Beilstein J. Org. Chem.2009,5, No. 37, doi:10.3762/bjoc.5.37
fluorometabolism in S. cattleya.
Keywords: 5-fluoro-5-deoxyribose-1-phosphate; fluorometabolite biosynthesis; phosphonates; phostone; ribose-1-phosphate; Introduction
Fluoroacetate (1) and 4-fluorothreonine (2) are unusual secondary metabolites in that they contain a fluorine atom. They are elaborated by the
step in the biosynthesis involves the fluorinase, an enzyme that catalyses nucleophilic attack of fluoride ion on SAM (3) to generate 5′-FDA (4). The initial fluorinated product 5′-FDA (4) is then depurinated by a purine nucleotide phosphorylase (PNP) to give 5-fluoro-5-deoxyribose-1-phosphate (5
). This phosphate intermediate becomes the substrate for a ring opening isomerisation reaction converting 5-fluoro-5-deoxyribose-1-phosphate (5) to 5-fluoro-5-deoxyribulose-1-phosphate (6). Further processing to fluoroacetaldehyde (7) and then conversion to fluoroacetate (1) and 4-fluorothreonine (2
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Graphical Abstract
Scheme 1:
Biosynthetic pathway from fluoride ion to fluoroacetate 1 and 4-fluorothreonine 2 in S. cattleya [2].