The enzymes of microbial nicotine metabolism

Scholarly Works

• Zuo, N.; Zuo, F.; Liu, Y.; Xiang, B. Genome Editing Using the Endogenous Type I-E CRISPR-Cas System in Lactobacillus paracasei ATCC334. Biotechnology and applied biochemistry 2025. doi:10.1002/bab.70056
• Zhang, Z.; Bandivadekar, P. R.; Gaunt, A. J.; Ahn, S.-H.; Barkman, T. J.; Stull, F. Ancestral evolution of oxidase activity in a class of (S)-nicotine and (S)-6-hydroxynicotine-degrading flavoenzymes. The Journal of biological chemistry 2025, 301, 110360. doi:10.1016/j.jbc.2025.110360
• Zhang, Z.; Freeland, K.; Stull, F. Role of glutamate 292 and lysine 331 in catalysis for the flavoenzyme (S)-6-hydroxynicotine oxidase from Shinella sp. HZN7. Archives of biochemistry and biophysics 2025, 771, 110492. doi:10.1016/j.abb.2025.110492
• Zhang, Z.; Freeland, K.; Stull, F. Role of glutamate 292 and lysine 331 in catalysis for the flavoenzyme (S)-6-hydroxynicotine oxidase from Shinella sp. HZN7. Cold Spring Harbor Laboratory 2025. doi:10.1101/2025.05.21.655333
• Kapaothong, Y.; Pimviriyakul, P. Structurally guided engineering of flavin-dependent nicotine dehydrogenase. Archives of biochemistry and biophysics 2025, 770, 110471. doi:10.1016/j.abb.2025.110471
• Hu, W.; Yuan, J.; Fei, J.; Imdad, K.; Yang, P.; Huang, S.; Mao, D.; Yang, J. Shaping the future of tobacco through microbial insights: a review of advances and applications. Frontiers in bioengineering and biotechnology 2025, 13, 1548323. doi:10.3389/fbioe.2025.1548323
• Zhang, Z.; Bandivadekar, P. R.; Gaunt, A. J.; Ahn, S.-H.; Barkman, T. J.; Stull, F. Ancestral evolution of oxidase activity in a class of (S)-nicotine and (S)-6-hydroxynicotine degrading flavoenzymes. Cold Spring Harbor Laboratory 2025. doi:10.1101/2025.04.24.649827
• El-Sabeh, A.; Mlesnita, A.-M.; Mihasan, M. Integrated transcriptomic and proteomic analysis of nicotine metabolism in Paenarthrobacter nicotinovorans ATCC 49919. International Biodeterioration & Biodegradation 2025, 199, 106017. doi:10.1016/j.ibiod.2025.106017
• Fitzpatrick, P. F. Conservation of mechanism in flavoprotein-catalyzed amine oxidation. Archives of biochemistry and biophysics 2024, 764, 110242. doi:10.1016/j.abb.2024.110242
• Yang, X.; Zhangyi, Z.; Yu, A.; Zhou, Q.; Xia, A.; Qiu, J.; Cai, M.; Chu, X.; Li, L.; Feng, Z.; Luo, Z.; Sun, G.; Zhang, J.; Geng, M.; Chen, S.; Xie, Z. GV-971 attenuates the progression of neuromyelitis optica in murine models and reverses alterations in gut microbiota and associated peripheral abnormalities. CNS neuroscience & therapeutics 2024, 30, e14847. doi:10.1111/cns.14847
• Chu, L. L.; My, L. Q.; Quang, H. N. Microbial alkaloids and their pharmaceutical and agricultural application. Fungal Secondary Metabolites; Elsevier, 2024; pp 73–90. doi:10.1016/b978-0-323-95241-5.00018-6
• Ye, C.; Liu, D.; Huang, K.; Li, D.; Ma, X.; Jin, Y.; Xiong, H. Isolation of starch and protein degrading strain Bacillus subtilis FYZ1-3 from tobacco waste and genomic analysis of its tolerance to nicotine and inhibition of fungal growth. Frontiers in microbiology 2023, 14, 1260149. doi:10.3389/fmicb.2023.1260149
• Ruzicka, J.; Julinova, M.; Rouchal, M.; Salac, J.; Vanharova, L.; Urban, J.; Pancochova, K. Degradation of antibacterial 1-octylpyrrolidin-2-one by bacterial pairs isolated from river water and soil. Environmental science and pollution research international 2022, 29, 45292–45302. doi:10.1007/s11356-022-19121-1
• Zhang, Z.; Mei, X.; He, Z.; Xie, X.; Yang, Y.; Mei, C.; Xue, D.; Hu, T.; Shu, M.; Zhong, W. Nicotine metabolism pathway in bacteria: mechanism, modification, and application. Applied microbiology and biotechnology 2022, 106, 889–904. doi:10.1007/s00253-022-11763-y
• Mihasan, M.; Boiangiu, R. S.; Guzun, D.; Babii, C.; Aslebagh, R.; Channaveerappa, D.; Dupree, E. J.; Darie, C. C. Time-Dependent Analysis of Paenarthrobacter nicotinovorans pAO1 Nicotine-Related Proteome. ACS omega 2021, 6, 14242–14251. doi:10.1021/acsomega.1c01020
• Tararina, M. A.; Dam, K. K.; Dhingra, M.; Janda, K. D.; Palfey, B. A.; Allen, K. N. Fast Kinetics Reveals Rate-Limiting Oxidation and the Role of the Aromatic Cage in the Mechanism of the Nicotine-Degrading Enzyme NicA2. Biochemistry 2021, 60, 259–273. doi:10.1021/acs.biochem.0c00855
• Dulchavsky, M.; Clark, C. T.; Bardwell, J. C.; Stull, F. A cytochrome c is the natural electron acceptor for nicotine oxidoreductase. Nature chemical biology 2021, 17, 344–350. doi:10.1038/s41589-020-00712-3
• Li, J.; Shen, M.; Chen, Z.; Pan, F.; Yang, Y.; Shu, M.; Chen, G.; Yang, J.; Zhang, F.; Linhardt, R. J.; Zhong, W. Expression and functional identification of two homologous nicotine dehydrogenases, NicA2 and Nox, from Pseudomonas sp. JY-Q. Protein expression and purification 2020, 178, 105767. doi:10.1016/j.pep.2020.105767
• Deay, D. O.; Colvert, K. K.; Gao, F.; Seibold, S.; Goyal, P.; Aillon, D. V.; Petillo, P. A.; Richter, M. L. An active site mutation in 6-hydroxy-l-Nicotine oxidase from Arthrobacter nicotinovorans changes the substrate specificity in favor of (S)-nicotine. Archives of biochemistry and biophysics 2020, 692, 108520. doi:10.1016/j.abb.2020.108520
• Brandsch, R.; Mihasan, M. A Soil Bacterial Catabolic Pathway on the Move: Transfer of Nicotine Catabolic Genes Between Arthrobacter Genus Megaplasmids and Invasion by Mobile Elements. Journal of biosciences 2020, 45, 1–12. doi:10.1007/s12038-020-00030-9

Explore citations to this article at