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Search for "trypsin" in Full Text gives 61 result(s) in Beilstein Journal of Nanotechnology.
Nanocarrier systems loaded with IR780, iron oxide nanoparticles and chlorambucil for cancer theragnostics
Beilstein J. Nanotechnol. 2024, 15, 180–189, doi:10.3762/bjnano.15.17
- Sigma-Aldrich. Dulbecco's Modified Eagle Medium (DMEM) (11965092), fetal bovine serum (FBS) (MT35010CV), antibiotic (15-240-062), and trypsin (25-200-056) were purchased from Gibco, Fisher Scientific. All other solvents and reagents were of chemical grade. Synthesis of iron oxide nanoparticles Iron
Development and characterization of potential larvicidal nanoemulsions against Aedes aegypti
Beilstein J. Nanotechnol. 2024, 15, 104–114, doi:10.3762/bjnano.15.10
- 10% bovine fetal serum and 100 μg/mL of penicillin G/streptomycin. Maintained at 37 °C with 5% CO2, the cells were grown until they reached a subconfluent density. To detach the cells, a 5 min trypsin treatment with TrypLE™ Express at 37 °C was performed, followed by inactivation using 0.3 mg/mL
- trypsin inhibitor. The cells were then centrifuged at 500g for 5 min, resuspended in DMEM, and placed overnight in 96-well microplates (200 μL/well, 1 × 106 cells/mL) at 37 °C with 5% CO2. After incubation, nanoemulsions, free terpenes, and surfactant solutions were administered at concentrations from 0.1
Berberine-loaded polylactic acid nanofiber scaffold as a drug delivery system: The relationship between chemical characteristics, drug-release behavior, and antibacterial efficiency
Beilstein J. Nanotechnol. 2024, 15, 71–82, doi:10.3762/bjnano.15.7
- scaffolds The cell culture medium was prepared by mixing 150 mL of Dulbecco’s modified eagle medium (DMEM) with 600 μL of 0.5 mg/mL trypsin in a Schott bottle. MA-104 cells were distributed into the wells of a 96-well plate in DMEM supplemented with 10% fetal bovine serum. The BBR NPs/PLA nanofiber scaffold
Nanostructured lipid carriers containing benznidazole: physicochemical, biopharmaceutical and cellular in vitro studies
Beilstein J. Nanotechnol. 2023, 14, 804–818, doi:10.3762/bjnano.14.66
- 50 µM) or the free drug were added. After 72 h of treatment, the cells were harvested with a trypsin/EDTA solution and processed for flow cytometry analysis using a BD Biosciences FACSCANTO II Flow Cytometer (Franklin Lakes, NJ, USA). Propidium iodide (Sigma, St. Louis, USA) was added to the cell
In search of cytotoxic selectivity on cancer cells with biogenically synthesized Ag/AgCl nanoparticles
Beilstein J. Nanotechnol. 2022, 13, 1505–1519, doi:10.3762/bjnano.13.124
- solution made of trypsin-EDTA was added and incubated at 37 °C for 3 min in a humidified incubator with 5% CO2 to produce a cell suspension. Trypsinized cells were reseeded in fresh medium at 105 cells/mL and incubated at 37 °C in a 5% CO2 humidified incubator. All reagents were purchased from Biowest
Hydroxyapatite–bioglass nanocomposites: Structural, mechanical, and biological aspects
Beilstein J. Nanotechnol. 2022, 13, 1490–1504, doi:10.3762/bjnano.13.123
- quantity of 0.25% trypsin/0.02% EDTA. The cells were transferred in a 75 cm2 flask and cultured in mesenchymal stem cell expansion medium under the same conditions, changing the cell culture medium every second day, until 70–90% cellular confluence. After detachment, the cells were split and cultured in
Gelatin nanoparticles with tunable mechanical properties: effect of crosslinking time and loading
Beilstein J. Nanotechnol. 2022, 13, 778–787, doi:10.3762/bjnano.13.68
- degradation of the particles using trypsin for 6 h. The results revealed a crosslinking-dependent loading, with increased FITC-dextran loading for a longer crosslinking. The initial incorporation of FITC-dextran had a significant influence on Young’s moduli. The correlation between Young’s moduli and the
- loading of FITC-dextran was determined by dissolving the gelatin network using enzymatic degradation. The fluorescence intensity of the resulting solution was measured [18]. Therefore, 5 mg of freeze-dried GNPs were dispersed in 5 mL PBS containing 2.5 mg trypsin at room temperature. After 6 h of
Effects of substrate stiffness on the viscoelasticity and migration of prostate cancer cells examined by atomic force microscopy
Beilstein J. Nanotechnol. 2022, 13, 560–569, doi:10.3762/bjnano.13.47
- solution, respectively. In order to allow for the acclimatisation of cells to different substrates, cells were inoculated and cultured on hydrogel substrates with stiffness values of 3, 19, and 35 kPa. Next, cells were digested with 0.25% trypsin after 48 h and continued in the hydrogel on the same
- substrate. This was done for three repeated passages. Cells were digested with trypsin and counted before other experiments were carried out [44]. Preparation of polyacrylamide (PAA) gel substrates The preparation of polyacrylamide hydrogels with different stiffness referred to the experimental method of
Stimuli-responsive polypeptide nanogels for trypsin inhibition
Beilstein J. Nanotechnol. 2022, 13, 538–548, doi:10.3762/bjnano.13.45
- inflammatory mediator trypsin. Two types of nanogels were prepared from linear synthetic polypeptides based on biocompatible and biodegradable poly[N5-(2-hydroxyethyl)-ʟ-glutamine-ran-N5-propargyl-ʟ-glutamine-ran-N5-(6-aminohexyl)-ʟ-glutamine]-ran-N5-[2-(4-hydroxyphenyl)ethyl)-ʟ-glutamine] (PHEG-Tyr) or
- different loading capacities for AAT with the maximum (20%) achieved with Nα-Lys-NG nanogel. In both cases, the nanogel depots demonstrated a burst release of AAT during the first 6 h, which could be favorable for quick inhibition of trypsin. A consequent pilot in vitro inhibition study revealed that both
- PHEG-Tyr and Nα-Lys-NG nanogels loaded with AAT successfully inhibited the enzymatic activity of trypsin. Furthermore, the inhibitory efficiency of the AAT-loaded nanogels was higher than that of only AAT. Interestingly, also non-loaded PHEG-Tyr and Nα-Lys-NG nanogels were shown to effectively inhibit
Ethosomal (−)-epigallocatechin-3-gallate as a novel approach to enhance antioxidant, anti-collagenase and anti-elastase effects
Beilstein J. Nanotechnol. 2022, 13, 491–502, doi:10.3762/bjnano.13.41
- (−)-Epigallocatechin-3-gallate, 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), SPC, trypsin-EDTA solution, dimethyl sulfoxide (DMSO) for cell culture, and penicillin/streptomycin solution were purchased from Sigma, USA. Carbopol 980 was purchased from the Abdi İbrahim Pharmaceutical, Industry and
Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles
Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6
- with 10% of FBS and 1% of penicillin solution. The cells were kept at 37 °C under 5% CO2 and were subcultured three times per week with 0.25% trypsin/EDTA. The cells were seeded onto 96-well microtiter plates (Greiner) at a concentration of 5 × 103 cells/well (HeLa and MCF7) and 10 × 103 cells/well
Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles
Beilstein J. Nanotechnol. 2021, 12, 1339–1364, doi:10.3762/bjnano.12.99
- Institute (RPMI-1640, 99.9%) medium, GPPS (2 mM ʟ-glutamine, 1 mM Na pyruvate, 100 U/mL penicillin, 100 µg/mL streptomycin, 98%), Triton X-100 (lab grade), Trizma base, trypsin/EDTA (lab grade), sulforhodamine B (dye content 75%), ethidium bromide (lab grade), sodium dodecyl sulfate (SDS, lab grade), 2',7
- ) with 10% CO2. The cells were harvested using trypsin/EDTA (0.5 mM) for 1 min at room temperature. In vitro cytotoxicity screening of drug-loaded MFe2O4 (M = Fe, Co, Ni, Zn) NPs The cytotoxicity screening of colloidal drug-loaded MFe2O4 (M = Fe, Co, Ni, Zn) NPs was performed by using the SRB assay in
pH-driven enhancement of anti-tubercular drug loading on iron oxide nanoparticles for drug delivery in macrophages
Beilstein J. Nanotechnol. 2021, 12, 1127–1139, doi:10.3762/bjnano.12.84
- /nanoparticle treatment the cells were trypsinized using 1X trypsin-EDTA (HiMedia, India). The cell counts of the trypsinized cells were then determined using the trypan blue assay. Briefly, a fixed volume of animal cell sample was taken and diluted using a 0.4% solution of trypan blue (HiMedia, India). The
Silver nanoparticles induce the cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells via telomere length extension
Beilstein J. Nanotechnol. 2021, 12, 786–797, doi:10.3762/bjnano.12.62
- by PBS and trypsinized with trypsin-EDTA. The 4 × 103 cells were seeded in each well of a 96-well plate with a total volume of 200 μL of cardiomyogenic differentiation medium of different concentrations (0, 1.25, 2.5, and 5 µg/mL) of Ag-NPs and incubated for 14 days at 37 °C in a humidified
PEG/PEI-functionalized single-walled carbon nanotubes as delivery carriers for doxorubicin: synthesis, characterization, and in vitro evaluation
Beilstein J. Nanotechnol. 2020, 11, 1728–1741, doi:10.3762/bjnano.11.155
- were obtained from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). Trypsin–EDTA solution (0.25% trypsin with 0.02% EDTA) was supplied by Beyotime Biotechnology (Beijing, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from Sigma-Aldrich (St
- wells, and the cells were trypsinized with trypsin–EDTA and washed with cold PBS for three times to remove the nanocarriers outside the cells. The cells were then collected and resuspended in 200 μL of PBS by centrifugation. The mean fluorescence intensity (MFI) was determined using FCM (BD Biosciences
Identification of physicochemical properties that modulate nanoparticle aggregation in blood
Beilstein J. Nanotechnol. 2020, 11, 550–567, doi:10.3762/bjnano.11.44
- -PAGE) instrument for 10 min before the protein bands were excised from the gel in order to allow the whole corona proteins to migrate into a single gel band. The proteins in the gel pieces were reduced with dithiothreitol, alkylated with iodoacetamide and digested with trypsin (Promega Corporation
- and protein N-terminal acetylation. A maximum of two missed trypsin cleavages were allowed in the database search. The false discovery rate for both peptides and proteins was set at 1%. After that, the ProteinGroup file from Maxquant was processed, filtered and analysed with Perseus software to
Luminescent gold nanoclusters for bioimaging applications
Beilstein J. Nanotechnol. 2020, 11, 533–546, doi:10.3762/bjnano.11.42
- electrostatic interaction was ruled out. Instead, the authors assumed that it might be a specific peptide motif of HSA that interacts with the bacterial cell wall. The trypsin digestion of Au-HSA NCs was studied and various fragments were identified using MALDI–MS. To confirm further whether the peptides can
- imaging [91]. Recently Duan et al. reported the synthesis of NIR-luminescent AuNCs capped with N-acetyl-ʟ-cysteine (NAC-CS) for long-time imaging [92]. The Au-NAC-CS NCs were insensitive to hydrogen peroxide and trypsin in contrast to Au NCs coated with BSA or other proteins, allowing for extended imaging
Brome mosaic virus-like particles as siRNA nanocarriers for biomedical purposes
Beilstein J. Nanotechnol. 2020, 11, 372–382, doi:10.3762/bjnano.11.28
- by flow cytometry (Figure 1B). The differences in the extent of cell internalization could be explained by the surface charge as revealed by zeta potential measurements. The flow cytometry analysis of cell internalization was also performed after trypsin treatment, which promotes detachment of the
Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery
Beilstein J. Nanotechnol. 2019, 10, 2579–2593, doi:10.3762/bjnano.10.249
- serum (FBS, Gibco, USA), 2 mM ʟ-glutamine (Gibco, USA), 100 units/mL penicillin and 100 mg/mL streptomycin (Gibco, USA). When the cell culture became subconfluent, it was passaged at a ratio of 1:20 using a 0.05% trypsin/EDTA solution. Cell viability assay Before performing the cell viability assay, the
pH-Controlled fluorescence switching in water-dispersed polymer brushes grafted to modified boron nitride nanotubes for cellular imaging
Beilstein J. Nanotechnol. 2019, 10, 2428–2439, doi:10.3762/bjnano.10.233
- % penicillin/streptomycin/ampicillin and incubated in a water-jacketed incubator in a 5% CO2, 95% air atmosphere at 37 °C. The cells were expanded in T-75 flasks to 80% confluence and then detached with trypsin and collected. A 1 mg/mL sample of P(AA-co-FA)-functionalized BNNTs dispersed in ultrapure water was
Design of a nanostructured mucoadhesive system containing curcumin for buccal application: from physicochemical to biological aspects
Beilstein J. Nanotechnol. 2019, 10, 2304–2328, doi:10.3762/bjnano.10.222
- ), sodium chloride was purchased from Nuclear (Diadema, SP, Brazil) and polyssorbate 80 (Tween 80®) came from Synth® (Diadema, SP, Brazil). Dulbecco’s Modified Eagle’s Medium high glucose (DMEM), fetal bovine serum (FBS), ʟ-glutamine, penicillin, streptomycin and trypsin
Gold-coated plant virus as computed tomography imaging contrast agent
Beilstein J. Nanotechnol. 2019, 10, 1983–1993, doi:10.3762/bjnano.10.195
- carbonate, sucrose, dimethyl sulfoxide (DMSO; 25%), 2-(N-morpholino)ethanesulfonic acid (MES) buffer, isopropanol, tetrachloroauric acid, trypsin, ethylenediaminetetraacetic acid (EDTA), HEPES sodium salt, Triton™ X-100, phosphate-buffered saline (PBS) tablets, and bovine serum albumin (BSA) were purchased
- of growth surface and were used between passages 2 and 3. Subculture occurred after 60–70% confluence after trypsinization (0.025% trypsin, 0.5 mM EDTA, 10 mM HEPES buffer pH 6.5). RAW264.7 cell labeling and confocal microscopy: Cells of a murine endothelial line (100 μL of 1 × 106 cells/mL, RAW264.7
Synthesis and potent cytotoxic activity of a novel diosgenin derivative and its phytosomes against lung cancer cells
Beilstein J. Nanotechnol. 2019, 10, 1933–1942, doi:10.3762/bjnano.10.189
- anticancer formulation for lung cancer. Experimental Materials 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), DNA-free RNaseA, and propidium iodide (PI) were purchased from Sigma-Aldrich (St Louis, USA). Di was obtained from Energy Chemical (Shanghai, China). RPMI 1640 medium and trypsin
Engineered superparamagnetic iron oxide nanoparticles (SPIONs) for dual-modality imaging of intracranial glioblastoma via EGFRvIII targeting
Beilstein J. Nanotechnol. 2019, 10, 1860–1872, doi:10.3762/bjnano.10.181
- Sigma (USA) and Cy7.5 NHS ester was purchased from Nanocs (USA). Fetal bovine serum (FBS), phosphate buffered saline (PBS), trypsin-EDTA (0.25%), high glucose Dulbecco’s modified Eagle’s medium (DMEM) and penicillin-streptomycin were purchased from Gibco (CA, USA). The MTT cell proliferation and
- qualitative analysis, the cells were washed with PBS (0.01 M, pH 7.4) three times, then DAPI-stained and observed under a fluorescence microscope (Leica, DMI4000B, Germany). For the quantitative examination of the cellular uptake of the nanoprobes, U87MG and U87MG-EGFRvIII cells were harvested after trypsin
Toxicity and safety study of silver and gold nanoparticles functionalized with cysteine and glutathione
Beilstein J. Nanotechnol. 2019, 10, 1802–1817, doi:10.3762/bjnano.10.175
- were regularly tested for absence of mycoplasma by means of a direct DNA dye test [85]. When the cells reached 80% confluence, the culture medium was removed with a pipette; the cells were washed once with sterile phosphate buffer saline (PBS), detached from the flask by adding trypsin/EDTA (0.25
- untreated cells were negative controls. After treatment, the plates were centrifuged at 1500g for 15 min. Supernatants containing dead cells were collected from 12-well plates in 1.5 mL Eppendorf tubes. Live cells were detached from the wells by adding 0.05 % GibcoTM trypsin/EDTA (Thermo Fisher Scientific
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