Nanolesions induced by heavy ions in human tissues: Experimental and theoretical studies

• Marcus Bleicher,
• Lucas Burigo,
• Marco Durante,
• Maren Herrlitz,
• Michael Krämer,
• Igor Mishustin,
• Iris Müller,
• Francesco Natale,
• Igor Pshenichnov,
• Stefan Schramm,
• Gisela Taucher-Scholz and
• Cathrin Wälzlein

Beilstein J. Nanotechnol. 2012, 3, 556–563, doi:10.3762/bjnano.3.64

• , for long-term live-cell observations. Image acquisition is done by a Hamamatsu C7190 EB-CCD camera. Photobleaching of GFP-tagged H2B in living HeLa cells by the 405 laser is demonstrated in Figure 9. By turning and panning of the laser circle, the logo of the Beilstein-Institut was visualized by

• deflection of the primary ions leads to a natural "diffusion" of the radial dose. Living HeLa cells expressing histone H2B tagged to GFP were photobleached. Bleaching within a region of three sectors of a circle depletes fluorescence from the bleached region. Colors of the three regions were adjusted with