Beilstein J. Org. Chem.2017,13, 2408–2415, doi:10.3762/bjoc.13.238
Hui Hong Markiyan Samborskyy Katsiaryna Usachova Katharina Schnatz Peter F. Leadlay Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK 10.3762/bjoc.13.238 Abstract Clethramycin from Streptomyces malaysiensis DSM4137, and mediomycins (produced together with clethramycin
mediomycins are actually biosynthesised not by use of a different starter unit but by direct late-stage deamidination of (desulfo)clethramycin. A gene (slf) encoding a candidate sulfotransferase has been located in both gene clusters. Deletion of this gene in DSM4137 led to accumulation of desulfoclethramycin
only, instead of a mixture of desulfoclethramycin and clethramycin. The mediomycin gene cluster does not encode an amidinohydrolase, but when three candidate amidinohydrolase genes from elsewhere in the S. mediocidicus genome were individually expressed in Escherichia coli and assayed, only one of them
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Graphical Abstract
Scheme 1:
The structures of clethramycin, mediomycins and related linear polyenes.