Carbon nano-onions (multi-layer fullerenes): chemistry and applications

• Juergen Bartelmess and
• Silvia Giordani

Beilstein J. Nanotechnol. 2014, 5, 1980–1998, doi:10.3762/bjnano.5.207

• evidenced by a reduced secretion of the inflammatory cytokine IL-1β, and a pronounced decrease in the recruitment of neutrophils and monocytes following injection into mice. Subsequently, in two recent studies, we investigated the effects of two different, fluorophore functionalized CNOs on HeLa Kyoto [40

• promising material for biomedical applications. Recently we demonstrated the cellular imaging of HeLa Kyoto [40] and MCF-7 cells [41] after incubating them with azaBODIPY- or BODIPY-functionalized CNOs (Scheme 8 and Figure 7). In both cases the CNO conjugates were readily internalized by the cells. In the

• (green) and nuclei stain Hoechst (blue) (right). Reprinted with permission from [39]. Copyright 2013 John Wiley and Sons. Confocal images of azaBODIPY-CNOs in HeLa Kyoto cells (left) and BODIPY-CNOs in MCF-7 cells (right). The blue luminescence is due to Hoechst 33342 nuclear stain. Reproduced with

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages

• Dagmar A. Kuhn,
• Dimitri Vanhecke,
• Benjamin Michen,
• Fabian Blank,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1625–1636, doi:10.3762/bjnano.5.174

• or macropinocytosis is involved in both the uptake of larger aggregates of 40 nm NPs and 1 µm particles. Our findings are in agreement with other studies that showed a reduced uptake of 40 nm carboxylated polystyrene particles in HeLa and 1321N1 cells in the presence of cytochalasin A [52] and by

Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy

• Adriano A. Torrano and
• Christoph Bräuchle

Beilstein J. Nanotechnol. 2014, 5, 1616–1624, doi:10.3762/bjnano.5.173

• derived from the cervix carcinoma (HeLa). The absolute number of intracellular silica nanoparticles within the first 24 h was determined and shown to be cell type-dependent. As a second case study, Particle_in_Cell-3D was used to assess the uptake kinetics of 8 nm and 30 nm ceria nanoparticles interacting

• . In this section, we want to present in detail how Particle_in_Cell-3D was used to study the cell type-dependent uptake of 310 nm silica nanoparticles into human vascular endothelial cells (HUVEC) and cancer cells derived from the cervix carcinoma (HeLa). The nanoparticle uptake by single cells was

• for HeLa cells. However, after 10 or 24 h of interaction, the amount of particles taken up by HeLa cells strikingly exceeded the amount of silica particles taken up by HUVEC cells. Characterization of silica nanoparticles In order to allow for the investigation with live-cell imaging, silica

Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity

• Dickson Joseph,
• Nisha Tyagi,
• Christian Geckeler and
• Kurt E.Geckeler

Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158

• prepared AuNPs as an effective anticancer agent, MTT assays were performed against three different cancer cells lines (human colorectal cancer cells (HCT116), human cervical cancer cells (HeLa) and squamous carcinoma cells (SCC-7)) by treating them with AuNPs. The results are shown in Figure 4B, 4C and 4D

• µg/mL and 32.68 µg/mL for Hela, which shows that these nanoparticles cause 50% inhibition of the cancer cell at a lower concentration in comparison to the fibroblasts. This can be attributed due to the difference in the surface charge at different pH conditions. The surface charge on BSA-AuNPs at

• OVA > BGG > LYS > BSA > BHG > HIS, OVA > BGG > LYS > BHG > BSA > HIS and OVA > BGG > BSA > BHG > LYS > HIS for the HCT116, HeLa and SCC-7 cell lines, respectively (Table S3, Supporting Information File 1). The MTT assay on the blank proteins showed cell viabilities greater than 80% (Figure S7

Nanodiamond-DGEA peptide conjugates for enhanced delivery of doxorubicin to prostate cancer

• Amanee D Salaam,
• Patrick Hwang,
• Roberus McIntosh,
• Hadiyah N Green,
• Ho-Wook Jun and
• Derrick Dean

Beilstein J. Nanotechnol. 2014, 5, 937–945, doi:10.3762/bjnano.5.107

• ]. Liang et al. demonstrated that DOX-loaded micelles can efficiently use the tumor-targeting function of RGD sequence to deliver the drug into HeLa cells [38]. Tian et al. showed that iRGD exosomes delivered DOX specifically to tumor tissues and inhibited tumor growth without overt toxicity [39]. Zhou et

Nanolesions induced by heavy ions in human tissues: Experimental and theoretical studies

• Marcus Bleicher,
• Lucas Burigo,
• Marco Durante,
• Maren Herrlitz,
• Michael Krämer,
• Igor Mishustin,
• Iris Müller,
• Francesco Natale,
• Igor Pshenichnov,
• Stefan Schramm,
• Gisela Taucher-Scholz and
• Cathrin Wälzlein

Beilstein J. Nanotechnol. 2012, 3, 556–563, doi:10.3762/bjnano.3.64

• , for long-term live-cell observations. Image acquisition is done by a Hamamatsu C7190 EB-CCD camera. Photobleaching of GFP-tagged H2B in living HeLa cells by the 405 laser is demonstrated in Figure 9. By turning and panning of the laser circle, the logo of the Beilstein-Institut was visualized by

• deflection of the primary ions leads to a natural "diffusion" of the radial dose. Living HeLa cells expressing histone H2B tagged to GFP were photobleached. Bleaching within a region of three sectors of a circle depletes fluorescence from the bleached region. Colors of the three regions were adjusted with