Ultrathin water layers on mannosylated gold nanoparticles

• Maiara A. Iriarte Alonso,
• Jorge H. Melillo,
• Silvina Cerveny,
• Yujin Tong and
• Alexander M. Bittner

Beilstein J. Nanotechnol. 2025, 16, 2183–2198, doi:10.3762/bjnano.16.151

• and stability in solution, in terms of hydrodynamic diameter and NP surface charge, respectively. Spectroscopy techniques were used to analyze the chemical composition of the organic ligands locally. We used FTIR for the molecular fingerprint infrared region to find the characteristic peaks of the

• measurements [24]. They form stable and well-defined organic layers on oxides, for example, on oxidized silicon wafers or glass, where surface charge and hydrophilicity are controlled by selecting the appropriate end groups. We combined all the methods mentioned to carry out AFM at variable RH levels in our

• -AuNPs. In both cases, the NP surface charge in water at pH ~7 was approx. −20 mV. The isoelectric point of carboxylate PEG-capped particles is ~2.5; hence, around pH 7, they should exhibit a negative ZP. This is also compatible with a low carboxylate content, as citrate-capped AuNPs (with a higher

Rapid synthesis of highly monodisperse AgSbS₂ nanocrystals: unveiling multifaceted activities in cancer therapy, antibacterial strategies, and antioxidant defense

• Funda Ulusu,
• Adem Sarilmaz,
• Yakup Ulusu,
• Faruk Ozel and
• Mahmut Kus

Beilstein J. Nanotechnol. 2025, 16, 2105–2115, doi:10.3762/bjnano.16.145

• in this study, it is generally accepted that the surface charge of NCs plays a critical role in their colloidal stability, dispersion behavior, and biological interactions [22][32]. In cubic cuboargyrite phase, there are 14 polyhedra with antimony and silver at the center (Figure 1c). Herein, a

• direct ROS measurement and apoptosis marker assays (e.g., Annexin V/PI staining, caspase-3 activation) will be crucial for validating and further elucidating these mechanistic pathways. The surface charge and zeta potential of AgSbS2 NCs, which strongly influence their colloidal stability and cellular

Toward clinical translation of carbon nanomaterials in anticancer drug delivery: the need for standardisation

• Michał Bartkowski,
• Francesco Calzaferri and
• Silvia Giordani

Beilstein J. Nanotechnol. 2025, 16, 2092–2104, doi:10.3762/bjnano.16.144

• physicochemical attributes, such as surface charge, further modulate the overall biological profile of CNMs, underscoring the complex interplay between nanoscale properties and biological behaviour [39]. Moreover, post-synthesis surface modifications can substantially diversify the physicochemical properties of a

• atomic force microscopy. Surface charge plays a significant role in determining how CNMs interact with biological membranes and intracellular environments. It influences processes such as cellular uptake, cytotoxicity, and inflammatory signalling. For example, BSA-derived negatively charged CDs exhibited

• enhanced cellular uptake, whereas positively charged CDs elicited stronger cytokine responses [41]. Zeta potential measurements are routinely used to quantify surface charge, which can be tuned through appropriate surface functionalisation strategies. Stability is another essential aspect of NM evaluation

Laser ablation in liquids for shape-tailored synthesis of nanomaterials: status and challenges

• Natalie Tarasenka

Beilstein J. Nanotechnol. 2025, 16, 1963–1997, doi:10.3762/bjnano.16.137

PEGylated lipids in lipid nanoparticle delivery dynamics and therapeutic innovation

• Peiyang Gao

Beilstein J. Nanotechnol. 2025, 16, 1914–1930, doi:10.3762/bjnano.16.133

• properties of LNPs including particle size, surface charge, and encapsulation efficiency. Subsequent sections explore the roles of PEG lipids in modulating protein corona formation and cellular uptake. The latter parts highlight the potential of functionalized PEG lipids for targeted delivery and the

• -based LNPs formulated with varying DMPE-PEG2k content from 1 to 5 mol %, changes were observed in nanoparticle diameter, polydispersity index (PDI), surface charge, and payload encapsulation efficiency (EE%). Increasing the PEG lipid content led to a reduction in the hydrodynamic diameter of LNPs, with

• particle size decreasing from 173.9 nm at 1 mol % to 109.1 nm at 5 mol %. Meanwhile, the PDI increased modestly from 0.060 to 0.146 with the increase of PEG density from 1 to 5 mol %, indicating a slight broadening in size distribution and less LNP homogeneity at higher PEG levels. The surface charge on

Exploring the potential of polymers: advancements in oral nanocarrier technology

• Rousilândia de Araujo Silva,
• Igor Eduardo Silva Arruda,
• Luise Lopes Chaves,
• Mônica Felts de La Roca Soares and
• Jose Lamartine Soares Sobrinho

Beilstein J. Nanotechnol. 2025, 16, 1751–1793, doi:10.3762/bjnano.16.122

• therapeutic payload and intestinal permeability [17]. PNs represent a viable strategy for overcoming GIT barriers in oral drug delivery. The polymeric composition allows for diverse and sophisticated designs, with primary advantages including the ability to control size, shape, and surface charge. Furthermore

• overall release kinetics [24][32][33]. From a pharmacokinetic perspective, the size, surface charge, and morphology of PNs can modulate their transit through the GIT, and such properties can be tuned during nanoparticle preparation. The choice of manufacturing method considers not only these parameters

• or evaporation, which ultimately defines the nomenclature of each. The selection of suitable polymers is governed by their solubility profiles, interactions with solvents and drugs, and surface charge, which requires careful consideration to meet the therapeutic demands of oral delivery systems [41

Multifunctional anionic nanoemulsion with linseed oil and lecithin: a preliminary approach for dry eye disease

• Niédja Fittipaldi Vasconcelos,
• Almerinda Agrelli,
• Rayane Cristine Santos da Silva,
• Carina Lucena Mendes-Marques,
• Isabel Renata de Souza Arruda,
• Priscilla Stela Santana de Oliveira,
• Mércia Liane de Oliveira and
• Giovanna Machado

Beilstein J. Nanotechnol. 2025, 16, 1711–1733, doi:10.3762/bjnano.16.120

• droplet uniformity with a monodisperse profile [93]; (3) maximizing lecithin’s performance in forming LO droplets through monolayer micelles [81]; (4) avoiding excess surfactant molecules to maintain biocompatibility [94][95]; and (5) maintaining a negative surface charge density greater than −30 mV for

Prospects of nanotechnology and natural products for cancer and immunotherapy

• Jan Filipe Andrade Santos,
• Marcela Bernardes Brasileiro,
• Pamela Danielle Cavalcante Barreto,
• Ligiane Aranha Rocha and
• José Adão Carvalho Nascimento Júnior

Beilstein J. Nanotechnol. 2025, 16, 1644–1667, doi:10.3762/bjnano.16.116

Venom-loaded cationic-functionalized poly(lactic acid) nanoparticles for serum production against Tityus serrulatus scorpion

• Philippe de Castro Mesquita,
• Karla Samara Rocha Soares,
• Manoela Torres-Rêgo,
• Emanuell dos Santos-Silva,
• Mariana Farias Alves-Silva,
• Alianda Maira Cornélio,
• Matheus de Freitas Fernandes-Pedrosa and
• Arnóbio Antônio da Silva-Júnior

Beilstein J. Nanotechnol. 2025, 16, 1633–1643, doi:10.3762/bjnano.16.115

• nanoparticles was functionalized with PEI, a hydrophilic polymer that provides a positive surface charge. This modification enhances high macromolecule loading or improves the cell interaction and cellular uptake [22][33][34]. The cationic character induces biomolecules with negative charges, such as proteins

• functionalized cationic PLA nanoparticles adsorbed with negatively charged proteins was successfully developed by low-energy solvent diffusion method, producing effective and stable spherical cationic nanoparticles. The positive surface charge enabled a high protein incorporation into particles. The prolonged

Nanotechnology-based approaches for the removal of microplastics from wastewater: a comprehensive review

• Nayanathara O Sanjeev,
• Manjunath Singanodi Vallabha and
• Rebekah Rubidha Lisha Rabi

Beilstein J. Nanotechnol. 2025, 16, 1607–1632, doi:10.3762/bjnano.16.114

• results in a large specific surface area, which in turn offers the potential to control membrane pore size and enhance the removal efficiency of nanoparticles and MPs. Membranes developed using MOF-based nanomaterials offer scalable control over surface charge and pore size, enabling high separation

• six degrees of freedom due to their negative photogravitaxis. Their self-propelling capability, combined with tunable surface charge (zeta potential), enables rapid attraction and capture of nanoplastics onto their surfaces and between layered structures. The magnetic nature of the microrobots allows

Laser processing in liquids: insights into nanocolloid generation and thin film integration for energy, photonic, and sensing applications

• Akshana Parameswaran Sreekala,
• Pooja Raveendran Nair,
• Jithin Kundalam Kadavath,
• Bindu Krishnan,
• David Avellaneda Avellaneda,
• M. R. Anantharaman and
• Sadasivan Shaji

Beilstein J. Nanotechnol. 2025, 16, 1428–1498, doi:10.3762/bjnano.16.104

• careful control of several factors, such as particle surface charge, the electrical and fluid properties of the suspension, electrode material selection, and the current–voltage parameters. Additionally, it is a cost-effective, environmentally friendly method due to its low energy requirements and minimal

• megahertz range). The NPs were further analyzed regarding phase, composition, electrical surface charge, and charge modulation mechanisms. EPD of a Au colloidal solution prepared by laser ablation in different solvents has been reported [131]. Figure 14a–d presents the structural and morphological

The role of biochar in combating microplastic pollution: a bibliometric analysis in environmental contexts

• Tuan Minh Truong Dang,
• Thao Thu Thi Huynh,
• Guo-Ping Chang-Chien and
• Ha Manh Bui

Beilstein J. Nanotechnol. 2025, 16, 1401–1416, doi:10.3762/bjnano.16.102

• and surface area, compared to other materials [37]. Biochar produced from carbon-, oxygen- and nitrogen-rich straw at low pyrolysis temperatures retains a large number of functional groups, which enhance charge transfer potential and adsorption stability through increased surface charge density

Enhancing the therapeutical potential of metalloantibiotics using nano-based delivery systems

• Alejandro Llamedo,
• Marina Cano,
• Raquel G. Soengas and
• Francisco J. García-Alonso

Beilstein J. Nanotechnol. 2025, 16, 1350–1366, doi:10.3762/bjnano.16.98

• , significantly expanding their applications [58]. The structure of liposomes can vary, with unilamellar and multilamellar vesicles being the two main forms, offering flexibility in the types of drugs they can deliver. In addition, size, surface charge, and lipid composition of these nanoparticles can be tailored

Ferroptosis induction by engineered liposomes for enhanced tumor therapy

• Alireza Ghasempour,
• Mohammad Amin Tokallou,
• Mohammad Reza Naderi Allaf,
• Mohsen Moradi,
• Hamideh Dehghan,
• Mahsa Sedighi,
• Mohammad-Ali Shahbazi and
• Fahimeh Lavi Arab

Beilstein J. Nanotechnol. 2025, 16, 1325–1349, doi:10.3762/bjnano.16.97

• ][120]. 3.2 Characterization of liposomes Characterization of liposomes is important to ensure their quality and performance as drug delivery systems. Several techniques are commonly used to analyze the properties of liposomes, including size, lamellarity, surface charge, and EE [110][121]. Dynamic

• light scattering (DLS) is commonly used to determine liposome size and size distribution. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) can be used to image liposome morphology and determine lamellarity. Zeta potential measurements assess the surface charge of liposomes, which

• liposomes is preserved [110]. The surface charge of liposomes, often expressed as zeta potential, is an important parameter that influences their stability and interaction with biological systems. Electrophoretic light scattering is often used to measure the zeta potential of liposomes [110][125]. Cryo-TEM

Enhancing the photoelectrochemical performance of BiOI-derived BiVO₄ films by controlled-intensity current electrodeposition

• Huu Phuc Dang,
• Khanh Quang Nguyen,
• Nguyen Thi Mai Tho and
• Tran Le

Beilstein J. Nanotechnol. 2025, 16, 1289–1301, doi:10.3762/bjnano.16.94

• synergistic effects of enhanced light absorption, reduced charge recombination, and increased charge transport efficiency, which are facilitated by the optimized fabrication parameters. To investigate the influence of different chemical components on charge separation within the BiVO4 bulk and surface charge

Mechanical stability of individual bacterial cells under different osmotic pressure conditions: a nanoindentation study of Pseudomonas aeruginosa

• Lizeth García-Torres,
• Idania De Alba Montero,
• Eleazar Samuel Kolosovas-Machuca,
• Facundo Ruiz,
• Sumati Bhatia,
• Jose Luis Cuellar Camacho and
• Jaime Ruiz-García

Beilstein J. Nanotechnol. 2025, 16, 1171–1183, doi:10.3762/bjnano.16.86

• obtain the mechanical response from the membrane rigidity within a reversible regime. The advantage of mapping bacteria at low loading forces is that surface interactions between the tip and cell membrane can be analyzed, and information related to its adhesion or surface charge could be revealed with

• . On PA, a negative FA or repulsion of about 25 pN was obtained, while a positive adhesion FA of about 50 pN on the PLL coat was measured. These results are consistent with the information gained by ξ-potential measurements of PA when measured in Milli-Q water, where a negative surface charge for PA of

Synthesis of biowaste-derived carbon-dot-mediated silver nanoparticles and the evaluation of electrochemical properties for supercapacitor electrodes

• Navya Kumari Tenkayala,
• Chandan Kumar Maity,
• Md Moniruzzaman and
• Subramani Devaraju

Beilstein J. Nanotechnol. 2025, 16, 933–943, doi:10.3762/bjnano.16.71

• density, quick charge/discharge capacity, and an outstanding retention rate. Nanocarbons are the appropriate candidates for EDLCs due to their high surface charge-storage ability and high conductivity [8][9][10]. The development of electrode materials with an efficient EDLC and pseudocapacitor properties

• surface of the CDs were oxidized and converted to C=O groups [34]. The mechanism for the production of PG-CDs-AgNPs has been illustrated in Figure 3. The surface area of the supercapacitor electrode material is an important feature that can aid in surface charge storage. The specific surface area of the

• mechanism for PG-CDs-AgNPs, as evidenced by the quasi-rectangular CV profile [38]. The electrochemistry of any electrode material is purposefully controlled by the composition of the electrolyte. Since KCl is used as a neutral electrolyte, PG-CDs-AgNPs exhibit capacitive behavior through a surface-charge

Morphology and properties of pyrite nanoparticles obtained by pulsed laser ablation in liquid and thin films for photodetection

• Akshana Parameswaran Sreekala,
• Bindu Krishnan,
• Rene Fabian Cienfuegos Pelaes,
• David Avellaneda Avellaneda,
• Josué Amílcar Aguilar-Martínez and
• Sadasivan Shaji

Beilstein J. Nanotechnol. 2025, 16, 785–805, doi:10.3762/bjnano.16.60

• likelihood of collisions and agglomeration if the repulsive forces are insufficient compared to other solvents, or the material-specific properties of FeS2, such as its semiconductor nature, weaker surface charge, and potential surface reactivity [73]. These factors reduce the effectiveness of electrostatic

• or steric stabilization, leading to agglomeration and precipitation. This behavior contrasts with metallic Ag NPs, which remain stable in acetone due to stronger electrical double layers and surface charge. According to the literature, in the case of nanosecond (ns) laser pulses, the laser energy can

Nanomaterials in targeting amyloid-β oligomers: current advances and future directions for Alzheimer's disease diagnosis and therapy

• Shiwani Randhawa,
• Trilok Chand Saini,
• Manik Bathla,
• Rahul Bhardwaj,
• Rubina Dhiman and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2025, 16, 561–580, doi:10.3762/bjnano.16.44

Synthetic-polymer-assisted antisense oligonucleotide delivery: targeted approaches for precision disease treatment

• Ana Cubillo Alvarez,
• Dylan Maguire and
• Ruairí P. Brannigan

Beilstein J. Nanotechnol. 2025, 16, 435–463, doi:10.3762/bjnano.16.34

• ][106], architecture [107][108], degree of amine/phosphate complexation [109], and surface charge density of the polymers [110] strongly affect the transfection efficiency and toxicity of these cationic platforms. In this section of the review, we discuss the progress and challenges associated with the

• coworkers [128], study and synthesis of poly(propylene imine) (PPI) dendrimers for drug and gene delivery have grown exponentially [129][130]. PPI dendrimers are highly branched, tree-like macromolecules with well-defined structures and dense positively surface charge comprised of a backbone of tertiary

• and oligofectamine–ASO complexes; cytotoxicity appeared to be related to dendrimer generation and, therefore, their molecular weight. Later studies by Ziemba et al. confirmed that the genotoxicity of PPI dendrimers was surface charge-dependent through the analysis of fourth-generation dendrimers (PPI

Engineered PEG–PCL nanoparticles enable sensitive and selective detection of sodium dodecyl sulfate: a qualitative and quantitative analysis

• Soni Prajapati and
• Ranjana Singh

Beilstein J. Nanotechnol. 2025, 16, 385–396, doi:10.3762/bjnano.16.29

• . Characterization of PEG–PCL nanoparticles The synthesized PEG–PCL nanoparticles were characterized by their unique physicochemical properties, such as size and surface charge. The average hydrodynamic size, monodispersity, and surface charge of the nanoparticles were measured using the ZetaSizer (Nano ZS, Malvern

• , UK). Dried 10 mg/mL PEG–PCL NPs were dissolved in PBS and sonicated for 30 min. The sonicated sample was taken in a cuvette for measurement. The sample pH was in the range of 7.2–7.4. Separate cuvettes (DTS1072 and DTS0012) were used to measure the surface charge and average size of the nanoparticles

• physicochemical properties, such as size, shape, surface charge, and elemental composition using DLS, TEM, SEM, and XPS (Figure 2). The PEG–PCL NPs were characterized using a zetasizer (Nano ZS, Malvern, UK) to comprehensively assess their size, surface charge, monodispersity, and average hydrodynamic size

Development of a mucoadhesive drug delivery system and its interaction with gastric cells

• Ahmet Baki Sahin,
• Serdar Karakurt and
• Deniz Sezlev Bilecen

Beilstein J. Nanotechnol. 2025, 16, 371–384, doi:10.3762/bjnano.16.28

• percentages ranging from 45% to 73% [62]. They concluded that this difference was not only due to the charge of the nanoparticles but also due to the different sizes of the formulations. In a recent study, the effects of surface charge and chemistry of the nanoparticles on their penetration abilities were

Fabrication and evaluation of BerNPs regarding the growth and development of Streptococcus mutans

• Tuyen Huu Nguyen,
• Hong Thanh Pham,
• Kieu Kim Thanh Nguyen,
• Loan Hong Ngo,
• Anh Ngoc Tuan Mai,
• Thu Hoang Anh Lam,
• Ngan Thi Kim Phan,
• Dung Tien Pham,
• Duong Thuy Hoang,
• Thuc Dong Nguyen and
• Lien Thi Xuan Truong

Beilstein J. Nanotechnol. 2025, 16, 308–315, doi:10.3762/bjnano.16.23

• of nanoparticles, and preserves the sterility of the material [19][20]. In this study, sodium dodecyl sulfate, a negative ion surfactant, was adsorbed in the production process onto the surface of nanoparticles, resulting in negatively charged particles. Particles with the same surface charge repel

Radiosensitizing properties of dual-functionalized carbon nanostructures loaded with temozolomide

• Radmila Milenkovska,
• Nikola Geskovski,
• Dushko Shalabalija,
• Ljubica Mihailova,
• Petre Makreski,
• Dushko Lukarski,
• Igor Stojkovski,
• Maja Simonoska Crcarevska and
• Kristina Mladenovska

Beilstein J. Nanotechnol. 2025, 16, 229–251, doi:10.3762/bjnano.16.18

• formulations of the hybrid CN MWCNTs-G compared to the corresponding MWCNTs were characterized by a similar or slightly higher TMZ content, larger particle size, similar surface charge, and slightly faster TMZ release, which can be attributed to the planar structure of graphene that promotes TMZ binding to the

• surface on a larger scale. For the irradiated CNs, lower values for particle size, more positive values for surface charge, and accelerated TMZ release were observed, which could be explained by changes in the physicochemical characteristics of the prepared formulations upon irradiation. Significant

• higher cytotoxicity of the hybrid CN. Additional decrease in the viability of cells was observed when GBM cells pre-treated with the corresponding CNs were exposed to irradiation, which could be ascribed to changes in size, surface charge, and release kinetics of TMZ and to irradiation-induced changes in

Recent advances in photothermal nanomaterials for ophthalmic applications

• Jiayuan Zhuang,
• Linhui Jia,
• Chenghao Li,
• Rui Yang,
• Jiapeng Wang,
• Wen-an Wang,
• Heng Zhou and
• Xiangxia Luo

Beilstein J. Nanotechnol. 2025, 16, 195–215, doi:10.3762/bjnano.16.16

• order to limit the ablation to the ILM more precisely, it is necessary to avoid ICG obstruction and trapping by the vitreous collagen network (the surface charge of ICG NPs should be negative or neutral, and the size of the ICG NPs should be less than 550 nm), as well as to avoid ICG crossing the ILM to

• reach the retina (the size of the ICG NPs should be greater than 100 nm). Therefore, in subsequent studies, two types of ICG NPs were synthesized using lipids and poly (lactic-co-glycolic acid) (PLGA) [53]. Size, surface charge, and ICG concentration of the NPs were modulated by varying the synthesis