Identification and isolation of insecticidal oxazoles from Pseudomonas spp.

• Florian Grundmann,
• Veronika Dill,
• Andrea Dowling,
• Aunchalee Thanwisai,
• Edna Bode,
• Narisara Chantratita,
• Richard ffrench-Constant and
• Helge B. Bode

Beilstein J. Org. Chem. 2012, 8, 749–752, doi:10.3762/bjoc.8.85

• intermediates were also investigated revealing interesting biological activities for several compounds despite their overall simple structures. Keywords: insecticidal activity; labradorin; oxazole; Pseudomonas; secondary metabolite; Findings During our search for novel natural products from entomopathogenic

Mutational analysis of a phenazine biosynthetic gene cluster in Streptomyces anulatus 9663

• Orwah Saleh,
• Katrin Flinspach,
• Lucia Westrich,
• Andreas Kulik,
• Bertolt Gust,
• Hans-Peter Fiedler and
• Lutz Heide

Beilstein J. Org. Chem. 2012, 8, 501–513, doi:10.3762/bjoc.8.57

• endophenazine B as a minor product (Figure 1) [11]. In the present study, we carried out inactivation experiments of genes on cosmid ppzOS04, followed by heterologous expression of the modified clusters and chemical analysis of secondary metabolite formation. This allowed us to investigate the function of

• quantitative differences in production reliably. We decided to use cultivation in 24 square deep-well plates (EnzyScreen BV, The Netherlands). Previous studies have shown that this greatly reduces the variability of secondary metabolite production in comparison to cultivation in Erlenmeyer flasks [14]. In

• shown to increase the production of certain antibiotics [14]. Of each mutant obtained in this study, usually three independent clones were isolated, and secondary metabolite production was determined in three parallel cultivations for each clone. The variability of production between different clones