Polyketide stereocontrol: a study in chemical biology

Scholarly Works

• Kim, M.; Kim, H.; Kang, J. Y. Structural insights into modular polyketide synthases: From individual domains to supramolecular assemblies. Bulletin of the Korean Chemical Society 2025. doi:10.1002/bkcs.70081
• Chainani, Y.; Diaz, J.; Guilarte-Silva, M.; Blay, V.; Zhang, Q.; Sprague, W.; Tyo, K. E. J.; Broadbelt, L. J.; Mukhopadhyay, A.; Keasling, J. D.; Martin, H. G.; Backman, T. W. H. Merging the computational design of chimeric type I polyketide synthases with enzymatic pathways for chemical biosynthesis. Nature communications 2025, 16, 5787. doi:10.1038/s41467-025-61160-y
• Li, H.; Bai, Z.; Tabekoueng, G. B.; Xing, B.; Goldfuss, B.; Ma, M.; Yang, D.; Dickschat, J. S. A Hydrophobic Tunnel and a Gatekeeper at the Active Site Entrance in Two Type I Terpene Synthases. ACS Catalysis 2025, 15, 6209–6215. doi:10.1021/acscatal.5c01540
• Li, M.; Xu, J.; Zang, H.; Peng, W.-R.; Hong, L.-L.; Xu, C.; Li, W.; Jiao, W.-H.; Lin, H.-W.; Zhou, Y. Anti-Inflammatory α-Pyrone Polyethers Generated by Interrupting the Enolized 1,3-Diketone Formation in the Polyketide Synthases of Calcium-Ionophore Ionomycin. Organic letters 2025, 27, 1806–1811. doi:10.1021/acs.orglett.4c04711
• Nguyen, L. N. K. T.; Derra, S.; Hahn, F. The Relationship between Substrate Structure and Selectivity of Ketoreduction in Multimodular Polyketide Synthases: A Comparative Study of A-Type Ketoreductases from Late Modules Using Complex Precursor Analogues. ACS chemical biology 2025, 20, 186–196. doi:10.1021/acschembio.4c00669
• Chainani, Y.; Diaz, J.; Guilarte-Silva, M.; Blay, V.; Zhang, Q.; Sprague, W.; Tyo, K. E. J.; Broadbelt, L. J.; Mukhopadhyay, A.; Keasling, J. D.; Martin, H. G.; Backman, T. W. H. BioPKS Pipeline : An integrated platform for merging the computational design of chimeric type I polyketide synthases with enzymatic pathways for chemical biosynthesis. Cold Spring Harbor Laboratory 2024. doi:10.1101/2024.11.04.621673
• Xiang, C.; Yao, S.; Wang, R.; Zhang, L. Bioinformatic prediction of the stereoselectivity of modular polyketide synthase: an update of the sequence motifs in ketoreductase domain. Beilstein journal of organic chemistry 2024, 20, 1476–1485. doi:10.3762/bjoc.20.131
• Yin, Z.; Dickschat, J. S. Substrate specificity of a ketosynthase domain involved in bacillaene biosynthesis. Beilstein journal of organic chemistry 2024, 20, 734–740. doi:10.3762/bjoc.20.67
• Kimishima, A.; Tsuruoka, I.; Tsutsumi, H.; Honsho, M.; Honma, S.; Matsui, H.; Sugamata, M.; Wasuwanich, P.; Inahashi, Y.; Hanaki, H.; Asami, Y. A new tetronomycin analog, broad-spectrum and potent antibiotic against drug-resistant Gram-positive bacteria. Chemistry & biodiversity 2024, 21, e202301834. doi:10.1002/cbdv.202301834
• Leopold-Messer, S.; Chepkirui, C.; Mabesoone, M. F.; Meyer, J.; Paoli, L.; Sunagawa, S.; Uria, A. R.; Wakimoto, T.; Piel, J. Animal-associated marine Acidobacteria with a rich natural-product repertoire. Chem 2023, 9, 3696–3713. doi:10.1016/j.chempr.2023.11.003
• Hahn, F.; Derra, S.; Hoffmann, J. Synthesis of Biomimetic Thioesters for Studies of Ketoreductase Domains from the Biosynthesis of Cytotoxic Polyketides. Synlett 2023, 35, 1707–1712. doi:10.1055/a-2216-4521
• Yang, M.; Li, W.; Zhou, L.; Lin, X.; Zhang, W.; Shen, Y.; Deng, H.; Lin, H.-W.; Zhou, Y. Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual P450 monooxygenase-mediating aromatization and a putative metallo-beta-lactamase fold hydrolase. Synthetic and systems biotechnology 2023, 8, 349–356. doi:10.1016/j.synbio.2023.05.003
• Ogonkov, A.; Dieterich, C. L.; Meoded, R. A.; Piel, J.; Fraley, A. E.; Sasso, S. Characterization of an Unusual α-Oxoamine Synthase Off-Loading Domain from a Cyanobacterial Type I Fatty Acid Synthase. Chembiochem : a European journal of chemical biology 2023, 24, e202300209. doi:10.1002/cbic.202300209
• Niu, L.; Hu, W.-F.; Zhang, W.-J.; Lin, Z.-M.; Wang, J.-J.; Zhu, L.-L.; Hu, J.-Q.; Wang, C.-Y.; Li, R.-J.; Li, Y.-Z.; Wu, C. Genomics-Driven Discovery of Myxopyromides, a Class of Polyketidic Amides Associated with Predation ofMyxococcussp. SDU36. Cold Spring Harbor Laboratory 2023. doi:10.1101/2023.04.01.535179
• Yang, M.; Li, W.; Zhou, L.; Lin, X.; Zhang, W.; Shen, Y.; Deng, H.; Lin, H.-w.; Zhou, Y. Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual aromatization-mediating P450 monooxygenase and metallo-beta- lactamase fold thioesterase. Research Square Platform LLC 2023. doi:10.21203/rs.3.rs-2665340/v1
• Tan, B.; Zhang, Q.; Li, J.; Zhu, Y.; Zhang, L.; Zhang, W.; Zhang, H.; Chen, Y.; Zhang, C. Totopotensamide Congeners from a Halogenase-Inactivated Mutant. Journal of natural products 2023, 86, 76–84. doi:10.1021/acs.jnatprod.2c00772
• Wagner, L.; Stang, J.; Derra, S.; Hollmann, T.; Hahn, F. Towards understanding oxygen heterocycle-forming biocatalysts: a selectivity study of the pyran synthase PedPS7. Organic & biomolecular chemistry 2022, 20, 9645–9649. doi:10.1039/d2ob02064e
• Yin, Z.; Bär, D.; Gust, B.; Dickschat, J. S. The substrate scope of dehydratases in antibiotic biosynthesis and their application in kinetic resolutions. Organic & biomolecular chemistry 2022, 20, 9103–9107. doi:10.1039/d2ob01879a
• Dickschat, J. S. Enzymes in biosynthesis. Beilstein journal of organic chemistry 2022, 18, 1131–1132. doi:10.3762/bjoc.18.116
• Yin, Z.; Liebhart, E.; Stegmann, E.; Brötz-Oesterhelt, H.; Dickschat, J. S. An isotopic probe to follow the stereochemical course of dehydratase reactions in polyketide and fatty acid biosynthesis. Organic Chemistry Frontiers 2022, 9, 2714–2720. doi:10.1039/d2qo00272h

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