Classification and application of metal-based nanoantioxidants in medicine and healthcare

• Nguyen Nhat Nam,
• Nguyen Khoi Song Tran,
• Tan Tai Nguyen,
• Nguyen Ngoc Trai,
• Nguyen Phuong Thuy,
• Hoang Dang Khoa Do,
• Nhu Hoa Thi Tran and
• Kieu The Loan Trinh

Beilstein J. Nanotechnol. 2024, 15, 396–415, doi:10.3762/bjnano.15.36

• strongly to high levels of ROS that can cause damage to DNA molecules resulting in abnormal cell proliferation. Various studies reported that oxidative damage can have a deleterious effect on cancer development through raising genetic mutations, abnormal protein functions, and tumor growth [164][165

Exploring the relationships between physiochemical properties of nanoparticles and cell damage to combat cancer growth using simple periodic table-based descriptors

• Joyita Roy and
• Kunal Roy

Beilstein J. Nanotechnol. 2024, 15, 297–309, doi:10.3762/bjnano.15.27

• systems. Metal NPs can lead to greater signal amplification, greater sensitivity, and higher detection. However, NPs with properties that generate ROS can increase cell damage. In cancer cells, rapid proliferation leads to an imbalance of oxygen, abnormal structure, and blood supply, making the tumor

Vinorelbine-loaded multifunctional magnetic nanoparticles as anticancer drug delivery systems: synthesis, characterization, and in vitro release study

• Zeynep Özcan and
• Afife Binnaz Hazar Yoruç

Beilstein J. Nanotechnol. 2024, 15, 256–269, doi:10.3762/bjnano.15.24

• uncontrolled proliferation of aberrant cells, which can spread to diverse body regions, encompassing over a hundred distinct forms [1][2]. Current cancer treatments lack a complete approach, as they mostly rely on radiotherapy, chemotherapy, immunotherapy, and surgery in clinical environments [3]. While these

• cancer cell growth, slowing their proliferation and disrupting mitotic regulation, leading to the stimulation of the tumor suppressor gene p53 and the inhibition or inactivation of various signaling pathways [31][32]. Its widespread adoption in medicine can be attributed to its strong therapeutic

Berberine-loaded polylactic acid nanofiber scaffold as a drug delivery system: The relationship between chemical characteristics, drug-release behavior, and antibacterial efficiency

• Le Thi Le,
• Hue Thi Nguyen,
• Liem Thanh Nguyen,
• Huy Quang Tran and
• Thuy Thi Thu Nguyen

Beilstein J. Nanotechnol. 2024, 15, 71–82, doi:10.3762/bjnano.15.7

• cytotoxicity test revealed that the BBR NPs/PLA nanofiber scaffold did not induce any changes in morphology and proliferation of MA-104 cell monolayers. It suggests that the BBR/PLA and BBR NPs/PLA nanofiber scaffolds can be used in different biomedical applications, such as wound dressing, drug delivery

• , nanofiber scaffolds can act as a multifunctional tool in medical treatments, combining drug release for disease therapy, cell proliferation, wound healing, and antimicrobial effect [21][22][23][24][25]. Nanofibers of PLA functionalized with laponite (LAP)/amoxicillin (AMX) prolonged the drug release up to

• phases. The exponential phase occurred in the first 12 h when the cell numbers were doubled after each generation time. After that, the stationary phase was reached when the number of growth cells was almost equal to that of dead cells. The proliferation of MRSA incubated in the nutrient broth with the

Curcumin-loaded nanostructured systems for treatment of leishmaniasis: a review

• Douglas Dourado,
• Thayse Silva Medeiros,
• Éverton do Nascimento Alencar,
• Edijane Matos Sales and
• Fábio Rocha Formiga

Beilstein J. Nanotechnol. 2024, 15, 37–50, doi:10.3762/bjnano.15.4

• reactive oxygen/nitrogen metabolites, (ii) increased phagocytic activity, and (iii) increased lymphocyte proliferation [45]. Furthermore, curc-E-PLGA-NPs proved to be effective as an adjuvant in the therapy against leishmaniasis. Like curc, other leishmanicidal drugs have been encapsulated into polymeric

Hierarchically patterned polyurethane microgrooves featuring nanopillars or nanoholes for neurite elongation and alignment

• Lester Uy Vinzons,
• Guo-Chung Dong and
• Shu-Ping Lin

Beilstein J. Nanotechnol. 2023, 14, 1157–1168, doi:10.3762/bjnano.14.96

• compartments, such as the nucleus, filopodia, and focal adhesions, resulting in the modulation of signal cascades that leads to changes in cell proliferation, attachment, orientation, and differentiation, among others [2]. In nerve tissue engineering, the implant micro- and nanotopography serve as physical

• ], poly(lactic-co-glycolic acid) nanodots enhanced the proliferation and neurite sprouting of Neuro-2a cells [7], and oriented elliptical Si microcones induced alignment and increased fasciculation in rat superior cervical ganglion axons [8]. With their effects complementing those of continuous structures

• showed good viability and proliferation on the nanostructured PU films, especially on the PU nanoholes (Supporting Information File 1, Figure S6). After differentiation, PC12 cells extended beta-III tubulin positive neurites, with longer neurites appearing on the nanostructured substrates (Figure 2A–C

Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

• Nittiya Suwannasom,
• Netsai Sriaksorn,
• Chutamas Thepmalee,
• Krissana Khoothiam,
• Ausanai Prapan,
• Hans Bäumler and
• Chonthida Thephinlap

Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93

• especially anticancer potential [1][2]. Several in vivo and in vitro studies in recent years have demonstrated that CUR can influence cancer cell proliferation, invasion, angiogenesis, and metastasis [3]. It has been reported that CUR exerts anticancer effects in human breast cancer cells (MCF-7) by

• stability and solubility [9]. The complexation occurs mainly through hydrophobic interactions in protein cavities [10][11]. In a recent study, zein nanoparticles loaded with CUR have been studied for their potential in treating brain tumors, and the results have demonstrated a reduction in the proliferation

• . After 24 h, free CUR significantly decreased cell proliferation from 40% to 6% (Huh-7, Figure 6A) and 50% to 5% (MCF-7, Figure 6C) at concentrations of 12.5 and 100 μg/mL, respectively. However, CUR-HSA-MPs, at an equivalent concentration of CUR, significantly decreased cell proliferation from 51% to 21

Antibody-conjugated nanoparticles for target-specific drug delivery of chemotherapeutics

• Mamta Kumari,
• Amitabha Acharya and
• Praveen Thaggikuppe Krishnamurthy

Beilstein J. Nanotechnol. 2023, 14, 912–926, doi:10.3762/bjnano.14.75

• nanoformulation provided site-specific delivery via receptor-mediated endocytosis and inhibited proliferation and metastasis in tumors that expressed a specific tumor antigen. The use of adaptor molecules to functionalize antibodies resulted in a highly stable conjugation with improved therapeutic efficacy [69

Green SPIONs as a novel highly selective treatment for leishmaniasis: an in vitro study against Leishmania amazonensis intracellular amastigotes

• Brunno R. F. Verçoza,
• Robson R. Bernardo,
• Luiz Augusto S. de Oliveira and
• Juliany C. F. Rodrigues

Beilstein J. Nanotechnol. 2023, 14, 893–903, doi:10.3762/bjnano.14.73

• effects on cell proliferation, infectivity percentage, and ultrastructure. SPIONs were internalized by both parasite stages, randomly distributed in the cytosol and located mainly in membrane-bound compartments. The selectivity index for intracellular amastigotes was more than 240 times higher compared to

• antiproliferative effects of SPIONs in L. amazonensis promastigotes and intracellular amastigotes. Despite being internalized by promastigotes, SPIONs did not affect the cell proliferation of the parasites (Figure 4A). A completely different result was observed for intracellular amastigotes, where the reduction in

Nanoarchitectonics to entrap living cells in silica-based systems: encapsulations with yolk–shell and sepiolite nanomaterials

• Celia Martín-Morales,
• Jorge Fernández-Méndez,
• Pilar Aranda and
• Eduardo Ruiz-Hitzky

Beilstein J. Nanotechnol. 2023, 14, 522–534, doi:10.3762/bjnano.14.43

• to the previous day, that is, there was no cell proliferation, and the escape of cells from the matrix was negligible. In the case of neat yolk–shell encapsulated cells, the cell leakage has been evaluated mixing the particles in the tube and observing an increase in the absorbance of the supernatant

• above the sedimented particles. This way, a “−“ sign indicates no appreciable cell proliferation in the growth medium besides the cells already encapsulated in the yolk–shell microstructures. All these critical features have been studied and compared among the different systems over a course of ten days

Plasmonic nanotechnology for photothermal applications – an evaluation

• A. R. Indhu,
• L. Keerthana and
• Gnanaprakash Dharmalingam

Beilstein J. Nanotechnol. 2023, 14, 380–419, doi:10.3762/bjnano.14.33

• various material phenomena other than bandgap absorption for heat generation in nanoparticles (NPs), leading to a rapid proliferation of materials for the same. For example, organic materials undergo rapid internal relaxation by the PT effect and are often desired in cancer treatment research as they

Quercetin- and caffeic acid-functionalized chitosan-capped colloidal silver nanoparticles: one-pot synthesis, characterization, and anticancer and antibacterial activities

• Akif Hakan Kurt,
• Elif Berna Olutas,
• Fatma Avcioglu,
• Hamza Karakuş,
• Mehmet Ali Sungur,
• Cansu Kara Oztabag and
• Muhammet Yıldırım

Beilstein J. Nanotechnol. 2023, 14, 362–376, doi:10.3762/bjnano.14.31

• acid hydrate, Cell Proliferation Kit II test (Roche). XTT solution was added to each plate at 50 µL and was incubated for 4 h at 37 °C. Then, the absorbance (optical density, OD) of the solution in each plate was read at 450 nm by a spectrophotometer (BiotekELx800, Winooski, VT, USA). Statistical

• and their viability, as it greatly simplifies the procedure for measuring proliferation over MTT, reduces assay time, and increases the sensitivity of the assay [73]. In this study, the dose-dependent cell viabilities of human brain glioblastoma (U-118 MG) and human retinal pigment epithelium (ARPE-19

Recent progress in cancer cell membrane-based nanoparticles for biomedical applications

• Qixiong Lin,
• Yueyou Peng,
• Yanyan Wen,
• Xiaoqiong Li,
• Donglian Du,
• Weibin Dai,
• Wei Tian and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2023, 14, 262–279, doi:10.3762/bjnano.14.24

• being presented to antigen-presenting cells (APCs), will promote the proliferation and infiltration of active T cells in the TME and induce an antigen-specific antitumor response [33][45]. This natural advantage also makes cancer cell membranes useful in nanoimmunotherapy, which can induce specific

• responses after the presentation and delivery of leukemia membrane-associated antigens by APCs [59]. These bionanoparticles showed the advantage of multiple antigens and significantly prolonged survival. MM, a malignant proliferation of plasma cells derived from the bone marrow, is the second most common

Nanotechnology – a robust tool for fighting the challenges of drug resistance in non-small cell lung cancer

• Filip Gorachinov,
• Fatima Mraiche,
• Diala Alhaj Moustafa,
• Ola Hishari,
• Yomna Ismail,
• Jensa Joseph,
• Maja Simonoska Crcarevska,
• Marija Glavas Dodov,
• Nikola Geskovski and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2023, 14, 240–261, doi:10.3762/bjnano.14.23

• proliferation and survival mechanisms on which the cancer cells are heavily dependent. The efficacy of existing small molecules in synergistic combinations for relevant genetic mutations in resistant cancers has been evaluated in many research and clinical studies, with promising results in some types of mutant

• the limitations of the conjugates [68][69]. Cytotoxic or molecular targeting agents with siRNA Targeting homologous mRNA sequences in cells and knockdown of receptors involved in cell survival and proliferation using RNA interference downregulates receptor protein expression, inhibits cell growth, and

• cycle progression and proliferation [78]. Therefore, it is expected that KRAS-mutated tumors would not respond to EGFR TKIs. Patients with KRAS-mutant NSCLC can benefit from direct KRAS inhibitors, such as sotorasib, which lock KRAS in its inactive GDP-bound form. However, a heterogeneous resistance

Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine

• Zoran M. Marković,
• Milica D. Budimir,
• Martin Danko,
• Dušan D. Milivojević,
• Pavel Kubat,
• Danica Z. Zmejkoski,
• Vladimir B. Pavlović,
• Marija M. Mojsin,
• Milena J. Stevanović and
• Biljana M. Todorović Marković

Beilstein J. Nanotechnol. 2023, 14, 165–174, doi:10.3762/bjnano.14.17

• and thermally stable, quasi-spherical, photoluminescent material with very good antibacterial and anticancer properties under visible light irradiation [9][10][11][12][13][14][15][16]. This material has very good biocompatibility, including low dark cytotoxicity and good cell proliferation

• which MRC5 cells were maintained as monolayer cultures, was replaced with undiluted sample extract (100%), and 50% and 25% sample extracts (prepared by diluting sample extracts with fresh RPMI-1640). After 48 h of treatment with sample extracts, cell proliferation was determined using MTT reduction

Antimicrobial and mechanical properties of functionalized textile by nanoarchitectured photoinduced Ag@polymer coating

• Jessica Plé,
• Marine Dabert,
• Helene Lecoq,
• Sophie Hellé,
• Lydie Ploux and
• Lavinia Balan

Beilstein J. Nanotechnol. 2023, 14, 95–109, doi:10.3762/bjnano.14.11

• Biomédecine de Strasbourg, F-67000 Strasbourg, France Université de Strasbourg, Faculté Dentaire, F-67000 Strasbourg, France CNRS, F-67000 Strasbourg, France 10.3762/bjnano.14.11 Abstract The control of microbial proliferation is a constant battle, especially in the medical field where surfaces, equipment

• nanocomposites exhibited remarkable microbial growth inhibitory effects. Keywords: antimicrobial properties; C. albicans fungus; E. coli bacteria; photoinduced functionalized textile; silver/polymer nanomaterials; Introduction The proliferation of microorganisms is a major concern for health organizations

• avoiding planktonic bacteria proliferation by the direct action of silver ions. The capacity of 3 wt % and 5 wt %-loaded textiles to inhibit the growth of E. coli is thought to be related to this mechanism. Concerning the fungus, similarly to bacteria, the amount of loaded silver does not have a

In search of cytotoxic selectivity on cancer cells with biogenically synthesized Ag/AgCl nanoparticles

• Mitzi J. Ramírez-Hernández,
• Mario Valera-Zaragoza,
• Omar Viñas-Bravo,
• Ariana A. Huerta-Heredia,
• Miguel A. Peña-Rico,
• Erick A. Juarez-Arellano,
• David Paniagua-Vega,
• Eduardo Ramírez-Vargas and
• Saúl Sánchez-Valdes

Beilstein J. Nanotechnol. 2022, 13, 1505–1519, doi:10.3762/bjnano.13.124

• % for AgNPs-T80. In other words, at concentrations close to 50 µg/mL, the cytotoxic action of the nanoparticles becomes selective. This result is possibly due to the fact that a higher content of nanoparticles prevents cell proliferation in neoplastic cells. MCF-7 cells are known to overexpress matrix

Hydroxyapatite–bioglass nanocomposites: Structural, mechanical, and biological aspects

• Olga Shikimaka,
• Mihaela Bivol,
• Bogdan A. Sava,
• Marius Dumitru,
• Christu Tardei,
• Beatrice G. Sbarcea,
• Daria Grabco,
• Constantin Pyrtsac,
• Daria Topal,
• Andrian Prisacaru,
• Vitalie Cobzac and
• Viorel Nacu

Beilstein J. Nanotechnol. 2022, 13, 1490–1504, doi:10.3762/bjnano.13.123

• favorable for bone cell proliferation, although a high pH value may be detrimental to optimal osteoblast metabolism [14]. It was shown, for example, that values of pH 7.0–7.5 were optimal for osteoclast differentiation and proliferation [57]. MTT assay and biocompatibility of composites Figure 9 shows the

• probably caused by the more porous structure and, as a result, higher dissolution rate of these composites, inducing an increased ion concentration in the surrounding biological environment, which may be detrimental for cell proliferation. It can be noticed that HAG-1200 ceramics without BG also exhibited

Facile preparation of Au- and BODIPY-grafted lipid nanoparticles for synergized photothermal therapy

• Yuran Wang,
• Xudong Li,
• Haijun Chen and
• Yu Gao

Beilstein J. Nanotechnol. 2022, 13, 1432–1444, doi:10.3762/bjnano.13.118

• -LNPs at the same concentration, indicating the synergistic effects of BDP and Au-LNPs (Figure 5c). Because of the photothermal effects of BDP and Au-LNPs, AB-LNPs with a BDP concentration of 30 μM and a Au concentration of 30 μM presented strong cell proliferation inhibitory effects (90.2%) in

Orally administered docetaxel-loaded chitosan-decorated cationic PLGA nanoparticles for intestinal tumors: formulation, comprehensive in vitro characterization, and release kinetics

• Sedat Ünal,
• Osman Doğan and
• Yeşim Aktaş

Beilstein J. Nanotechnol. 2022, 13, 1393–1407, doi:10.3762/bjnano.13.115

• : chitosan; docetaxel; intestinal tumors; oral drug delivery; PLGA; Introduction Cancer is one of the most common chronic diseases in the world, characterized by the uncontrolled proliferation and spread of cells [1]. To date, effective and safe treatment approaches for cancer treatment have not been fully

• the proliferation of cancer cells [32][33][34]. Its potent activity against a wide spectrum of cancers such as colon cancer, gastric cancer, breast cancer, recurrent ovarian cancer, and non-small cell lung cancer has been elucidated by in vitro and in vivo studies [35]. Its poor water solubility

Biomimetic chitosan with biocomposite nanomaterials for bone tissue repair and regeneration

• Se-Kwon Kim,
• Sesha Subramanian Murugan,
• Pandurang Appana Dalavi,
• Sebanti Gupta,
• Sukumaran Anil,
• Gi Hun Seong and
• Jayachandran Venkatesan

Beilstein J. Nanotechnol. 2022, 13, 1051–1067, doi:10.3762/bjnano.13.92

• oxide, zinc oxide, carbon nanotubes, graphene oxide, and biosilica was developed to improve bone scaffolds for better bone tissue repair and regeneration [11]. In tissue engineering applications, nanoscale topological characteristics influence cell adhesion, survival, proliferation, and differentiation

• their osteoinductive properties [40][41]. Chitosan is combined with several polymeric materials and nanoparticles to mimic the natural function of the bone (Table 1) [42][43]. Chitosan biomaterials enhance the proliferation of osteoblasts and the formation of bone minerals by promoting gene expression

• differentiation in human adipose-derived mesenchymal stem cells. The proliferation of the cells was demonstrated by the alamarBlue test. Further, qRT-PCR analysis showed upregulation of alkaline phosphatase, bone sialoprotein, and osteocalcin gene expression. The Alizarin red S test was carried out by culturing

Bioselectivity of silk protein-based materials and their bio-inspired applications

• Hendrik Bargel,
• Vanessa T. Trossmann,
• Christoph Sommer and
• Thomas Scheibel

Beilstein J. Nanotechnol. 2022, 13, 902–921, doi:10.3762/bjnano.13.81

• materials using bioactive and bioadhesive molecules, such as full-length ECM proteins or functional peptide fragments thereof. These ligands interact with cell receptors for guiding cellular responses, such as cell proliferation or specific matrix degradation [31][32][33][34]. Compared to full-length

• worldwide. Natural spider silk has the potential to be used for tissue engineering and biomedical applications. For instance, NIH 3T3 cells showed increased adhesion and proliferation on fabricated, sterilized wovens made of native, unmodified spider dragline silk fibres from Nephila clavipes over five days

• a residue substitution of aspartate with glutamate thus rendering an identical charge distribution, was applied as a control. Cell culture studies using Balb 3T3 fibroblasts revealed that the RGD peptide enhanced primary cell attachment with increased spread morphology and proliferation on films

Antibacterial activity of a berberine nanoformulation

• Hue Thi Nguyen,
• Tuyet Nhung Pham,
• Anh-Tuan Le,
• Nguyen Thanh Thuy,
• Tran Quang Huy and
• Thuy Thi Thu Nguyen

Beilstein J. Nanotechnol. 2022, 13, 641–652, doi:10.3762/bjnano.13.56

• , respectively. No MBC was found for pure BBR up to the saturation concentration of 2.0 mg/mL. Figure 6 shows the proliferation of MRSA and E. coli O157:H7 in nutrient broth after treatment with pure BBR and BBR NPs at different concentrations between 0.1 and 5.0 mg/mL. The results were obtained by colony

• increased from 0.5 mg/mL to the saturation concentration of BBR (2.0 mg/mL). The comparison between pure BBR and BBR NPs indicates that the antibacterial activity of BBR NPs was slightly higher in the concentration range of 0.1–1.0 mg/mL. Notably, proliferation of MRSA was not observed after treatment with

• BBR NPs at a concentration of 2.0 mg/mL. BBR NPs at a concentration of 2.0 mg/mL and higher exhibited a strong antibacterial effect against MRSA. Pure BBR and BBR NPs show low inhibitory activity at concentrations between 0.1 and 2.0 mg/mL against E. coli O157:H7 (Figure 6b). The proliferation of E

Effects of substrate stiffness on the viscoelasticity and migration of prostate cancer cells examined by atomic force microscopy

• Xiaoqiong Tang,
• Yan Zhang,
• Jiangbing Mao,
• Yuhua Wang,
• Zhenghong Zhang,
• Zhengchao Wang and
• Hongqin Yang

Beilstein J. Nanotechnol. 2022, 13, 560–569, doi:10.3762/bjnano.13.47

• elastography, whose imaging is based on the differences in stiffness between the lesion and the adjacent healthy tissue. It was found that an external environment with a high stiffness value promotes PC-3 cell migration and proliferation by inducing yes-associated protein and tafazzin (YAP/TAZ) nuclear

• migration capacity, and cells on soft substrates (3 kPa) had the lowest migration capacity (Figure 2c). Cell proliferation assays also revealed that both cell lines had a significantly greater ability to proliferate on stiff substrates, and that the proliferation of prostate cancer cells significantly

• increased with increasing substrate stiffness. (Supporting Information File 1, Figure S2a,b). The migration and proliferation assays were performed by culturing the cells on the substrates for 48 h, digesting them, and then inoculating them onto 6- and 96-well plates. This suggests that after 48 h the

Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

• Zahra Nabizadeh,
• Mahmoud Nasrollahzadeh,
• Hamed Daemi,
• Mohamadreza Baghaban Eslaminejad,
• Ali Akbar Shabani,
• Mehdi Dadashpour,
• Majid Mirmohammadkhani and
• Davood Nasrabadi

Beilstein J. Nanotechnol. 2022, 13, 363–389, doi:10.3762/bjnano.13.31

• ]. The results showed that MSCs cultured on GMs in a dynamic culture displayed a faster proliferation rate and higher stemness properties compared with those in two dimensional (2D) and 3D static culture systems [31]. Moreover, culturing MSCs on GMs in a dynamic system with the chondrogenic induction

• and proliferation of dental pulp stem cells (DPSCs) as well as chondrogenesis compared with the delivery of a single growth factor [54]. In addition, researchers utilized NPs composed of PLGA and poly(N-isopropylacrylamide) (PNIPAM) for the controlled release of insulin-like growth factor I (IGF-I

• ]. Although the results showed no appreciable difference between NMS and MS scaffolds in terms of inducing redifferentiation, nanoscale patterning of the microfibers influenced cell proliferation. In another study, similar results were obtained regarding the effect of poly(ʟ,ᴅ-lactide) (PLDLA) microfibers or