Orthogonal dual-modification of proteins for the engineering of multivalent protein scaffolds

Michaela Mühlberg, Michael G. Hoesl, Christian Kuehne, Jens Dernedde, Nediljko Budisa and Christian P. R. Hackenberger

Beilstein J. Org. Chem. 2015, 11, 784–791. doi:10.3762/bjoc.11.88

Supporting Information

Details on materials, protein design, construction of the expression plasmids, protein expression and purification, mass spectrometry data for the expressed proteins, general methods, synthetic protocols and analytical data (including 1H, 13C and 19F NMR spectra) for compounds 1 and 2, reaction conditions for the ligation strategies, SDS PAGE and Western Blot lanes are provided as Supporting Information.

Supporting Information File 1: Additional data.
Format: PDF Size: 1.2 MB Download
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