Hemolysin coregulated protein 1 as a molecular gluing unit for the assembly of nanoparticle hybrid structures

• Tuan Anh Pham,
• Andreas Schreiber,
• Elena V. Sturm (née Rosseeva),
• Stefan Schiller and
• Helmut Cölfen

Beilstein J. Nanotechnol. 2016, 7, 351–363, doi:10.3762/bjnano.7.32

• % glycerol) of proteins (supplied by the Schreiber group) was removed with PD Spin Trap G-25 from GE Healthcare. The proteins were dissolved in water with 100 mM NaCl. Au NP Hcp1_cys3 network The Au NPs were used directly after synthesis. The NP concentration was determined by measuring the absorbance of the

Peptide-equipped tobacco mosaic virus templates for selective and controllable biomineral deposition

• Klara Altintoprak,
• Axel Seidenstücker,
• Alexander Welle,
• Sabine Eiben,
• Petia Atanasova,
• Nina Stitz,
• Alfred Plettl,
• Joachim Bill,
• Hartmut Gliemann,
• Holger Jeske,
• Dirk Rothenstein,
• Fania Geiger and
• Christina Wege

Beilstein J. Nanotechnol. 2015, 6, 1399–1412, doi:10.3762/bjnano.6.145

• example, as a structural modifier or filler in rubber [1], food [2][3] or healthcare products [4], bioceramics for medical purposes [5], mesoporous nanoparticulate or tubular drug delivery systems as reviewed in [6], sensor surfaces [7], or biocatalytic formulations as reviewed in [8]. An important focus

Functionalization of α-synuclein fibrils

• Simona Povilonienė,
• Vida Časaitė,
• Virginijus Bukauskas,
• Arūnas Šetkus,
• Juozas Staniulis and
• Rolandas Meškys

Beilstein J. Nanotechnol. 2015, 6, 124–133, doi:10.3762/bjnano.6.12

• ), containing 0.1 mM EDTA, 0.2 mM PMSF, and 500 mM NaCl. The cell lysate was heated at 100 °C for 10 min and subsequently centrifuged at 13200g for 20 min. The supernatant was dialyzed against 50 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 1 mM EDTA, pH 8.0, and loaded into a HiTrap ANX column (GE Healthcare

• ) equilibrated in the same buffer. Proteins were eluted using a linear gradient of 0–1 M NaCl. Fractions containing α-SynC141 were pooled and additionally applied to a HiTrap Q XL column (GE Healthcare) and were identified by 15% SDS-PAGE. The α-Syn protein, expressed and purified in the same manner as α-SynC141

Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells

• Heike Sinnecker,
• Katrin Ramaker and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239

• . The biotinylated lectin RCA I was used in a concentration of 20 µg/mL in 0.2% gelatin/PBS to label the cells over night at 4 °C. Afterwards the cells were incubated with Cy5-conjugated streptavidin (GE Healthcare, Brand Amersham, Pittsburgh, USA), 5 µg/mL in 0.2% gelatin/PBS, for 90 min at rt. Finally

Effect of silver nanoparticles on human mesenchymal stem cell differentiation

• Christina Sengstock,
• Jörg Diendorf,
• Matthias Epple,
• Thomas A. Schildhauer and
• Manfred Köller

Beilstein J. Nanotechnol. 2014, 5, 2058–2069, doi:10.3762/bjnano.5.214

• (Ultraspec 3100 pro, Amersham Bioscience, GE Healthcare, Freiburg Germany) at a wavelength of 520 nm to measure the optical density of the extracted oil droplets. For Bodipy493/503 fluorescence quantification microphotographs were taken (Olympus CellP, Olympus, Hamburg, Germany) and digitally processed using

Silica nanoparticles are less toxic to human lung cells when deposited at the air–liquid interface compared to conventional submerged exposure

• Alicja Panas,
• Andreas Comouth,
• Harald Saathoff,
• Thomas Leisner,
• Marco Al-Rawi,
• Michael Simon,
• Gunnar Seemann,
• Olaf Dössel,
• Sonja Mülhopt,
• Hanns-Rudolf Paur,
• Susanne Fritsch-Decker,
• Carsten Weiss and
• Silvia Diabaté

Beilstein J. Nanotechnol. 2014, 5, 1590–1602, doi:10.3762/bjnano.5.171

• ) detection system from GE Healthcare (Freiburg, Germany) were used. Transwell® inserts containing polyester membranes of 24 mm diameter and with pores of 0.4 µm (Cat.No. 3450) were from Corning Life Sciences (Amsterdam, The Netherlands). 75-mesh formvar-coated copper grids for transmission electron

The cell-type specific uptake of polymer-coated or micelle-embedded QDs and SPIOs does not provoke an acute pro-inflammatory response in the liver

• Markus Heine,
• Alexander Bartelt,
• Oliver T. Bruns,
• Denise Bargheer,
• Artur Giemsa,
• Barbara Freund,
• Ludger Scheja,
• Christian Waurisch,
• Alexander Eychmüller,
• Rudolph Reimer,
• Horst Weller,
• Peter Nielsen and
• Joerg Heeren

Beilstein J. Nanotechnol. 2014, 5, 1432–1440, doi:10.3762/bjnano.5.155

• isolated by gel chromatography using a PD-10 column (GE Healthcare). We injected 200 µL per mouse of QDs–lipid micelles (lipid concentration of QDs-lipid micelles stock solution was 10 mg/mL) or 200 µL of DiD–LDL per mouse (protein concentration of DiD–LDL was 2 mg/mL, total lipid concentration of DiD–LDL

Antimicrobial properties of CuO nanorods and multi-armed nanoparticles against B. anthracis vegetative cells and endospores

• Pratibha Pandey,
• Merwyn S. Packiyaraj,
• Himangini Nigam,
• Gauri S. Agarwal,
• Beer Singh and
• Manoj K. Patra

Beilstein J. Nanotechnol. 2014, 5, 789–800, doi:10.3762/bjnano.5.91

• nanoparticle based deactivation of microbes for medical and healthcare applications with nano-silver taking lead in academic research as well as in commercialization [9][10][11][12]. Comparatively few reports are available on the evaluation of nanoparticles against biological warfare (BW) agents including

Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats

• Nadine Haberl,
• Stephanie Hirn,
• Alexander Wenk,
• Jörg Diendorf,
• Matthias Epple,
• Blair D. Johnston,
• Fritz Krombach,
• Wolfgang G. Kreyling and
• Carsten Schleh

Beilstein J. Nanotechnol. 2013, 4, 933–940, doi:10.3762/bjnano.4.105

• after the instillation of 250 µg PVP-AgNP (Figure 8). Discussion According to the Woodrow–Wilson-Center database of nanotechnology-based products [28], silver is one of the most frequently used nanomaterials for consumer products. Due to the use of AgNP as aerosols in healthcare and hygiene spray

Controlled synthesis and tunable properties of ultrathin silica nanotubes through spontaneous polycondensation on polyamine fibrils

• Jian-Jun Yuan,
• Pei-Xin Zhu,
• Daisuke Noda and
• Ren-Hua Jin

Beilstein J. Nanotechnol. 2013, 4, 793–804, doi:10.3762/bjnano.4.90

• important for various applications, such as hydrogen storage [1], healthcare [2] and environmental technology [3]. It is well known that tubular silica structures can be fabricated by using inorganic [4], organic [5] or biological templates [6]. Among them, the utilization of self-assembled organic

Paper modified with ZnO nanorods – antimicrobial studies

• Mayuree Jaisai,
• Sunandan Baruah and
• Joydeep Dutta

Beilstein J. Nanotechnol. 2012, 3, 684–691, doi:10.3762/bjnano.3.78

• . Figure 8 shows digital images of the untreated paper and a sample of the antimicrobial paper grown at a concentration of 20 mM for 20 h with hand-written and printed text. The antimicrobial paper can therefore be used for various healthcare applications such as face masks, writing and printing paper, and

Detection of interaction between biomineralising proteins and calcium carbonate microcrystals

• Hanna Rademaker and
• Malte Launspach

Beilstein J. Nanotechnol. 2011, 2, 222–227, doi:10.3762/bjnano.2.26

• column (Sephadex TMG-25 M, GE Healthcare) to remove the SDS and DTT from the solutions. Beforehand the gel columns had been equilibrated with 25 mL 10 mM Tris-HCl (pH 8.0). After determining the fractions of highest protein concentration by a Bradford Assay (Bio-Rad), fractions 5 to 9 of two gel