cells was reported. The CaP@5-FU NPs were found to inhibit half the population (IC50) of lung adenocarcinoma (A549) cells at 32 μg/mL and colorectal (HCT
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Figure 1:
(A) Schematic representation for the preparation of CaP@5-FU NPs. (B) The UV–visible spectrum of Ca...