Back to the future: Why we need enzymology to build a synthetic metabolism of the future

• Tobias J. Erb

Beilstein J. Org. Chem. 2019, 15, 551–557, doi:10.3762/bjoc.15.49

• enzyme (super)family. An example are reducing enoyl-CoA carboxylases that were for most of the time annotated as ordinary enoyl-CoA reductases, with which they are phylogenetically related [46][47]. Another example are RubisCO-like proteins [48] that are enolases [49], isomerases [50] and

• transcarboxylases [51], respectively, which are not capable of fixing CO2, but are still found very often misannotated as their CO2 fixing homologs RubisCO, with which they share a common evolutionary history [52]. A solution to overcome the problem of misannotation might come from novel computational tools that

Biocatalysis for the application of CO₂ as a chemical feedstock

• Apostolos Alissandratos and
• Christopher J. Easton

Beilstein J. Org. Chem. 2015, 11, 2370–2387, doi:10.3762/bjoc.11.259

• as a renewable chemical feedstock, greatly enabling a sustainable carbon bio-economy. Keywords: biocatalysis; carboxylase; CO2 transformation; formate dehydrogenase; RuBisCO; Introduction Depletion of fossil-fuel feedstocks and pollution resulting from their unsustainable processing and use

• many CO2-assimiliating enzymes evolved to be strictly anaerobic, and are limited to specific environments, while others tolerate O2 [45]. As a result, the environmental [CO2]/[O2] ratio is an important effector of enzymatic properties. RuBisCO and the Calvin cycle For many years, the Calvin cycle for

• (RuBisCO). The glycerate 2 is subsequently phosphorylated with ATP for the production of 1,3-bisphosphoglycerate (3), which is in turn reduced with NADPH to 3-phosphoglyceraldehyde (4). For every six equivalents of the aldehyde 4, one is diverted to carbohydrate biosynthesis, while the other five are used