2-Methyl-2,4-pentanediol (MPD) boosts as detergent-substitute the performance of ß-barrel hybrid catalyst for phenylacetylene polymerization

• Julia Kinzel,
• Daniel F. Sauer,
• Marco Bocola,
• Marcus Arlt,
• Tayebeh Mirzaei Garakani,
• Andreas Thiel,
• Klaus Beckerle,
• Tino Polen,
• Jun Okuda and
• Ulrich Schwaneberg

Beilstein J. Org. Chem. 2017, 13, 1498–1506, doi:10.3762/bjoc.13.148

• demonstrate the importance of the right choice of the membrane protein stabilizer for biohybrid catalysis. Results and Discussion For solubilizing the transmembrane protein FhuA ΔCVFtev PE–PEG and MPD were applied as stabilizing agent and phenylacetylene polymerization was performed as model reaction (Figure

• precipitation leading to increased yields. The membrane protein FhuA is robust towards external influences such as increased temperatures and pH values. The catalyst and substrate scope in biohybrid catalysis can be fine-tuned choosing a suitable stabilizing agent as shown in this report. These results may

• inspire the tailoring of membrane proteins as catalysts in the field of biohybrid catalysis. Experimental General comments All used chemicals used were of analytical grade or higher quality, purchased from Applichem (Darmstadt, Germany) or Sigma-Aldrich Chemie (Taufkirchen, Germany). All operations were

Artificial Diels–Alderase based on the transmembrane protein FhuA

• Hassan Osseili,
• Daniel F. Sauer,
• Klaus Beckerle,
• Marcus Arlt,
• Tomoki Himiyama,
• Tino Polen,
• Akira Onoda,
• Ulrich Schwaneberg,
• Takashi Hayashi and
• Jun Okuda

Beilstein J. Org. Chem. 2016, 12, 1314–1321, doi:10.3762/bjoc.12.124

• : artificial Diels-Alderase; biohybrid catalysis; copper enyzme; membrane protein; Introduction So-called artificial metalloenzymes have attracted attention over the last decade [1][2][3][4][5][6][7][8][9]. Incorporation of an organometallic cofactor into proteins offers new possibilities to expand the