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6 article(s) from Koksch, Beate

Position-dependent impact of hexafluoroleucine and trifluoroisoleucine on protease digestion

Susanne Huhmann,
Anne-Katrin Stegemann,
Kristin Folmert,
Damian Klemczak,
Johann Moschner,
Michelle Kube and
Beate Koksch

Full Research Paper
Published 22 Dec 2017

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1. Graphical Abstract
2. Figure 1: (a) Structures of isoleucine (1), leucine (2), and their fluorinated analogues 5,5,5-trifluoroisole...
  
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3. Figure 2: Percentage of substrate remaining after incubation for 120 min (left) and 24 h (right) with α-chymo...
  
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4. Figure 3: Cleavage positions observed in the digestion of library peptides with α-chymotrypsin.
  
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5. Figure 4: Percentage of substrate remaining after incubation for 120 min (left) and 24 h (right) with pepsin ...
  
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6. Figure 5: Cleavage positions for digestion of the different peptides with pepsin.
  
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7. Figure 6: Percentage of substrate remaining after incubation for 120 min (left) and 24 h (right) with elastas...
  
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8. Figure 7: Cleavage positions for digestion of the different peptides with elastase.
  
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9. Figure 8: Percentage of substrate remaining after incubation for 120 min (left) and 24 h (right) with protein...
  
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10. Figure 9: Cleavage positions found with proteinase K.
  
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11. Figure 10: Dimension of stabilization or destabilization upon TfIle or HfLeu incorporation compared to the non...
  
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Beilstein J. Org. Chem. 2017, 13, 2869–2882, doi:10.3762/bjoc.13.279

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Inhibition of peptide aggregation by means of enzymatic phosphorylation

Kristin Folmert,
Malgorzata Broncel,
Hans v. Berlepsch,
Christopher H. Ullrich,
Mary-Ann Siegert and
Beate Koksch

Full Research Paper
Published 18 Nov 2016

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1. Graphical Abstract
2. Scheme 1: Helical wheel projection of two parallel helical strands and primary structure of KFM6. The recogni...
  
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3. Figure 1: a) Time-dependent CD spectra and b) corresponding CD-minimum plot for random coil (black) and β-she...
  
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4. Figure 2: ThT-binding assay of of 15 µM KFM6 in 50 mM Tris/HCl buffer with 10 mM MgCl₂ at pH 7.5, 24 °C, and ...
  
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5. Figure 3: a) TEM micrograph of 15 µM KFM6 in 50 mM Tris/HCl buffer with 10 mM MgCl₂ at pH 7.5 and 24 °C. The ...
  
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6. Figure 4: a) Enzymatic phosphorylation of 15 µM KFM6 in time-dependent CD-minimum plots. Addition of both 200...
  
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Graphical Abstract

Beilstein J. Org. Chem. 2016, 12, 2462–2470, doi:10.3762/bjoc.12.240

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Impact of multivalent charge presentation on peptide–nanoparticle aggregation

Daniel Schöne,
Boris Schade,
Christoph Böttcher and
Beate Koksch

Full Research Paper
Published 15 May 2015

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1. Graphical Abstract
2. Figure 1: Helical wheel representation and sequences of the peptides used in this study.
  
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3. Figure 2: CD spectra of 30 µM peptide VW05, R1A3, R2A2, R2A3, R2A4 and R2A5 at (A) pH 9 and (B) pH 11 in 10 m...
  
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4. Figure 3: (A) TEM of 100 µM R2A2 in 10 mM Tris/HCl buffer, pH 9. Sample was negative stained with 2% PTA; def...
  
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5. Figure 4: (A) Dynamic light scattering of 0.05 µM Au/MUA nanoparticles at pH 9. (B) Cryo TEM image of 0.05 µM...
  
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6. Figure 5: CD spectra of 15 µM peptide in the presence 0.05 µM Au/MUA nanoparticles at pH 9 after (A) 0 hours ...
  
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7. Figure 6: Agarose gel of (A) VW05, (B) R1A3, and (C) R2A2 in the presence of 0.05 µM Au/MUA nanoparticles at ...
  
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8. Figure 7: Cryo TEM images of 100 µM R2A2 and 0.05 µM Au/MUA nanoparticles at pH 9 at a defocus of (A) −1.2 µm...
  
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Beilstein J. Org. Chem. 2015, 11, 792–803, doi:10.3762/bjoc.11.89

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Synthesis of enantiomerically pure (2S,3S)-5,5,5-trifluoroisoleucine and (2R,3S)-5,5,5-trifluoro-allo-isoleucine

Holger Erdbrink,
Elisabeth K. Nyakatura,
Susanne Huhmann,
Ulla I. M. Gerling,
Dieter Lentz,
Beate Koksch and
Constantin Czekelius

Full Research Paper
Published 02 Oct 2013

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1. Graphical Abstract
2. Scheme 1: Synthesis of optically active N-acyloxazolidinone 5 from 4,4,4-trifluorobutanoic acid. Conditions: ...
  
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3. Scheme 2: Synthesis of enantiomerically pure (2S,3S)-5-F₃Ile and (2R,3S)-5-F₃-allo-Ile. TMGA = 1,1,3,3-tetram...
  
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4. Figure 1: Retention times of Fmoc amino acids plotted against the van der Waals volume of their side chains. ...
  
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5. Figure 2: CD spectra of K-5-F₃Ile and K-Ile peptides (KX: Ac-YGGKAAAAKAXAAKAAAAK-NH₂). The K-4’-F₃Ile spectru...
  
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Beilstein J. Org. Chem. 2013, 9, 2009–2014, doi:10.3762/bjoc.9.236

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Investigation of the network of preferred interactions in an artificial coiled-coil association using the peptide array technique

Raheleh Rezaei Araghi,
Carsten C. Mahrenholz,
Rudolf Volkmer and
Beate Koksch

Full Research Paper
Published 25 Apr 2012

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1. Graphical Abstract
2. Figure 1: (a) Helical wheel representation of the tetrameric Acid-pp/B3β2γ helix bundle, (b) sequences of Aci...
  
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3. Figure 2: (a) Sequence for random mutation resulting in 1764 spots. The randomized positions are denoted by X_a...
  
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4. Figure 3: Glycine scanning of Acid-pp sequences. The substituted glycines are highlighted in red. Each sequen...
  
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5. Figure 4: Heat-map diagrams depicting the quantitatively measured SIs for Acid-pp sequences containing mutati...
  
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6. Figure 5: (a) The complete sequences of the selected α-mutants. (b) CD and (c) thermal denaturation spectra o...
  
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7. Figure 6: Size-exclusion chromatograms of equimolar mixtures of B3β2γ with (a) Acid-pp, (b) V^aV^dE^eE^g, (c) L^aL^d...
  
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Beilstein J. Org. Chem. 2012, 8, 640–649, doi:10.3762/bjoc.8.71

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Chemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis

Shijie Ye,
Allison Ann Berger,
Dominique Petzold,
Oliver Reimann,
Benjamin Matt and
Beate Koksch

Preliminary Communication
Published 20 Apr 2010

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1. Graphical Abstract
2. Figure 1: General strategy of using a chemically aminoacylated suppressor tRNA and an in vitro translation sy...
  
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3. Figure 2: Structures of non-canonical amino acids. a. (S)-ethylglycine, b. (S)-2-amino-4,4-difluorobutanoic a...
  
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4. Scheme 1: General scheme for the synthesis of an N-(4-pentenoyl) amino acid cyanomethyl ester.
  
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5. Scheme 2: General scheme of synthesis of mono-2′(3′)-O-[N-(4-pentenoyl)aminoacyl]-pdCpAs and 2′-3′-bis-O-[N-(...
  
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6. Figure 3: Denaturing PAGE of ligation products of truncated suppressor tRNA and fluorinated aminoacyl-pdCpAs ...
  
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Beilstein J. Org. Chem. 2010, 6, No. 40, doi:10.3762/bjoc.6.40

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