New application of bimetallic Ag/Pt nanoplates in a colorimetric biosensor for specific detection of E. coli in water

• Azam Bagheri Pebdeni,
• Mohammad N. AL-Baiati and
• Morteza Hosseini

Beilstein J. Nanotechnol. 2024, 15, 95–103, doi:10.3762/bjnano.15.9

• sodium hydroxide were purchased from Sigma. Tris-EDTA and HCl (for TE buffer production) were purchased from Merck. The aptamer for identifying E. coli (5′-CCC CCC CCC CCG GAC GCT TAT GCC TTG CCA TCT ACA GAG CAG GTG TGA CGG-3′) was acquired from Pishgam (Tehran, Iran). Lyophilized bacterial strains and

Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

• Nittiya Suwannasom,
• Netsai Sriaksorn,
• Chutamas Thepmalee,
• Krissana Khoothiam,
• Ausanai Prapan,
• Hans Bäumler and
• Chonthida Thephinlap

Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93

• Na2CO3 solutions in the presence of albumin [26][27]. This approach has been shown to have high effectiveness for entrapping various biopolymers (e.g., proteins and hemoglobin) into carbonate particles before dissolving with EDTA to obtain pure biopolymer submicron particles. The CCD technique is a

• -HSA-MPs and HSA was cross-linked. After dissolution of the MnCO3 template with EDTA, a colloidal particle suspension of CUR-HSA-MPs was obtained. Comparison of the different CUR samples demonstrated that the color of CUR in DMSO is light brown, indicating dissolved CUR; CUR in water is bright yellow

• albumin proteins [30]. CUR showed strong binding affinity towards HSA, likely at the tryptophan residue, which is present in the hydrophobic cavities of HSA [23][31]. The final CUR-HSA-MPs are obtained after dissolution of the MnCO3 template with EDTA. Characterization of CUR-HSA-MPs Dynamic light

Recognition mechanisms of hemoglobin particles by monocytes – CD163 may just be one

• Jonathan-Gabriel Nimz,
• Pichayut Rerkshanandana,
• Chiraphat Kloypan,
• Ulrich Kalus,
• Saranya Chaiwaree,
• Axel Pruß,
• Radostina Georgieva,
• Yu Xiong and
• Hans Bäumler

Beilstein J. Nanotechnol. 2023, 14, 1028–1040, doi:10.3762/bjnano.14.85

• % solution was purchased from PAN-Biotech GmbH, Aidenbach, Germany. Manganese chloride, sodium hydroxide, sodium borohydride, sodium carbonate, ethylenediaminetetraacetic acid (EDTA), glutaraldehyde solution, grade II, 25% in H2O, as well as Triton™ X-100 were purchased from Sigma-Aldrich, Steinheim, Germany

• h at room temperature under stirring. After another washing in aqua dest. the MnCO3 matrix was dissolved using 0.25 M EDTA. After 30 min incubation time, 0.2 mg/mL NaBH4 in 0.1 M NaOH was added to prevent oxidation of Hb to met-Hb. Incubation for another 30 min followed. After triplicate washing in

Nanostructured lipid carriers containing benznidazole: physicochemical, biopharmaceutical and cellular in vitro studies

• Giuliana Muraca,
• María Esperanza Ruiz,
• Rocío C. Gambaro,
• Sebastián Scioli-Montoto,
• María Laura Sbaraglini,
• Gisel Padula,
• José Sebastián Cisneros,
• Cecilia Yamil Chain,
• Vera A. Álvarez,
• Cristián Huck-Iriart,
• Guillermo R. Castro,
• María Belén Piñero,
• Matias Ildebrando Marchetto,
• Catalina Alba Soto,
• Germán A. Islan and
• Alan Talevi

Beilstein J. Nanotechnol. 2023, 14, 804–818, doi:10.3762/bjnano.14.66

• 50 µM) or the free drug were added. After 72 h of treatment, the cells were harvested with a trypsin/EDTA solution and processed for flow cytometry analysis using a BD Biosciences FACSCANTO II Flow Cytometer (Franklin Lakes, NJ, USA). Propidium iodide (Sigma, St. Louis, USA) was added to the cell

In search of cytotoxic selectivity on cancer cells with biogenically synthesized Ag/AgCl nanoparticles

• Mitzi J. Ramírez-Hernández,
• Mario Valera-Zaragoza,
• Omar Viñas-Bravo,
• Ariana A. Huerta-Heredia,
• Miguel A. Peña-Rico,
• Erick A. Juarez-Arellano,
• David Paniagua-Vega,
• Eduardo Ramírez-Vargas and
• Saúl Sánchez-Valdes

Beilstein J. Nanotechnol. 2022, 13, 1505–1519, doi:10.3762/bjnano.13.124

• solution made of trypsin-EDTA was added and incubated at 37 °C for 3 min in a humidified incubator with 5% CO2 to produce a cell suspension. Trypsinized cells were reseeded in fresh medium at 105 cells/mL and incubated at 37 °C in a 5% CO2 humidified incubator. All reagents were purchased from Biowest

Hydroxyapatite–bioglass nanocomposites: Structural, mechanical, and biological aspects

• Olga Shikimaka,
• Mihaela Bivol,
• Bogdan A. Sava,
• Marius Dumitru,
• Christu Tardei,
• Beatrice G. Sbarcea,
• Daria Grabco,
• Constantin Pyrtsac,
• Daria Topal,
• Andrian Prisacaru,
• Vitalie Cobzac and
• Viorel Nacu

Beilstein J. Nanotechnol. 2022, 13, 1490–1504, doi:10.3762/bjnano.13.123

• quantity of 0.25% trypsin/0.02% EDTA. The cells were transferred in a 75 cm2 flask and cultured in mesenchymal stem cell expansion medium under the same conditions, changing the cell culture medium every second day, until 70–90% cellular confluence. After detachment, the cells were split and cultured in

Recent advances in green carbon dots (2015–2022): synthesis, metal ion sensing, and biological applications

• Aisha Kanwal,
• Naheed Bibi,
• Sajjad Hyder,
• Arif Muhammad,
• Hao Ren,
• Jiangtao Liu and
• Zhongli Lei

Beilstein J. Nanotechnol. 2022, 13, 1068–1107, doi:10.3762/bjnano.13.93

• by the use of vacuum filtration to separate the hydrothermal product. The hydrochar was refluxed in nitric and sulfuric acid, and dialysis membranes of different sizes were used to get CDs emitting four different colors, that is, blue, green, yellow, and red (Figure 7A,B) [118]. EDTA was used in a

Spindle-like MIL101(Fe) decorated with Bi₂O₃ nanoparticles for enhanced degradation of chlortetracycline under visible-light irradiation

• Chen-chen Hao,
• Fang-yan Chen,
• Kun Bian,
• Yu-bin Tang and
• Wei-long Shi

Beilstein J. Nanotechnol. 2022, 13, 1038–1050, doi:10.3762/bjnano.13.91

• investigated by radical trapping test. Ethylenediaminetetraacetic acid disodium (EDTA-2Na), isopropanol (IPA), and vitamin C (VC) were used as scavengers for holes (h+), hydroxyl radicals (•OH), and superoxide radicals (•O2−), respectively. Figure 8a shows the degradation rate of CTC in the presence of three

• scavengers. As shown in Figure 8a, when VC was added to the photocatalytic reaction system, the degradation efficiency of CTC significantly decreased from 88.2% to 12.5%, which revealed that •O2− is main active species in the degradation of CTC. In addition, after the addition of IPA and EDTA-2Na, the

DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection

• Nathalie B. F. Almeida,
• Thiago A. S. L. Sousa,
• Viviane C. F. Santos,
• Camila M. S. Lacerda,
• Thais G. Silva,
• Rafaella F. Q. Grenfell,
• Flavio Plentz and
• Antero S. R. Andrade

Beilstein J. Nanotechnol. 2022, 13, 873–881, doi:10.3762/bjnano.13.78

• -fidelity PCR buffer. PCR thermocycling was performed as described above. Conventional and asymmetric PCR reactions were carried out in a Veriti thermal cycler (Applied Biosystems, Foster City, CA). PCR amplification was verified by electrophoresis on 2% agarose gels (in tris–borate–EDTA buffer) stained

Hierarchical Bi₂WO₆/TiO₂-nanotube composites derived from natural cellulose for visible-light photocatalytic treatment of pollutants

• Zehao Lin,
• Zhan Yang and
• Jianguo Huang

Beilstein J. Nanotechnol. 2022, 13, 745–762, doi:10.3762/bjnano.13.66

• , anhydrous ethanol, acetone, barium sulfate (BaSO4), rhodamine B, isopropyl alcohol (IPA), N-methylpyrrolidone, ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), silver nitrate (AgNO3), sodium sulfate (Na2SO4), ethylene glycol (EG), potassium dichromate (K2Cr2O7), phosphoric acid (H3PO4), and

• Cr(VI), IPA (0.1 M), EDTA-2Na (10.0 mM), KIO3 (0.1 M), and AgNO3 (0.1 M) were added into the reaction solution to shield the hydroxyl radicals (•OH), reactive holes (h+), superoxide radicals (•O2−) and electrons (e−) species, respectively, generated during the photocatalysis. Similarly, IPA (0.1 M

• ), EDTA-2Na (0.1 M), and p-BQ (5.0 mM) were also put into the initial RhB pollutant solution to capture •OH, h+, and •O2− species, respectively, generated during the photocatalytic degradation of RhB. Results and Discussion Structural characterization As shown in Figure 1, the Bi2WO6/TiO2-NT

Sodium doping in brookite TiO₂ enhances its photocatalytic activity

• Boxiang Zhuang,
• Honglong Shi,
• Honglei Zhang and
• Zeqian Zhang

Beilstein J. Nanotechnol. 2022, 13, 599–609, doi:10.3762/bjnano.13.52

• performance of brookite mainly depends on the details of the preparation method and heat treatment used. For example, brookite TiO2 prepared from a Ti–EDTA complex exhibits a considerably higher activity for the degradation of NO than that synthesized from the titanate–glycolate complex [19]. The

Ethosomal (−)-epigallocatechin-3-gallate as a novel approach to enhance antioxidant, anti-collagenase and anti-elastase effects

• Çiğdem Yücel,
• Gökçe Şeker Karatoprak,
• Sena Yalçıntaş and
• Tuğba Eren Böncü

Beilstein J. Nanotechnol. 2022, 13, 491–502, doi:10.3762/bjnano.13.41

• (−)-Epigallocatechin-3-gallate, 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), SPC, trypsin-EDTA solution, dimethyl sulfoxide (DMSO) for cell culture, and penicillin/streptomycin solution were purchased from Sigma, USA. Carbopol 980 was purchased from the Abdi İbrahim Pharmaceutical, Industry and

Bacterial safety study of the production process of hemoglobin-based oxygen carriers

• Axel Steffen,
• Yu Xiong,
• Radostina Georgieva,
• Ulrich Kalus and
• Hans Bäumler

Beilstein J. Nanotechnol. 2022, 13, 114–126, doi:10.3762/bjnano.13.8

• final particle suspensions. Therefore, we conclude that the HbMP fabrication procedure is safe in respect of bacterial contamination. Keywords: bacterial safety; EDTA; glutaraldehyde; hemoglobin; microparticles; Introduction Artificial oxygen carriers used as red blood cell (RBC) substitutes have

• of the salt template by adding ethylenediaminetetraacetic acid (EDTA) results in submicrometer hemoglobin particles. The resulting particles have an advantageous oxygen affinity and show a narrow size distribution of approx. 700 nm. In the first pre-clinical investigations it was shown that HbMP meet

• known to have an antibacterial effect and it is used as a disinfectant or for cold sterilization of medical instruments in hospitals at higher concentrations [22][23][24]. Glutaraldehyde is also widely used in biochemical applications and as a fixative for electron microscopy [25][26]. EDTA is used in

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

• Rouhollah Khodadust,
• Ozlem Unal and
• Havva Yagci Acar

Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6

• -EDTA (TBE) (Alfa Aesar, USA) buffer and boiled at 100 °C. After cooled down to 70 °C, 4 µL of ethidium bromide (10 mg/mL) was added and the gel was poured into a gel electrophoresis unit. A volume of 50 µL of the solutions containing 4 µg of PIC were mixed with 5 µL of a 6× DNA gel-loading buffer

• with 10% of FBS and 1% of penicillin solution. The cells were kept at 37 °C under 5% CO2 and were subcultured three times per week with 0.25% trypsin/EDTA. The cells were seeded onto 96-well microtiter plates (Greiner) at a concentration of 5 × 103 cells/well (HeLa and MCF7) and 10 × 103 cells/well

Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles

• Sadaf Mushtaq,
• Khuram Shahzad,
• Tariq Saeed,
• Anwar Ul-Hamid,
• Bilal Haider Abbasi,
• Nafees Ahmad,
• Waqas Khalid,
• Muhammad Atif,
• Zulqurnain Ali and
• Rashda Abbasi

Beilstein J. Nanotechnol. 2021, 12, 1339–1364, doi:10.3762/bjnano.12.99

• . Poly(isobutylene-alt-maleic anhydride) (85%), dodecylamine (99%), tetrahydrofuran (THF) (99.9%), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (98%), doxorubicin (99.9%), methotrexate (99.9%), Tris/borate/EDTA buffer (TBE, 98.8%), Dulbecco’s Modified Eagle’s Medium (DMEM, 99.9%), Roswell Park Memorial

• Institute (RPMI-1640, 99.9%) medium, GPPS (2 mM ʟ-glutamine, 1 mM Na pyruvate, 100 U/mL penicillin, 100 µg/mL streptomycin, 98%), Triton X-100 (lab grade), Trizma base, trypsin/EDTA (lab grade), sulforhodamine B (dye content 75%), ethidium bromide (lab grade), sodium dodecyl sulfate (SDS, lab grade), 2',7

• ) with 10% CO2. The cells were harvested using trypsin/EDTA (0.5 mM) for 1 min at room temperature. In vitro cytotoxicity screening of drug-loaded MFe2O4 (M = Fe, Co, Ni, Zn) NPs The cytotoxicity screening of colloidal drug-loaded MFe2O4 (M = Fe, Co, Ni, Zn) NPs was performed by using the SRB assay in

pH-driven enhancement of anti-tubercular drug loading on iron oxide nanoparticles for drug delivery in macrophages

• Karishma Berta Cotta,
• Sarika Mehra and
• Rajdip Bandyopadhyaya

Beilstein J. Nanotechnol. 2021, 12, 1127–1139, doi:10.3762/bjnano.12.84

• /nanoparticle treatment the cells were trypsinized using 1X trypsin-EDTA (HiMedia, India). The cell counts of the trypsinized cells were then determined using the trypan blue assay. Briefly, a fixed volume of animal cell sample was taken and diluted using a 0.4% solution of trypan blue (HiMedia, India). The

Silver nanoparticles induce the cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells via telomere length extension

• Khosro Adibkia,
• Ali Ehsani,
• Asma Jodaei,
• Ezzatollah Fathi,
• Raheleh Farahzadi and
• Mohammad Barzegar-Jalali

Beilstein J. Nanotechnol. 2021, 12, 786–797, doi:10.3762/bjnano.12.62

• by PBS and trypsinized with trypsin-EDTA. The 4 × 103 cells were seeded in each well of a 96-well plate with a total volume of 200 μL of cardiomyogenic differentiation medium of different concentrations (0, 1.25, 2.5, and 5 µg/mL) of Ag-NPs and incubated for 14 days at 37 °C in a humidified

Fate and transformation of silver nanoparticles in different biological conditions

• Barbara Pem,
• Marija Ćurlin,
• Darija Domazet Jurašin,
• Valerije Vrček,
• Rinea Barbir,
• Vedran Micek,
• Raluca M. Fratila,
• Jesus M. de la Fuente and
• Ivana Vinković Vrček

Beilstein J. Nanotechnol. 2021, 12, 665–679, doi:10.3762/bjnano.12.53

• puncture into a heparinised tube and the plasma was separated by centrifugation. The kidneys, liver, and brain were carefully removed and washed in ice-cold saline. Each tissue (1 g) was homogenised in 10 mL of PBS (0.05 M, pH 7.4) containing 0.1 mM of EDTA using a motor-driven homogenizer. Both WB and BP

On the stability of microwave-fabricated SERS substrates – chemical and morphological considerations

• Limin Wang,
• Aisha Adebola Womiloju,
• Christiane Höppener,
• Ulrich S. Schubert and
• Stephanie Hoeppener

Beilstein J. Nanotechnol. 2021, 12, 541–551, doi:10.3762/bjnano.12.44

• choice of a suitable media is of utmost importance for many biological investigations as it can alter the structure of biomolecules (e.g., proteins) [33]. We tested five commonly used buffer systems which include phosphate-buffered saline (PBS), HEPES-buffered glucose solution (HBG), tris-borate-EDTA

Doxorubicin-loaded gold nanorods: a multifunctional chemo-photothermal nanoplatform for cancer management

• Uzma Azeem Awan,
• Abida Raza,
• Shaukat Ali,
• Rida Fatima Saeed and
• Nosheen Akhtar

Beilstein J. Nanotechnol. 2021, 12, 295–303, doi:10.3762/bjnano.12.24

• volunteers and used with Institutional Review Board (IRB) bioethics approval. The hemolysis assay was carried out according to the protocol from National Cancer Institute (NCI). Whole blood (5 mL) from two healthy human donors was drawn directly into K2-EDTA-coated tubes to prevent coagulation. Blood

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

• Barbora Svitkova,
• Vlasta Zavisova,
• Veronika Nemethova,
• Martina Koneracka,
• Miroslava Kretova,
• Filip Razga,
• Monika Ursinyova and
• Alena Gabelova

Beilstein J. Nanotechnol. 2021, 12, 270–281, doi:10.3762/bjnano.12.22

• normalization of data. Western blotting Cells were lysed in buffer containing 50 mM Tris/HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, and 1 mM EDTA, supplemented with protease inhibitor mix (Serva, BioTech, Ltd., Slovakia). After determining the protein concentration using the Bradford assay, equal amounts of

Nanocasting synthesis of BiFeO₃ nanoparticles with enhanced visible-light photocatalytic activity

• Thomas Cadenbach,
• Maria J. Benitez,
• A. Lucia Morales,
• Cesar Costa Vera,
• Luis Lascano,
• Francisco Quiroz,
• Alexis Debut and
• Karla Vizuete

Beilstein J. Nanotechnol. 2020, 11, 1822–1833, doi:10.3762/bjnano.11.164

• experiments with different scavengers, such as AgNO3, ethylenediaminetetraacetic acid (EDTA), tert-butyl alcohol (TBA), and benzoquinone (BQ), was performed. It is generally well-accepted that in semiconductor photocatalysis the reaction of promoted electrons with molecular oxygen leads to superoxide anion

• consumption of excited electrons by the electron scavenger AgNO3 and the resulting enhanced separation of electron–hole pairs [60]. Photogenerated electron holes could play an important role in the present reaction, as the addition of the electron-hole scavenger EDTA (2 mM) decreases the degradation

PEG/PEI-functionalized single-walled carbon nanotubes as delivery carriers for doxorubicin: synthesis, characterization, and in vitro evaluation

• Shuoye Yang,
• Zhenwei Wang,
• Yahong Ping,
• Yuying Miao,
• Yongmei Xiao,
• Lingbo Qu,
• Lu Zhang,
• Yuansen Hu and
• Jinshui Wang

Beilstein J. Nanotechnol. 2020, 11, 1728–1741, doi:10.3762/bjnano.11.155

• were obtained from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). Trypsin–EDTA solution (0.25% trypsin with 0.02% EDTA) was supplied by Beyotime Biotechnology (Beijing, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from Sigma-Aldrich (St

• wells, and the cells were trypsinized with trypsin–EDTA and washed with cold PBS for three times to remove the nanocarriers outside the cells. The cells were then collected and resuspended in 200 μL of PBS by centrifugation. The mean fluorescence intensity (MFI) was determined using FCM (BD Biosciences

Transient coating of γ-Fe₂O₃ nanoparticles with glutamate for its delivery to and removal from brain nerve terminals

• Konstantin Paliienko,
• Artem Pastukhov,
• Michal Babič,
• Daniel Horák,
• Olga Vasylchenko and
• Tatiana Borisova

Beilstein J. Nanotechnol. 2020, 11, 1381–1393, doi:10.3762/bjnano.11.122

• and the membrane potential of nerve terminals. Six animals were used for blood plasma preparation. Isolation of nerve terminals (synaptosomes) from rat cortex The cortex zone of the rat brain was rapidly removed and homogenized in ice-cold 0.32 M sucrose, 5 mM HEPES-NaOH, pH 7.4 and 0.2 mM EDTA. The

Identification of physicochemical properties that modulate nanoparticle aggregation in blood

• Ludovica Soddu,
• Duong N. Trinh,
• Eimear Dunne,
• Dermot Kenny,
• Giorgia Bernardini,
• Ida Kokalari,
• Arianna Marucco,
• Marco P. Monopoli and
• Ivana Fenoglio

Beilstein J. Nanotechnol. 2020, 11, 550–567, doi:10.3762/bjnano.11.44

• aggregation. Materials and Methods Reagents Sodium polyacrylate, ᴅ-(+)-glucose, thionine acetate salt, phosphate buffered saline powder, EDTA, glutathione reduced and 5,5′-dithiobis(2-nitrobenzoic acid), tetraethyl orthosilicate (TEOS), phosphate buffered saline (PBS) tablets were obtained from Sigma-Aldrich