Antibody-conjugated nanoparticles for target-specific drug delivery of chemotherapeutics

• Mamta Kumari,
• Amitabha Acharya and
• Praveen Thaggikuppe Krishnamurthy

Beilstein J. Nanotechnol. 2023, 14, 912–926, doi:10.3762/bjnano.14.75

• specifically with antigens, which are responsible for the production or induction of specific antibodies [17]. The specific binding of antigens to their receptors activates a signaling pathway in B-cells, which leads to the secretion of antibodies into biological fluids [18]. Antibodies have four polypeptide

Nanotechnology – a robust tool for fighting the challenges of drug resistance in non-small cell lung cancer

• Filip Gorachinov,
• Fatima Mraiche,
• Diala Alhaj Moustafa,
• Ola Hishari,
• Yomna Ismail,
• Jensa Joseph,
• Maja Simonoska Crcarevska,
• Marija Glavas Dodov,
• Nikola Geskovski and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2023, 14, 240–261, doi:10.3762/bjnano.14.23

• copolymer core–shell NPs, (ii) polymer–polypeptide hybrid core–shell NPs, and (iii) polymer–lipid hybrid core–shell NPs additionally decorated with ligands for overexpressed receptors on cancer cells [92]. Traditionally selected overexpressed cancer cell surface markers for the active targeting of NPs

DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection

• Nathalie B. F. Almeida,
• Thiago A. S. L. Sousa,
• Viviane C. F. Santos,
• Camila M. S. Lacerda,
• Thais G. Silva,
• Rafaella F. Q. Grenfell,
• Flavio Plentz and
• Antero S. R. Andrade

Beilstein J. Nanotechnol. 2022, 13, 873–881, doi:10.3762/bjnano.13.78

• replication [4]. The glycoprotein NS1 is a 352-amino-acid polypeptide, which has a molecular weight of 46–55 kDa (depending on its glycosylation status), and is essential in viral replication contributing to different stages of the virus life cycle. The NS1 protein is found at high levels in the blood of

Stimuli-responsive polypeptide nanogels for trypsin inhibition

• Petr Šálek,
• Jana Dvořáková,
• Sviatoslav Hladysh,
• Diana Oleshchuk,
• Ewa Pavlova,
• Jan Kučka and
• Vladimír Proks

Beilstein J. Nanotechnol. 2022, 13, 538–548, doi:10.3762/bjnano.13.45

• Science, Charles University, Hlavova 8, 128 40 Prague 2, Czech Republic 10.3762/bjnano.13.45 Abstract A new type of hydrophilic, biocompatible, and biodegradable polypeptide nanogel depots loaded with the natural serine protease inhibitor α1-antitrypsin (AAT) was applied for the inhibition of the

• trypsin because they contain suitable amino acids in their structures that effectively block the active site of trypsin. Keywords: α1-antitrypsin; inflammatory mediator; nanogel; polypeptide; trypsin; Introduction Despite significant and progressive advances in medicine, the global incidence of

• -(2-hydroxyethyl)-ᴅʟ-aspartamide-ran-(2-(4-hydroxyphenyl)ethyl)-ᴅʟ-aspartamide] (Nα-Lys-NG) and poly[N5-(2-hydroxyethyl)-ʟ-glutamine-ran-N5-propargyl-ʟ-glutamine-ran-N5-(6-aminohexyl)-ʟ-glutamine]-ran-N5-[2-(4-hydroxyphenyl)ethyl)-ʟ-glutamine] (PHEG-Tyr) polypeptide precursors by HRP/H2O2-mediated

The impact of molecular tumor profiling on the design strategies for targeting myeloid leukemia and EGFR/CD44-positive solid tumors

• Nikola Geskovski,
• Nadica Matevska-Geshkovska,
• Simona Dimchevska Sazdovska,
• Marija Glavas Dodov,
• Kristina Mladenovska and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2021, 12, 375–401, doi:10.3762/bjnano.12.31

A 3D-polyphenylalanine network inside porous alumina: Synthesis and characterization of an inorganic–organic composite membrane

• Jonathan Stott and
• Jörg J. Schneider

Beilstein J. Nanotechnol. 2020, 11, 938–951, doi:10.3762/bjnano.11.78

• -dimensional fibrillar network with intrinsic porosity was formed in DCM, whereas in THF, a dense and smooth polypeptide film was observed. A post-treatment with a mixture of chloroform and dichloroacetic acid leads to rearrangement of the morphology of the grafted polymer films. The analysis by scanning

• applications. The most common route to graft polypeptides on surfaces is the surface-initiated ring-opening polymerization (SI-ROP) of α-aminoacid-N-carboxyanhydrides (NCAs). Dense polypeptide films consisting of poly(γ-benzyl-ʟ-glutamate), polylysine or polyphenylalanine with thickness in the range between

• several nanometers to over 100 nanometers were realized on flat surfaces [9][10][11][12][13][14]. Whitesell et al. first described a SI-ROP of alanine-NCA and phenylalanine-NCA on flat surfaces [15]. In the last decades poly(γ-benzyl-ʟ-glutamate) has become the most prominent polypeptide with respect to

Self-assembly of chiral fluorescent nanoparticles based on water-soluble L-tryptophan derivatives of p-tert-butylthiacalix[4]arene

• Pavel L. Padnya,
• Irina A. Khripunova,
• Olga A. Mostovaya,
• Timur A. Mukhametzyanov,
• Vladimir G. Evtugyn,
• Vyacheslav V. Vorobev,
• Yuri N. Osin and
• Ivan I. Stoikov

Beilstein J. Nanotechnol. 2017, 8, 1825–1835, doi:10.3762/bjnano.8.184

• , probably does not contribute to excimer formation. It is known that circular dichroism (CD) spectroscopy is widely used for the analysis of the secondary structure of proteins [58]. It is possible to assume exactly how the molecule of the linear polypeptide is packed in space by the form of the CD spectrum

Functional diversity of resilin in Arthropoda

• Jan Michels,
• Esther Appel and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2016, 7, 1241–1259, doi:10.3762/bjnano.7.115

• exoskeletons of arthropods. It is composed of randomly orientated coiled polypeptide chains that are covalently cross-linked together at regular intervals by the two unusual amino acids dityrosine and trityrosine forming a stable network with a high degree of flexibility and mobility. As a result of its

• compilation of the then existing knowledge of resilin properties [5]. Resilin consists of a network of randomly orientated coiled polypeptide chains that have a high degree of flexibility and mobility and are linked together at regular intervals by stable covalent cross-links. Only the fully cross-linked

• and trityrosine, which were identified to form the cross-links between the polypeptide chains [9]. The mechanical properties of resilin strongly depend on the degree of hydration because resilin is plasticised by water [5]. When resilin is completely hydrated, it behaves close to a perfect rubber [3

Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles

• Igor M. Pongrac,
• Marina Dobrivojević,
• Lada Brkić Ahmed,
• Michal Babič,
• Miroslav Šlouf,
• Daniel Horák and
• Srećko Gajović

Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84

• , since it is highly biocompatible, easy to use, available on the market and promotes internalization with highly efficiency, e.g., into human mesenchymal stem cells [18][19]. As a positively charged polypeptide, PLL is used for nonspecific adhesion of cells to solid substrates through enhancing

Single-molecule mechanics of protein-labelled DNA handles

• Vivek S. Jadhav,
• Dorothea Brüggemann,
• Florian Wruck and
• Martin Hegner

Beilstein J. Nanotechnol. 2016, 7, 138–148, doi:10.3762/bjnano.7.16

• ) in high-resolution measurements [10]. We previously presented optical force rupture measurements of the nascent polypeptide chain from biotinylated ribosomes, which were specifically bound to surface-modified polystyrene beads [11]. While the ribosome was translating, the biotinylated lysine residue

• at the beginning of the nascent polypeptide chain was labelled in situ with streptavidin. This arrangement enabled us to grasp the protein chain with a DIG-DNA-Bio handle of 4056 bp length. Although we were able to study the rupture forces using this approach, the labelling of the nascent proteins

• is shown in Figure 7d. Thus, PDH constructs could be employed in such a dual-handle configuration to grasp doubly biotinylated molecules (or ensembles of molecules) in situ, for instance to tether a biotinylated ribosome exposing a stalled biotinylated nascent polypeptide chain. Using 0.84 μm beads

Peptide-equipped tobacco mosaic virus templates for selective and controllable biomineral deposition

• Klara Altintoprak,
• Axel Seidenstücker,
• Alexander Welle,
• Sabine Eiben,
• Petia Atanasova,
• Nina Stitz,
• Alfred Plettl,
• Joachim Bill,
• Hartmut Gliemann,
• Holger Jeske,
• Dirk Rothenstein,
• Fania Geiger and
• Christina Wege

Beilstein J. Nanotechnol. 2015, 6, 1399–1412, doi:10.3762/bjnano.6.145

• residues, i.e. lysine–aspartic acid (KD)x motifs expected to act as charge-relay systems promoting TEOS hydrolysis and silica polymerization; (ii) a tetrahistidine-exposing polypeptide (CA4H4) known to induce silicification due to the positive charge of its clustered imidazole side chains; and (iii) two

Functionalization of α-synuclein fibrils

• Simona Povilonienė,
• Vida Časaitė,
• Virginijus Bukauskas,
• Arūnas Šetkus,
• Juozas Staniulis and
• Rolandas Meškys

Beilstein J. Nanotechnol. 2015, 6, 124–133, doi:10.3762/bjnano.6.12

• self-assembled structures, amyloid proteins have become a very attractive material in the field of nanobiotechnology [1]. Many proteins or peptides can form amyloids under appropriate experimental conditions and recent studies suggest that amyloid formation is a generic property of the polypeptide

Biopolymer colloids for controlling and templating inorganic synthesis

• Laura C. Preiss,
• Katharina Landfester and
• Rafael Muñoz-Espí

Beilstein J. Nanotechnol. 2014, 5, 2129–2138, doi:10.3762/bjnano.5.222

• prepared through a polypeptide-templated synthesis by using the interactions of a polypeptide of L-lysine with silicate [35]. Conclusion The application of biopolymers (polysaccharides, peptides, and nucleic acids) as controlling agents or as templates of inorganic precipitation and crystallization is not

Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity

• Dickson Joseph,
• Nisha Tyagi,
• Christian Geckeler and
• Kurt E.Geckeler

Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158

• eight selected proteins in Table 1. Second, because HG exists as a single complex with a molecular weight greater than 60,000 g/mol in its natural state and its four polypeptide chains are separated (each with molecular weights of approximately 17,000 g/mol) in its denatured state, thus, the natural and

Model systems for studying cell adhesion and biomimetic actin networks

• Dorothea Brüggemann,
• Johannes P. Frohnmayer and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131

• interacts simultaneously via its 47 kDa polypeptide domain with lipid bilayers and via its 200 kDa domain with actin [79]. Moreover, talin was found to trigger vesicle membrane fusion, which could be monitored using cryoelectron microscopy [80]. Hence, talin is of major importance for understanding

Oriented attachment explains cobalt ferrite nanoparticle growth in bioinspired syntheses

• Annalena Wolff,
• Walid Hetaba,
• Marco Wißbrock,
• Stefan Löffler,
• Nadine Mill,
• Katrin Eckstädt,
• Axel Dreyer,
• Inga Ennen,
• Norbert Sewald,
• Peter Schattschneider and
• Andreas Hütten

Beilstein J. Nanotechnol. 2014, 5, 210–218, doi:10.3762/bjnano.5.23

• diffraction measurements. The change of particle diameter with time agrees with the recently proposed kinetic model for oriented attachment. Keywords: bioinspired synthesis; cobalt ferrite nanoparticles; nanoparticle growth; oriented attachment; polypeptide; Introduction Nanoparticles with a well-controlled

• oriented attachment can be separated into the following four steps: Crystallite formation Polypeptide-nanoparticle interaction and growth of primary building blocks (pbb) Mesocrystals: aggregation and orientation of primary building blocks Formation of secondary nanoparticles Only the growth of the cobalt

• ferrite nanoparticles was studied in this work. A detailed description of the crystallite formation is therefore not discussed here. Polypeptide-nanoparticle interaction and growth of primary building blocks The driving force of crystal growth is the reduction of surface energy. The final crystal faces

Controlled synthesis and tunable properties of ultrathin silica nanotubes through spontaneous polycondensation on polyamine fibrils

• Jian-Jun Yuan,
• Pei-Xin Zhu,
• Daisuke Noda and
• Ren-Hua Jin

Beilstein J. Nanotechnol. 2013, 4, 793–804, doi:10.3762/bjnano.4.90

• of roughly 3 nm. While self-assembled polypeptides could template the formation of silica nanotubes with uniform diameter, it still remains challenge for the polypeptide template to synthesize stable silica nanotube structures with very small diameters (i.e., 10 nm) [21][22][23][24][25][26][27

Review and outlook: from single nanoparticles to self-assembled monolayers and granular GMR sensors

• Alexander Weddemann,
• Inga Ennen,
• Anna Regtmeier,
• Camelia Albon,
• Annalena Wolff,
• Katrin Eckstädt,
• Nadine Mill,
• Michael K.-H. Peter,
• Jochen Mattay,
• Carolin Plattner,
• Norbert Sewald and
• Andreas Hütten

Beilstein J. Nanotechnol. 2010, 1, 75–93, doi:10.3762/bjnano.1.10

• hydrophilic. The hydrophilic C-terminal of Mms6 is believed to be the iron binding site [26]. Recent studies within our group showed that nanoparticles can be synthesized in vitro by the use of a shorter synthetic version of the protein Mms6 called c25-mms6. This polypeptide consists of 25 amino acids from

Sensing surface PEGylation with microcantilevers

• Natalija Backmann,
• Natascha Kappeler,
• Thomas Braun,
• François Huber,
• Hans-Peter Lang,
• Christoph Gerber and
• Roderick Y. H. Lim

Beilstein J. Nanotechnol. 2010, 1, 3–13, doi:10.3762/bjnano.1.2

• biotechnology on account of its good solubility in water and low toxicity. Polypeptide drugs and nanoparticles designed for drug delivery exhibit enhanced biocompatibility and proteolytic resistance (e.g., “stealth” particles) when modified with PEG (i.e., PEGylation) [1][2]. PEG-functionalized surfaces are