Beilstein J. Org. Chem.2020,16, 956–965, doi:10.3762/bjoc.16.84
), and polyketide synthases (PKS). Selected Xenorhabdus and Photorhabdus mutant strains were generated applying a chemically inducible promoter in front of the suggested fabclavine (fcl) biosynthesis gene cluster (BGC), followed by the analysis of the occurring fabclavines. Subsequently, known and
unknown derivatives were identified and confirmed by MALDI–MS and MALDI–MS2 experiments in combination with an optimized sample preparation. This led to a total number of 22 novel fabclavine derivatives in eight strains, increasing the overall number of fabclavines to 32. Together with the identification
of fabclavines as major antibiotics in several entomopathogenic strains, our work lays the foundation for the rapid fabclavine identification and dereplication as the basis for future work of this widespread and bioactive SM class.
Keywords: antibiotic; fabclavine; NRPS-PKS hybrid; secondary
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Graphical Abstract
Figure 1:
General biosynthesis of fabclavine Ia (1) in X. budapestensis (A) and representation of a shortened...