Chemoselective N-acylation of indoles using thioesters as acyl source

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Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
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  2. ‡ Equal contributors
Associate Editor: J. A. Murphy
Beilstein J. Org. Chem. 2022, 18, 89–94.
Received 18 Nov 2021, Accepted 30 Dec 2021, Published 10 Jan 2022
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The selective acylation of indoles often requires sensitive and reactive acyl chloride derivatives. Here, we report a mild, efficient, functional group tolerant, and highly chemoselective N-acylation of indoles using thioesters as a stable acyl source. A series of indoleamides have been obtained with moderate to good yields. In addition, heterocycles, such as carbazole, can also be used as nucleophiles in this reaction.


Molecules containing N-acylindoles have attracted wide attention in the synthetic polymers and pharmaceutical industry because of their unique structural, chemical, and biological properties [1]. For example, indomethacin is a nonselective inhibitor of COX1 and COX2, which is used for treating fever, pain and swelling [2]. Indole analog L-768242 exhibits nanomolar potencies (Ki) with superior selectivity for the hCB2 receptor over the human central cannabinoid (hCB1) receptor [3] (Figure 1).


Figure 1: Representative pharmaceuticals containing N-acylindole moieties.

Indole has multiple reactive sites, and chemoselective N- or C-functionalization of indoles is a challenging and important task [4,5]. Acylation of indoles frequently takes place at the C3 position because of the relatively strong electron cloud density. As N-acylated indoles are a widespread motif in many pharmaceuticals and natural products [6-8], selective N-acylation of indoles is very important. However, this process often requires unstable and reactive acyl chloride, which results in a poor functional group tolerance. Thus, developing a simple and efficient method for the synthesis of N-acylindoles becomes much attractive [9-12]. In 2009, Scheidt developed a dehydrogenative approach using indoles and alcohols catalyzed by tetrapropylammonium perruthenate [13] (Scheme 1, A1). In 2012, Sarpong successfully carried out chemoselective acylation of the N(sp2)–H bond by using a catalytic amount of DBU for the preparation of indoleamides [14] (Scheme 1, A2). Subsequently, Sundén reported an efficient chemoselective method for the synthesis of indoleamide by oxidative organocatalytic reaction of indole derivatives and conjugated aldehydes under NHC catalysis [15] (Scheme 1, A1).


Scheme 1: A) Strategies for the synthesis of N-acylindoles; B) thioester as dicarbonylation reagent; C) recent work of our group; D) this work.

In 2019, Jiang and co-workers reported a dicarbonylation of amine and aryl borates using α-ketothioester as a stable 1,2-dicarbonyl reagent [16] (Scheme 1, B). Recently, we applied S-methyl butanethioate in a Pd-catalyzed transthioetherification or transthioesterification of aryl halides for the synthesis of thioethers and thioesters [17] (Scheme 1, C). In addition, we also used this reagent to trap alkylcopper(I) intermediates and to form C−S bonds [18]. To the best of our knowledge, thioesters have not been developed as indole N-amidation reagent. Based on our continuous interest on thioesters, herein, we report an efficient and chemoselective protocol for the synthesis of indoleamide derivatives with thioesters as acyl source.

Results and Discussion

At the beginning of our studies, we selected 3-methyl-1H-indole (1a) and S-methyl butanethioate (2a) as the model substrates to establish this procedure. As shown in Table 1, different bases were tried to improve the amidation reaction, and Cs2CO3 was found the most suitable choice (Table 1, entry 1). NaOt-Bu can also be used in this reaction and 82% yield could be obtained. In addition, NaOH and K2CO3 were not suitable in this procedure (Table 1, entries 3 and 4). Also the reaction did not work in the absence of Cs2CO3 (Table 1, entry 5). Solvent screening indicated that xylene was the best choice, and DMF, THF, and MeOH were not suitable for this reaction (Table 1, entries 7–9). When Cs2CO3 was reduced from 3.0 equiv to 2.0 equiv, 85% of the desired product could be obtained (Table 1, entry 10). Subsequently, we conducted a temperature optimization and 73% of the product was observed at 100 °C (Table 1, entry 11).

Table 1: Optimization of the reaction conditionsa.

[Graphic 1]
Entry Variation from standard conditions Yield (%)b
1 none 97
2 NaOt-Bu instead of Cs2CO3 82
3 NaOH instead of Cs2CO3 trace
4 K2CO3 instead of Cs2CO3 trace
5 without Cs2CO3 NRc
6 toluene instead of xylene 89
7 DMF instead of xylene 0
8 THF instead of xylene 0
9 MeOH instead of xylene 0
10 2.0 equiv Cs2CO3 85
11 100 °C instead of 140 °C 73

aReaction conditions: 1a (0.2 mmol, 1.0 equiv), 2a (0.6 mmol, 3.0 equiv), Cs2CO3 (0.6 mmol, 3.0 equiv), xylene (2.0 mL), 140 °C, 12 h. bYield was determined by GC using n-dodecane as the internal standard. cNR = no reaction.

Under the optimized reaction conditions, the scope of the reaction by variation of indoles and thioesters was tested. As shown in Scheme 2, a variety of functional groups, such as -OMe, -F and -I, are tolerated providing the desired products with moderate to excellent yields (Scheme 2, 3ce). In addition, various methyl thioesters could also participate in this reaction smoothly (Scheme 2, 3gr). Interestingly, S-methyl benzothioate and S-methyl 4-methylbenzothioate could also take part in this reaction and converted into the corresponding products 3s and 3t in 93% and 96% yield, respectively. Notably, carbazole could also be acylated with thioesters and 84% yield of the desired product was obtained (Scheme 2, 3u).


Scheme 2: Reactions of thioesters and indoles. Reaction conditions: 1 (0.2 mmol, 1.0 equiv), 2 (0.6 mmol, 3.0 equiv), Cs2CO3 (0.6 mmol, 3.0 equiv), xylene (2.0 mL), 140 °C, 12 h. Isolated yields are shown.

With the established method for the N-acylation of indoles, a 2 mmol scale reaction was carried out. The reaction of 3-methyl-1H-indole (1a) and S-methyl butanethioate (2a) proceeded smoothly and 1-(3-methyl-1H-indol-1-yl)butan-1-one (3a) was obtained with 62% isolated yield (0.25 g, Scheme 3). The results indicate that this N-acylation reaction of indole has great potential in practical synthesis.


Scheme 3: Gram-scale experiment.

Some control experiments were conducted to explore the reaction mechanism of this transformation (Scheme 4). When S-methyl decanethioate (2i) was adopted without Cs2CO3, no decomposition product was observed (Scheme 4, reaction 1). When S-methyl decanethioate (2i) was treated under the standard reaction conditions, 56% of 2i was recovered (Scheme 4, reaction 2). Furthermore, without Cs2CO3, no desired product could be obtained (Scheme 4, reaction 3). These results indicate that Cs2CO3 plays an important role in the N-acylation process of indoles. The reaction of decanoic acid and 3-methyl-1H-indole (1a) was also conducted under the standard conditions, and no desired product was obtained, illustrating that 1-(3-methyl-1H-indol-1-yl)decan-1-one (3i) was not transformed from decanoic acid (4) (Scheme 4, reaction 4).


Scheme 4: Control experiments.

A plausible reaction mechanism has been proposed based on the results of the control experiments. As shown in Scheme 5, the reaction starts with a base-promoted deprotonation of indole forming intermediate A. In the next step nucleophilic substitution between intermediate A and 2a occurs to give the desired N-acylindole product and CsSCH3 as byproduct [19-21] (Scheme 5).


Scheme 5: Proposed mechanism.


In conclusion, a chemoselective N-acylation of synthetically valuable indoles has been developed by using thioesters as a stable acyl source, a variety of N-acylated indoles could be obtained efficiently. Beside indole, carbazole can also take part in this reaction.

Supporting Information

Supporting Information File 1: Experimental part and NMR spectra.
Format: PDF Size: 3.7 MB Download


The authors are thankful for the financial support from the National Natural Science Foundation of China (22101005), the National Key Research and Development Program of China (2021YFD1700100), the Natural Science Foundation of Anhui Province (1908085MC71), Key Research and Development Program of Anhui Province (202104a06020008), Key Experimental Foundation for Quality and Safety of Agricultural Products in Anhui Province.


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