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Search for "microarrays" in Full Text gives 11 result(s) in Beilstein Journal of Organic Chemistry.

Elucidating the glycan-binding specificity and structure of Cucumis melo agglutinin, a new R-type lectin

  • Jon Lundstrøm,
  • Emilie Gillon,
  • Valérie Chazalet,
  • Nicole Kerekes,
  • Antonio Di Maio,
  • Ten Feizi,
  • Yan Liu,
  • Annabelle Varrot and
  • Daniel Bojar

Beilstein J. Org. Chem. 2024, 20, 306–320, doi:10.3762/bjoc.20.31

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  • -FY18-82). The sequence-defined glycan microarrays contain many saccharides provided by collaborators whom we thank, as well as members of the Glycosciences Laboratory for their contribution in the establishment of the NGL-based microarray system. This work benefited from access to EMBL HTX lab, which
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Published 19 Feb 2024

Chemical approaches to discover the full potential of peptide nucleic acids in biomedical applications

  • Nikita Brodyagin,
  • Martins Katkevics,
  • Venubabu Kotikam,
  • Christopher A. Ryan and
  • Eriks Rozners

Beilstein J. Org. Chem. 2021, 17, 1641–1688, doi:10.3762/bjoc.17.116

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Published 19 Jul 2021

The B & B approach: Ball-milling conjugation of dextran with phenylboronic acid (PBA)-functionalized BODIPY

  • Patrizia Andreozzi,
  • Lorenza Tamberi,
  • Elisamaria Tasca,
  • Gina Elena Giacomazzo,
  • Marta Martinez,
  • Mirko Severi,
  • Marco Marradi,
  • Stefano Cicchi,
  • Sergio Moya,
  • Giacomo Biagiotti and
  • Barbara Richichi

Beilstein J. Org. Chem. 2020, 16, 2272–2281, doi:10.3762/bjoc.16.188

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  • exploited in many applications including the selective sensing of saccharides, the separation and purification of glycoproteins, and the immobilization of antibodies in microarrays [17][18][19][20][21]. However, the synthesis of boronic esters is sometimes problematic, as it often requires a fine tuning of
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Published 11 Sep 2020

Tools for generating and analyzing glycan microarray data

  • Akul Y. Mehta,
  • Jamie Heimburg-Molinaro and
  • Richard D. Cummings

Beilstein J. Org. Chem. 2020, 16, 2260–2271, doi:10.3762/bjoc.16.187

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  • the mammalian body. They play a vital role in a number of physiologic and pathologic conditions. Glycan microarrays allow a plethora of information to be obtained on protein–glycan binding interactions. In this review, we describe the intricacies of the generation of glycan microarray data and the
  • biological systems have been discovered with respect to cancer, infectious diseases, and genetic disorders [7][8][9][10][11][12][13][14][15]. As a technology to study glycan recognition by GBPs, glycan microarrays offer an invaluable tool, and permit examination of all types of lectins, along with antibodies
  • . Glycans are recognized by many pathogens, including viruses and bacteria, and glycan microarrays are commonly now used to explore pathogen recognition of glycans [16][17][18][19][20][21]. Conversely, glycans from pathogens are also recognized by proteins in the human body and even produce an immune
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Published 10 Sep 2020

Stimuli-responsive oligonucleotides in prodrug-based approaches for gene silencing

  • Françoise Debart,
  • Christelle Dupouy and
  • Jean-Jacques Vasseur

Beilstein J. Org. Chem. 2018, 14, 436–469, doi:10.3762/bjoc.14.32

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  • thermolytic groups that are removed upon a ‘heat-driven’ process under neutral conditions. Modifications at the internucleotide linkage Over many years, various thermolytic groups for 5’-OH and phosphate protections have been designed and developed by Beaucage et al. to synthesize DNA ONs on microarrays due
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Published 19 Feb 2018

Glyco-gold nanoparticles: synthesis and applications

  • Federica Compostella,
  • Olimpia Pitirollo,
  • Alessandro Silvestri and
  • Laura Polito

Beilstein J. Org. Chem. 2017, 13, 1008–1021, doi:10.3762/bjoc.13.100

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  • signals [80]. The greatest advantage in using SERS is the possibility to provide unique spectral signatures describing glycan–protein interactions. Boons and co-workers prepared label-free microarrays of lactose-coated AuNPs (diameter of 60 nm) to study lactose galectin (i.e., galectine 1 and galectin 3
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Published 24 May 2017

DNA display of glycoconjugates to emulate oligomeric interactions of glycans

  • Alexandre Novoa and
  • Nicolas Winssinger

Beilstein J. Org. Chem. 2015, 11, 707–719, doi:10.3762/bjoc.11.81

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  • single strand stretches that remain flexible. Such assemblies can also be generated in spatially addressable format using DNA microarrays. At the core of this technology is the ability to conjugate biologically relevant glycans or glycomimetics to nucleic acids. Herein, we present an update of the
  • standard technology and customized arrays with 104–106 discrete sequences are readily available. Screening for glycan binding in a microarray format has also proven extremely valuable in glycobiology [46][47]. Based on earlier reports that small molecules [48][49] and protein microarrays [50] can be
  • for hybridization to DNA arrays and screened against lectins from pathogenic P. aeruginosa (PA2L and LecA) [51][52][53]. Our group used DNA microarrays to combinatorial pair diverse PNA-tagged glycan conjugates displayed at adjacent hybridization sites to produce assemblies emulating the diversity of
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Published 11 May 2015

Molecular recognition of surface-immobilized carbohydrates by a synthetic lectin

  • Melanie Rauschenberg,
  • Eva-Corrina Fritz,
  • Christian Schulz,
  • Tobias Kaufmann and
  • Bart Jan Ravoo

Beilstein J. Org. Chem. 2014, 10, 1354–1364, doi:10.3762/bjoc.10.138

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  • microcontact printed on epoxide-terminated self-assembled monolayers. Successive prints resulted in simple microarrays of two carbohydrates. The selectivity of the synthetic lectin was investigated by incubation on the immobilized carbohydrates. Selective binding of the synthetic lectin to immobilized NANA and
  • agglutinin. It was found that the printed carbohydrates retain their characteristic selectivity towards the synthetic and natural lectins and that the recognition of synthetic and natural lectins is strictly orthogonal. Keywords: carbohydrates; lectins; molecular recognition; microarrays; multivalent
  • sequencing, automated synthesis and high-throughput microarray screening are lacking in glycobiology [5]. However, during the last decade, these methods have also been adapted to carbohydrates [6][7][8]. Carbohydrate microarrays on chips proved to be a particularly useful tool in glycomics [9][10][11] since
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Published 16 Jun 2014

Acylsulfonamide safety-catch linker: promise and limitations for solid–phase oligosaccharide synthesis

  • Jian Yin,
  • Steffen Eller,
  • Mayeul Collot and
  • Peter H. Seeberger

Beilstein J. Org. Chem. 2012, 8, 2067–2071, doi:10.3762/bjoc.8.232

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  • product provides a terminal amine at the reducing end of the oligosaccharide for the fabrication of carbohydrate microarrays. An acylsulfonamide safety-catch linker 2 (Figure 1), was developed in combination with TentaGel resin to provide orthogonality to temporary ester protecting groups. This linker was
  • revealed during final deprotection serves in the formation of glycoconjugates and glycan microarrays [19]. Synthesis of 10 relied on key intermediates 4 and 6 (Scheme 1). Monobenzylated amine 4 was prepared by reductive amination [20]. An established three-step synthesis starting with hydrocinnamic acid
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Published 26 Nov 2012

Synthetic glycopeptides and glycoproteins with applications in biological research

  • Ulrika Westerlind

Beilstein J. Org. Chem. 2012, 8, 804–818, doi:10.3762/bjoc.8.90

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  • microarray format are desirable for such binding studies. Evaluation of antibody binding epitopes in vaccine and biomarker discovery is one recent example of glycopeptide microarrays [93][94]. Multivalency and orientation for glycan presentation have been shown to be important for the inhibition of microbe
  • development of methods in the synthesis of homogenous glycoproteins may result in new therapeutic applications. Better understanding of protein glycosylation will help to identify new protein targets for immunotherapy. The synthesis and application of glycopeptide microarrays is an upcoming topic, which will
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Published 30 May 2012

Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS

  • Martin J. Weissenborn,
  • Johannes W. Wehner,
  • Christopher J. Gray,
  • Robert Šardzík,
  • Claire E. Eyers,
  • Thisbe K. Lindhorst and
  • Sabine L. Flitsch

Beilstein J. Org. Chem. 2012, 8, 753–762, doi:10.3762/bjoc.8.86

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  • ; Introduction Microarrays have become valuable tools in the high-throughput analysis of biological interactions and have promising applications for the development of diagnostic devices in clinical environments [1]. The initial success with DNA microarrays has prompted investigations into other biomolecular
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Published 21 May 2012
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