Beilstein J. Org. Chem.2024,20, 607–620, doi:10.3762/bjoc.20.53
-position. Therefore, superscripts should be used to define the type of core fucose (Figure 4).
A structural element frequently occurring in mammalian glycoproteins, e.g., in immunoglobulins is the bisecting GlcNAc. This shall be added at the end of the abbreviation string by two lower case letters “bi”. In
PDF
Graphical Abstract
Figure 1:
Descriptions and depictions of a particular complex-type N-glycan structure found in human leukocyt...
Beilstein J. Org. Chem.2020,16, 3038–3051, doi:10.3762/bjoc.16.253
digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography–tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation. Initial glycopeptide identification based on MS/MS data was
quantification.
Keywords: bioinformatics; cysteine oxidation; glycoproteomics; immunoglobulins; mass spectrometry; Introduction
Protein glycosylation mainly occurs in the form of N- and O-glycosylation. N-Glycans are attached to Asn within an amino acid consensus sequence (Asn-Xxx-Ser/Thr, Xxx ≠ Pro) and O
this, a sample containing immunoglobulins G and A (IgG and IgA), simultaneously captured from human plasma, was chosen. This sample showed a considerable level of complexity due to the presence of multiple glycoproteins of interest and cocaptured (glyco)proteins from the plasma. The tools included
PDF
Graphical Abstract
Figure 1:
Integration of automated glycopeptide identification by Byonic and GlycopeptideGraphMS (aided by Op...