Identification of physicochemical properties that modulate nanoparticle aggregation in blood

• Ludovica Soddu,
• Duong N. Trinh,
• Eimear Dunne,
• Dermot Kenny,
• Giorgia Bernardini,
• Ida Kokalari,
• Arianna Marucco,
• Marco P. Monopoli and
• Ivana Fenoglio

Beilstein J. Nanotechnol. 2020, 11, 550–567, doi:10.3762/bjnano.11.44

• SNPs was not determined here since the value is available in literature [31][32][33]. Physicochemical and proteomics characterisation of the nanoparticle/hard corona We then evaluated how the NP physicochemical properties would affect the biomolecular corona formation. For this purpose, we exposed the

Interactions at the cell membrane and pathways of internalization of nano-sized materials for nanomedicine

• Valentina Francia,
• Daphne Montizaan and
• Anna Salvati

Beilstein J. Nanotechnol. 2020, 11, 338–353, doi:10.3762/bjnano.11.25

• developed techniques that are available in cell biology to characterize pathways and that have not yet been used to study the uptake of nano-sized materials may provide novel insights into this difficult question. For instance, so-called OMICS approaches based on large-scale proteomics and full genome

Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles

• Katrin Partikel,
• Robin Korte,
• Dennis Mulac,
• Hans-Ulrich Humpf and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101

• serum; nanoparticles; poly(lactide-co-glycolic acid); protein corona; proteomics; Introduction Nanoparticle (NP)-based drug carrier systems offer outstanding opportunities in the treatment of many serious diseases. The unique physicochemical properties and the ability to bind a library of ligands make

• human serum indicate a higher level of cationic proteins in the corona (Figure 3 and Figure 4) [28]. Additionally, the hard-corona proteins were identified by a shotgun proteomics-based approach. The adsorbed proteins were tryptically digested and the resulting peptides were analyzed by LC–MS/MS and

• ultrapure water. Subsequently, the sample was transferred into a folded capillary cell and the zeta potential of the NP–protein complex was determined as described above. Identification of corona proteins by LC–MS/MS Corona proteins were identified using a shotgun proteomics-based approach that has become

A biofunctionalizable ink platform composed of catechol-modified chitosan and reduced graphene oxide/platinum nanocomposite

• Peter Sobolewski,
• Agata Goszczyńska,
• Małgorzata Aleksandrzak,
• Karolina Urbaś,
• Joanna Derkowska,
• Agnieszka Bartoszewska,
• Jacek Podolski,
• Ewa Mijowska and
• Mirosława El Fray

Beilstein J. Nanotechnol. 2017, 8, 1508–1514, doi:10.3762/bjnano.8.151

• proteomics, in particular) during the crucial process of diagnosis. Importantly, an ideal biosensor platform needs to not only be sensitive and specific, but also flexible and affordable [1]. The past ten years have seen the growth of several technologies that hold much promise for the field of biosensors

The eNanoMapper database for nanomaterial safety information

• Nina Jeliazkova,
• Charalampos Chomenidis,
• Philip Doganis,
• Bengt Fadeel,
• Roland Grafström,
• Barry Hardy,
• Janna Hastings,
• Markus Hegi,
• Vedrin Jeliazkov,
• Nikolay Kochev,
• Pekka Kohonen,
• Cristian R. Munteanu,
• Haralambos Sarimveis,
• Bart Smeets,
• Pantelis Sopasakis,
• Georgia Tsiliki,
• David Vorgrimmler and
• Egon Willighagen

Beilstein J. Nanotechnol. 2015, 6, 1609–1634, doi:10.3762/bjnano.6.165

• endpoints of interest, which can vary from physicochemical to proteomics assays, and to enable retrieving all this data with a single REST call. A bundle may include the nanomaterials and assay data from a single investigation as well as serve as a container for a set of NMs and for data (typically

In vitro and in vivo interactions of selected nanoparticles with rodent serum proteins and their consequences in biokinetics

• Wolfgang G. Kreyling,
• Stefanie Fertsch-Gapp,
• Martin Schäffler,
• Blair D. Johnston,
• Nadine Haberl,
• Christian Pfeiffer,
• Jörg Diendorf,
• Carsten Schleh,
• Stephanie Hirn,
• Manuela Semmler-Behnke,
• Matthias Epple and
• Wolfgang J. Parak

Beilstein J. Nanotechnol. 2014, 5, 1699–1711, doi:10.3762/bjnano.5.180

• spheres (AuNP), surface-modified with the same ionic ligand; as well as 5 nm AuNP with five different surface modifications on the binding to serum proteins by using proteomics analyses. We found that the binding of numerous serum proteins depended strongly on the physicochemical properties of the AuNP

• -separation by gel electrophoresis. Negative controls were performed on the test tube used for the last washing step as well as on control incubations without any AuNP. For protein identification a MALDI-TOF MS proteomics analyzer was used. Furthermore, proteins in gel bands were analyzed quantitatively by

• proteomics and cellular analyses of lung tissue exposed to AgNP and Ag ions as well as more realistic bacterial post-exposures. We used monodisperse, spherical, PVP-coated AgNP with a size of 70 nm which had been carefully characterized [26][27]. The AgNP were dispersed in degassed distilled water and the

Current state of laser synthesis of metal and alloy nanoparticles as ligand-free reference materials for nano-toxicological assays

• Christoph Rehbock,
• Jurij Jakobi,
• Lisa Gamrad,
• Selina van der Meer,
• Daniela Tiedemann,
• Ulrike Taylor,
• Wilfried Kues,
• Detlef Rath and
• Stephan Barcikowski

Beilstein J. Nanotechnol. 2014, 5, 1523–1541, doi:10.3762/bjnano.5.165

• proteomics seems to indicate that the time resolved changes in the composition of the corona are not that pronounced [110]. However, protein-nanoparticle interactions for totally ligand-free nanoparticles have not been extensively studied [68], though it is reported that protein deformation is more likely to

Porous polymer coatings as substrates for the formation of high-fidelity micropatterns by quill-like pens

• Michael Hirtz,
• Marcus Lyon,
• Wenqian Feng,
• Andrea E. Holmes,
• Harald Fuchs and
• Pavel A. Levkin

Beilstein J. Nanotechnol. 2013, 4, 377–384, doi:10.3762/bjnano.4.44

• spotted by this method onto flat porous polymer substrates. Keywords: microarrays; microscale printing; microspotting; polymeric porous support; polymethacrylate; quill-like pens; Introduction Microarrays are of immense importance in many fields of biological research (e.g., genomics, proteomics, and