Identification of the new prenyltransferase Ubi-297 from marine bacteria and elucidation of its substrate specificity

• Jamshid Amiri Moghaddam,
• Huijuan Guo,
• Karsten Willing,
• Thomas Wichard and
• Christine Beemelmanns

Beilstein J. Org. Chem. 2022, 18, 722–731, doi:10.3762/bjoc.18.72

• , and culture conditions: Maribacter sp. MS6 was initially isolated from the green macroalga Ulva mutabilis [29][30]. The strain was cultivated in 25 mL marine broth (Carl Roth) at 150 rpm and 28 °C for up to four days. For cloning experiments, E. coli DH5 alpha and the pJET 1.2 (Thermofisher) and

A new glance at the chemosphere of macroalgal–bacterial interactions: In situ profiling of metabolites in symbiosis by mass spectrometry

• Marine Vallet,
• Filip Kaftan,
• Veit Grabe,
• Fatemeh Ghaderiardakani,
• Simona Fenizia,
• Aleš Svatoš,
• Georg Pohnert and
• Thomas Wichard

Beilstein J. Org. Chem. 2021, 17, 1313–1322, doi:10.3762/bjoc.17.91

• morphogenesis. The green macroalga Ulva mutabilis (Chlorophyta) develops into a callus-like phenotype in the absence of its essential bacterial symbionts Roseovarius sp. MS2 and Maribacter sp. MS6. Spatially resolved studies are required to understand symbiont interactions at the microscale level. Therefore, we

• spectrometry (AP-SMALDI-HRMS), low-molecular-weight polar compounds were identified by comparative metabolomics in the chemosphere of Ulva. Choline (2-hydroxy-N,N,N-trimethylethan-1-aminium) was only determined in the alga grown under axenic conditions, whereas ectoine (1,4,5,6-tetrahydro-2-methyl-4

• distributions of metabolites and identifying specific symbiotic bacteria. Keywords: algae; AP-SMALDI; ectoine; holobiont; high-resolution mass spectrometry; mass spectrometry imaging; marine bacteria; Ulva; Introduction In intertidal zones with high temporal and spatial ecosystem variations, bacteria and

Breakdown of 3-(allylsulfonio)propanoates in bacteria from the Roseobacter group yields garlic oil constituents

• Anuj Kumar Chhalodia and
• Jeroen S. Dickschat

Beilstein J. Org. Chem. 2021, 17, 569–580, doi:10.3762/bjoc.17.51

• ]. For the macroalga Ulva mutabilis the presence of bacteria from the Roseobacter group is even mandatory for proper algal development, and 3-(dimethylsulfonio)propanoate (DMSP) is used as a chemotactic signal by the bacteria attracting them towards the algal host [12]. Many bacteria and fungi also

Acyl-group specificity of AHL synthases involved in quorum-sensing in Roseobacter group bacteria

• Lisa Ziesche,
• Jan Rinkel,
• Jeroen S. Dickschat and
• Stefan Schulz

Beilstein J. Org. Chem. 2018, 14, 1309–1316, doi:10.3762/bjoc.14.112

• . inhibens DSM17395 was isolated from seawater of larval cultures of the scallop Pecten maximus in Spain [25] and P. inhibens 2.10 stemmed from the surface of the green macroalga Ulva australis in Australia [31]. The results showed that P. inhibens 2.10 and P. inhibens DSM17395 produce the same four AHLs, 3

Conserved and species-specific oxylipin pathways in the wound-activated chemical defense of the noninvasive red alga Gracilaria chilensis and the invasive Gracilaria vermiculophylla

• Martin Rempt,
• Florian Weinberger,
• Katharina Grosser and
• Georg Pohnert

Beilstein J. Org. Chem. 2012, 8, 283–289, doi:10.3762/bjoc.8.30

• profiling, and bioassays. For large-scale purification G. vermiculophylla was collected at Norderhafen, Germany. The snail Echinolittorina peruviana was collected at Las Cruces, Chile, transferred to the lab and maintained in seawater at 10 °C on a diet of Ulva sp. Extraction and bioassays. Herbivory was