Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells

• Christa Schimpel,
• Oliver Werzer,
• Eleonore Fröhlich,
• Gerd Leitinger,
• Markus Absenger-Novak,
• Birgit Teubl,
• Andreas Zimmer and
• Eva Roblegg

Beilstein J. Nanotechnol. 2015, 6, 1457–1466, doi:10.3762/bjnano.6.151

• the differentiation of M cells. Cell monolayer integrity and confluence were evaluated by measuring the transepithelial electrical resistance (TEER) with an Endohm culture cup connected to an epithelial volt ohm meter (World Precisions Instruments, Sarasota, USA). For AFM cell imaging/force

Hollow plasmonic antennas for broadband SERS spectroscopy

• Gabriele C. Messina,
• Mario Malerba,
• Pierfrancesco Zilio,
• Ermanno Miele,
• Michele Dipalo,
• Lorenzo Ferrara and
• Francesco De Angelis

Beilstein J. Nanotechnol. 2015, 6, 492–498, doi:10.3762/bjnano.6.50

• ][18] and magnetic field enhancement [19]. In these various disciplines, the rise of a trend targeting high performance spectroscopy techniques for biomolecules and cells can be recognized. Raman spectroscopy has already been implemented for whole live cell imaging [20] as well as its biological

A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes

• Florian G. Strobl,
• Dominik Breyer,
• Phillip Link,
• Adriano A. Torrano,
• Christoph Bräuchle,
• Matthias F. Schneider and
• Achim Wixforth

Beilstein J. Nanotechnol. 2015, 6, 414–419, doi:10.3762/bjnano.6.41

• either a sample observation through the piezoelectric substrate or a coupling of the acoustic power into the sample chamber. This means a loss of either optical quality or energetic efficiency. The L-shape structure of this setup allows for the application of standard cell imaging slides while directly

Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating

• Christina Rosman,
• Sebastien Pierrat,
• Marco Tarantola,
• David Schneider,
• Eva Sunnick,
• Andreas Janshoff and
• Carsten Sönnichsen

Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257

• biocompatible polymer exhibiting one of two different end groups, resulting in a neutral or negative surface charge of the particle. Upon observation of cell growth for three days by live cell imaging using optical dark field microscopy, it was found that all particles supported cell adhesion while no directed

• . Live cell imaging was performed over the course of an incubation time of three days using optical dark field microscopy in order to evaluate the cell adhesion and spreading by the cell morphology. We observe an influence of the particle coating on the growth behavior with respect to the cytotoxic

• obtained from gel electrophoresis for PEG particles can be found in the Supporting Information of [20]. Live cell imaging In order to investigate how single MDCK II cells adhere and grow on nanoparticle-decorated substrates, live cell imaging using optical dark field microscopy was performed. For

Nanoparticle interactions with live cells: Quantitative fluorescence microscopy of nanoparticle size effects

• Li Shang,
• Karin Nienhaus,
• Xiue Jiang,
• Linxiao Yang,
• Katharina Landfester,
• Volker Mailänder,
• Thomas Simmet and
• G. Ulrich Nienhaus

Beilstein J. Nanotechnol. 2014, 5, 2388–2397, doi:10.3762/bjnano.5.248

• ; cationic and NPS, 7.5 µg/mL). AuNCs were suspended in serum-free DMEM at 20 µg/mL. Live cell imaging was performed for up to 2 h with our spinning disk laser scanning confocal microscopy systems [29][31]. Images were acquired in two separate color channels. NP emission was collected through a bandpass

Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells

• Michal Babič,
• Daniel Horák,
• Lyubov L. Lukash,
• Tetiana A. Ruban,
• Yurii N. Kolomiets,
• Svitlana P. Shpylova and
• Oksana A. Grypych

Beilstein J. Nanotechnol. 2014, 5, 1732–1737, doi:10.3762/bjnano.5.183

• only D-mannose- and PDMAAm-coated γ-Fe2O3 particles were internalized by the cells and subsequently found then in the cytoplasm. These nanoparticles can thus serve as potential probes for cell imaging. In particular, PDMAAm proved to be a highly efficient coating providing several attractive properties

Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages

• Dagmar A. Kuhn,
• Dimitri Vanhecke,
• Benjamin Michen,
• Fabian Blank,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1625–1636, doi:10.3762/bjnano.5.174

• within only a few minutes in both cell types (Figure 5). These observations are supported by live cell imaging, which revealed that NP uptake is a very fast process, starting 5 to 10 minutes after exposure to the cells (Figures S2 and S3, Supporting Information File 1). Uptake of 1 µm particles was only

• glass slide. The experiment was performed using a 63x/N.A 1.4 immersion oil lens. Cellular and morphological information was retrieved using Imaris software (Bitplane 7.4, Zürich, Switzerland). For live cell imaging, the cells were seeded in a Lab-TekTM II chambered coverglass 4 chamber well (1.5 german

• phenol red pH indicator) was added with either 40 nm or 1 µm polystyrene particles alone or in combination with the inhibitor, and time lapse imaging was started. The live cell imaging ran over a time period of 60 minutes during which the cells were kept in a constant environmental at 37 °C and 5% CO2

Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy

• Adriano A. Torrano and
• Christoph Bräuchle

Beilstein J. Nanotechnol. 2014, 5, 1616–1624, doi:10.3762/bjnano.5.173

• nanoparticles. The flow is generated by a novel microfluidic reactor that can be combined with live-cell imaging and is able to cover the entire physiological range of shear rates [31]. Comparison to other methods Customary techniques performed for achieving the dosage of particles taken up by cells include

• for HeLa cells. However, after 10 or 24 h of interaction, the amount of particles taken up by HeLa cells strikingly exceeded the amount of silica particles taken up by HUVEC cells. Characterization of silica nanoparticles In order to allow for the investigation with live-cell imaging, silica

• Technologies) and 1 µg·mL−1 hydrocortisone (Sigma-Aldrich). Cells were kept in a humidified 5% CO2 atmosphere at 37 °C. Uptake experiments For live-cell imaging experiments, cells were seeded 24 h before imaging in 8-well Nunc™ Lab-Tek™ II chamber slides (Thermo Fisher Scientific Inc., Germany) at a density of

Synthesis of hydrophobic photoluminescent carbon nanodots by using L-tyrosine and citric acid through a thermal oxidation route

• Venkatesh Gude

Beilstein J. Nanotechnol. 2014, 5, 1513–1522, doi:10.3762/bjnano.5.164

• proline are used as source for producing hydrophilic CNDs in the presence of acid or alkali through microwave pyrolysis [22]. A survey of the literature revealed that the majority of the reports deals with the fabrication of hydrophilic CNDs and their use in cell-imaging [23], sensor [24][25][26][27

Optimizing the synthesis of CdS/ZnS core/shell semiconductor nanocrystals for bioimaging applications

• Li-wei Liu,
• Si-yi Hu,
• Ying Pan,
• Jia-qi Zhang,
• Yue-shu Feng and
• Xi-he Zhang

Beilstein J. Nanotechnol. 2014, 5, 919–926, doi:10.3762/bjnano.5.105

• -encapsulated nanoparticles under various pH values, testing the stability of the F127-CdS/ZnS QDs. The variation of the hydrodynamic diameter under different pH values is shown in Figure 9. The result suggests that their stability is not affected by pH values. Cell imaging and viability studies For in vitro

• stabilize nanospheres and prolong their circulation time in vivo. In this experiment, F127 was used to modify the surface of the CdS/ZnS QDs Preparation of in vitro cell imaging Panc-1 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum

• (FBS, Hyclone) and cultured at 37 °C in a humidified atmosphere with 5% CO2. For cell imaging, cells were treated with F127-CdS/ZnS micelles formulations for 2 h. The cells were then washed with PBS for three times and imaged using a Leica confocal microscopy system (TCS SP2). For the cell viability

In vitro toxicity and bioimaging studies of gold nanorods formulations coated with biofunctional thiol-PEG molecules and Pluronic block copolymers

• Tianxun Gong,
• Douglas Goh,
• Malini Olivo and
• Ken-Tye Yong

Beilstein J. Nanotechnol. 2014, 5, 546–553, doi:10.3762/bjnano.5.64

• for applications ranging from cell imaging to targeted in vivo drug delivery. Normalized UV–vis absorption spectra of as-synthesized AuNRs and AuNRs washed one, two , three and four times by centrifugation. TEM images of (a) as-synthesized AuNRs, (b) AuNRs washed 4 times by centrifugation, (c

Near-infrared dye loaded polymeric nanoparticles for cancer imaging and therapy and cellular response after laser-induced heating

• Tingjun Lei,
• Alicia Fernandez-Fernandez,
• Romila Manchanda,
• Yen-Chih Huang and
• Anthony J. McGoron

Beilstein J. Nanotechnol. 2014, 5, 313–322, doi:10.3762/bjnano.5.35

• cells could result in the promotion of HIF-1 expression after incubator induced-HT. The VEGF secretion was also significantly enhanced compared to laser/NP HT, possibly due to the promotion of HIF-1. In vitro cell imaging and in vivo healthy mice imaging showed that IR820-PGMD NPs can be used for

Nanolesions induced by heavy ions in human tissues: Experimental and theoretical studies

• Marcus Bleicher,
• Lucas Burigo,
• Marco Durante,
• Maren Herrlitz,
• Michael Krämer,
• Igor Mishustin,
• Iris Müller,
• Francesco Natale,
• Igor Pshenichnov,
• Stefan Schramm,
• Gisela Taucher-Scholz and
• Cathrin Wälzlein

Beilstein J. Nanotechnol. 2012, 3, 556–563, doi:10.3762/bjnano.3.64

• be linear. However, using a double-strand break (DSB)-specific marker (phosphorylated histone γH2AX), we found “bending” of the streaks when cells were fixed for 30 min or more after irradiation [8] (Figure 1). Reconstruction of the track dynamics by using live-cell imaging (Supporting Information

• nanolesions in tissues. Further extensions of the MCHIT and TRAX code will be necessary to obtain a satisfactory description of energy deposition and track behavior at the nanometer scale in realistic targets. Experimental Detailed experimental methods for immunohistochemistry and live-cell imaging in our