Cytochrome P450 monooxygenase-mediated tailoring of triterpenoids and steroids in plants

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• Meng, Z.; Dong, M.; Xiao, C.; Li, Y.; Zhang, Y.; Cui, J.; Wang, S.; Li, P.; Baleev, D.; Zhan, Y.; Yin, J. Screening CYP450 genes from Gleditsia japonica Miq. and identifying CYP710A157 and CYP71D752 functions in the catalysis of echinocystic acid and betulin. Physiologia plantarum 2025, 177, e70176. doi:10.1111/ppl.70176
• Li, R.; Guo, S.; Wang, D.; Yang, T.; Li, W.; Wang, J.; Huang, L.; Zhang, X.; Dai, Z. Elucidation of the plant progesterone biosynthetic pathway and its application in a yeast cell factory. Metabolic engineering 2025, 90, 197–208. doi:10.1016/j.ymben.2025.03.016
• Hakim, S. E.; Liu, S.; Herzog, R.; Arafa, A.; de Vries, J.; Dräger, G.; Franke, J. Expansion of the Stereochemical Space of Triterpenes by Mining Noncanonical Oxidosqualene Cyclases Across the Diversity of Green Plants. Journal of the American Chemical Society 2025, 147, 10320–10330. doi:10.1021/jacs.4c16956
• Zong, J.-F.; Guo, X.-X.; Zou, K.-K.; Cui, C.-J.; Hu, Z.-H.; Hou, R.-Y. Isolation and identification of triterpenoid saponins with antiproliferative and hemolytic activities from Camellia oleifera Abel seeds. Phytochemistry 2025, 235, 114476. doi:10.1016/j.phytochem.2025.114476
• Mendoza Lara, D. F.; Hernández-Caballero, M. E.; Terán, J. L.; Ramírez, J. S.; Carrasco-Carballo, A. Anticancer Activities of Natural and Synthetic Steroids: A Review. ACS omega 2025, 10, 7493–7509. doi:10.1021/acsomega.4c08577
• Zeng, W.; Li, H.; Liu, S.; Luo, Z.; Chen, J.; Zhou, J. Biosynthesis and bioactivities of triterpenoids from Centella asiatica: Challenges and opportunities. Biotechnology advances 2025, 80, 108541. doi:10.1016/j.biotechadv.2025.108541
• Shirke, H. A.; Darshetkar, A. M.; Naikawadi, V. B.; Kavi Kishor, P. B.; Nikam, T. D.; Barvkar, V. T. Genomics of sterols biosynthesis in plants: Current status and future prospects. Plant science : an international journal of experimental plant biology 2025, 353, 112426. doi:10.1016/j.plantsci.2025.112426
• Liu, S.-J.; Liu, Z.; Shao, B.-Y.; Li, T.; Zhu, X.; Wang, R.; Shi, L.; Xu, S.; Van de Peer, Y.; Xue, J.-Y. Deciphering the biosynthetic pathway of triterpene saponins in Prunella vulgaris. The Plant journal : for cell and molecular biology 2025, 121, e17220. doi:10.1111/tpj.17220
• Yang, Y.; Wang, H.; Wu, Z.; Wang, X.; Tong, Y.; Huang, W.; Liu, X.; Zhao, H.; Hu, Y.; Zhang, X. Identification and Engineering of UDP-rhamnosyltransferase fromTrillium tschonoskiifor Heterologous Biosynthesis of Polyphyllin II in Engineered Yeast. Cold Spring Harbor Laboratory 2025. doi:10.1101/2025.01.26.634900
• Yang, T.; Chen, J.; Shi, N.; Fan, B.; Yi, R.; Liang, S.; Ji, A.; Liu, Z.; Li, C.; Wang, Q.; Duan, L. Discovery and Functional Identification of 2,3-Oxidosqualene Cyclases and Cytochrome P450s in Triterpenoid Metabolic Pathways of Actinidia eriantha. Journal of agricultural and food chemistry 2024, 72, 27902–27911. doi:10.1021/acs.jafc.4c06937
• Luo, X.; Ye, Z.; Shi, X.; Hu, Z.; Shen, J.; You, L.; Huang, P.; Wang, G.; Zheng, L.; Li, C.; Zhang, Y. Comparative transcriptomic analysis provides insights into the regulation of root-specific saponin production in Panax japonicus. Scientific reports 2024, 14, 27572. doi:10.1038/s41598-024-78720-9
• Hakim, S. E.; Choudhary, N.; Malhotra, K.; Peng, J.; Arafa, A.; Bültemeier, A.; Friedhoff, R.; Bauer, M.; Witte, C.-P.; Herde, M.; Heretsch, P.; Pucker, B.; Franke, J. Phylogenomics and metabolic engineering reveal a conserved gene cluster in Solanaceae plants for withanolide biosynthesis. Cold Spring Harbor Laboratory 2024. doi:10.1101/2024.09.27.614867
• Tamura, K.; Chiba, H.; Bono, H. Triterpene RDF: Developing a database of plant enzymes and transcription factors involved in triterpene biosynthesis using the Resource Description Framework. Plant biotechnology (Tokyo, Japan) 2024, 41, 303–308. doi:10.5511/plantbiotechnology.24.0312c
• Winegar, P. H.; Hudson, G. A.; Dell, L. B.; Astolfi, M. C. T.; Reed, J.; Payet, R. D.; Ombredane, H. C. J.; Iavarone, A. T.; Chen, Y.; Gin, J. W.; Petzold, C. J.; Osbourn, A. E.; Keasling, J. D. Verazine biosynthesis from simple sugars in engineered Saccharomyces cerevisiae. Metabolic engineering 2024, 85, 145–158. doi:10.1016/j.ymben.2024.07.011
• Peng, Y.; Tang, Y.; Li, D.; Ye, J. The Growth-Promoting and Colonization of the Pine Endophytic Pseudomonas abietaniphila for Pine Wilt Disease Control. Microorganisms 2024, 12, 1089. doi:10.3390/microorganisms12061089
• Surendran, K.; Pradeep, S.; Pillai, P. P. Comparative transcriptome and metabolite profiling reveal diverse pattern of CYP-TS gene expression during corosolic acid biosynthesis in Lagerstroemia speciosa (L.) Pers. Plant cell reports 2024, 43, 122. doi:10.1007/s00299-024-03203-0
• Dias, S. L.; Chuang, L.; Liu, S.; Seligmann, B.; Brendel, F. L.; Chavez, B. G.; Hoffie, R. E.; Hoffie, I.; Kumlehn, J.; Bültemeier, A.; Wolf, J.; Herde, M.; Witte, C.-P.; D'Auria, J. C.; Franke, J. Biosynthesis of the allelopathic alkaloid gramine in barley by a cryptic oxidative rearrangement. Science (New York, N.Y.) 2024, 383, 1448–1454. doi:10.1126/science.adk6112
• Lertphadungkit, P.; Mekloy, P.; Bunsupa, S. A novel C-29 oxidase, CYP712D39, catalyzes the formation of bryonolic acid from isomultiflorenol in Trichosanthes cucumerina L. Research Square Platform LLC 2024. doi:10.21203/rs.3.rs-3896957/v1
• Tamura, K.; Chiba, H.; Bono, H. Triterpene RDF: Developing a database of plant enzymes and transcription factors involved in triterpene biosynthesis using the Resource Description Framework. Cold Spring Harbor Laboratory 2024. doi:10.1101/2024.01.08.574260
• Chen, X.; Hudson, G. A.; Mineo, C.; Amer, B.; Baidoo, E. E. K.; Crowe, S. A.; Liu, Y.; Keasling, J. D.; Scheller, H. V. Deciphering triterpenoid saponin biosynthesis by leveraging transcriptome response to methyl jasmonate elicitation in Saponaria vaccaria. Nature communications 2023, 14, 7101. doi:10.1038/s41467-023-42877-0

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